Physical and chemical profiles of nanoparticles for lymphatic targeting

Ke, Xiyu; Howard, Gregory P.; Tang, Haoyu; Cheng, Bei; Saung, May Tun; Santos, José Lúıs; Mao, Hai Quan

doi:10.1016/j.addr.2019.09.005

Cited by 92 publications

(49 citation statements)

References 157 publications

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“…Biologically active nanoparticles would be applicable as immunotherapeutic tools such as vaccines and adjuvants against not only a wide range of infectious diseases (Kaparakis‐Liaskos and Ferrero, 2015), but also many other diseases including cancers and allergic diseases (Kishimoto and Maldonado, 2018; Ke et al , 2019). Among various possible nanoparticle‐based vaccines, bacterial MVs could be developed as novel drug delivery systems for use in medical and healthcare settings.…”

Section: Discussionmentioning

confidence: 99%

Glycine significantly enhances bacterial membrane vesicle production: a powerful approach for isolation of LPS‐reduced membrane vesicles of probiotic Escherichia coli

Hirayama

Nakao

2020

Microbial Biotechnology

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Bacterial membrane vesicles (MVs) have attracted strong interest in recent years as novel nanoparticle delivery platforms. Glycine is known to induce morphological changes in the outer layer of bacteria. We report here that glycine dramatically facilitates MV production in a flagella-deficient mutant of the nonpathogenic probiotic Escherichia coli strain Nissle 1917. Supplementation of culture medium with 1.0% glycine induced cell deformation at the early exponential phase, eventually followed by quasi-lysis during the late exponential to stationary phase. Glycine supplementation also significantly increased the number of MVs with enlarged particle size and altered the protein profile with an increase in the inner membrane and cytoplasmic protein contents as compared to non-induced MVs. Of note, the endotoxin activity of glycine-induced MVs was approximately eightfold or sixfold lower than that of noninduced MVs when compared at equal protein or lipid concentrations respectively. Nevertheless, glycine-induced MVs efficiently induced both immune responses in a mouse macrophage-like cell line and adjuvanticity in an intranasal vaccine mouse model, comparable to those of non-induced MVs. We propose that the present method of inducing MV production with glycine can be used for emerging biotechnological applications of MVs that have immunomodulatory activities, while dramatically reducing the presence of endotoxins.

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Section: Discussionmentioning

confidence: 99%

Glycine significantly enhances bacterial membrane vesicle production: a powerful approach for isolation of LPS‐reduced membrane vesicles of probiotic Escherichia coli

Hirayama

Nakao

2020

Microbial Biotechnology

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“…The responses elicited by PEG- b -PLGA NPs and PS NPs can be further explained by differences in degradability, antigen/adjuvant persistence, and nanoparticle colloidal stability. These chemical and physical differences would result in downstream differences in lymph node targeting, APC uptake, antigen-processing and presentation [ 36 ]. Hydrophobicity is thought to be a potent danger signal since exposed hydrophobic residues act as universal damage-associated molecular patterns (DAMPs) that normally are only present in necrotic cell death and pathogens [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Hydrophobicity leads to changes in the composition of the protein corona at the injection site and while draining to the lymph node [ 38 ]. The hydrophobic surface of PS NPs and their associated protein corona lead to enhanced interaction with immune cells while potentially diminishing lymph node targeting abilities [ 27 , 30 , 36 , 39 , 40 ]. PEGylated PEG- b -PLGA NPs have enhanced lymphatic drainage and slight diminishment in immune cell uptake [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Biodegradable PLGA-b-PEG Nanoparticles Induce T Helper 2 (Th2) Immune Responses and Sustained Antibody Titers via TLR9 Stimulation

et al. 2020

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Sustained immune responses, particularly antibody responses, are key for protection against many endemic infectious diseases. Antibody responses are often accompanied by T helper (Th) cell immunity. Herein we study small biodegradable poly (ethylene glycol)-b-poly (lactic-co-glycolic acid) nanoparticles (PEG-b-PLGA NPs, 25–50 nm) as antigen- or adjuvant-carriers. The antigen carrier function of PEG-b-PLGA NPs was compared against an experimental benchmark polystyrene nanoparticles (PS NPs, 40–50 nm), both conjugated with the model antigen ovalbumin (OVA-PS NPs, and OVA-PEG-b-PLGA NPs). The OVA-PEG-b-PLGA NPs induced sustained antibody responses to Day 120 after two immunizations. The OVA-PEG-b-PLGA NPs as a self-adjuvanting vaccine further induced IL-4 producing T-helper cells (Th2), but not IFN-γ producing T-cells (Th1). The PEG-b-PLGA NPs as a carrier for CpG adjuvant (CpG-PEG-b-PLGA NPs) were also tested as mix-in vaccine adjuvants comparatively for protein antigens, or for protein-conjugated to PS NPs or to PEG-b-PLGA NPs. While the addition of this adjuvant NP did not further increase T-cell responses, it improved the consistency of antibody responses across all immunization groups. Together these data support further development of PEG-b-PLGA NPs as a vaccine carrier, particularly where it is desired to induce Th2 immunity and achieve sustained antibody titers in the absence of affecting Th1 immunity.

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“…Therefore, to obtain high delivery efficiency, it is necessary to balance the efficiencies of all the steps and ensure that none of them is too low. The tumor’s enhanced permeability and retention (EPR) effect or physical targeting effect greatly promotes the accumulation of nanocarriers at the tumor site [ 28 ]. In addition, some unique characteristics of the TME, such as slight acidity (pH 6.5–7.2), overexpression of proteins and enzymes, and hypoxia, make nanocarriers responsive to internal and external stimuli more effective [ 29 ].…”

Section: Cascade Response Nano-delivery Systemmentioning

confidence: 99%

Application of Nano-Drug Delivery System Based on Cascade Technology in Cancer Treatment

Sun

2021

IJMS

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In the current cancer treatment, various combination therapies have been widely used, such as photodynamic therapy (PDT) combined with chemokinetic therapy (CDT). However, due to the complexity of the tumor microenvironment (TME) and the limitations of treatment, the efficacy of current treatment options for some cancers is unsatisfactory. Nowadays, cascade technology has been used in cancer treatment and achieved good therapeutic effect. Cascade technology based on nanotechnology can trigger cascade reactions under specific tumor conditions to achieve precise positioning and controlled release, or amplify the efficacy of each drug to improve anticancer efficacy and reduce side effects. Compared with the traditional treatment, the application of cascade technology has achieved the controllability, specificity, and effectiveness of cancer treatment. This paper reviews the application of cascade technology in drug delivery, targeting, and release via nano-drug delivery systems in recent years, and introduces their application in reactive oxygen species (ROS)-induced cancer treatment. Finally, we briefly describe the current challenges and prospects of cascade technology in cancer treatment in the future.

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Physical and chemical profiles of nanoparticles for lymphatic targeting

Cited by 92 publications

References 157 publications

Glycine significantly enhances bacterial membrane vesicle production: a powerful approach for isolation of LPS‐reduced membrane vesicles of probiotic Escherichia coli

Glycine significantly enhances bacterial membrane vesicle production: a powerful approach for isolation of LPS‐reduced membrane vesicles of probiotic Escherichia coli

Biodegradable PLGA-b-PEG Nanoparticles Induce T Helper 2 (Th2) Immune Responses and Sustained Antibody Titers via TLR9 Stimulation

Application of Nano-Drug Delivery System Based on Cascade Technology in Cancer Treatment

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