Physical and Chemical Mutagenesis Methods for Development of Insect-Resistant Crop Varieties

Saravanan, K.; Sabesan, T.

doi:10.1007/978-981-13-2652-3_30

Cited by 4 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The rate of spontaneous mutation is relatively very low i.e. 10 −6 or one out of a million for an individual gene [88], therefore, artificial mutations are necessary to increase the percentage of genetic diversity. The process of inducing desirable mutations and exploiting them for crop improvement is called mutation breeding, which comprises three main steps; using mutagens, screening of mutant candidates for desirable traits, and official release of the new variety [89].…”

Section: Mutationmentioning

confidence: 99%

Development of Better Wheat Plants for Climate Change Conditions

Akram¹,

Ghaffar²,

Wadood³

et al. 2022

Wheat

View full text Add to dashboard Cite

Wheat is a staple food of about 40% of the world population, and continuous improvement is vital to meet the increasing demands of the world population. Climate change, a serious concern of the present time, could strongly affect the wheat crop. To mitigate the climate change effects on wheat, scientists are developing wheat germplasm tolerant to the number of stresses and for this purpose different strategies have been adopted. In this chapter, the effect of climate change on wheat and strategies to develop a better wheat plant for climate change using advance breeding and molecular techniques have been discussed. Conventional breeding including hybridization, mutation breeding and shuttle breeding are some classical approaches which have led to the development of some high yielding wheat varieties but it’s a time taking task, the advancement in science has opened the new window for making a better crop for changing climate. Recent achievements in genetic engineering are expected to augment conventional breeding to further increase production. Advances in genome sequencing and molecular breeding have increased the rate of gene discovery. The use of advance genomic technique is a key to overcome the food security issue related to climate change.

show abstract

Section: Mutationmentioning

confidence: 99%

Development of Better Wheat Plants for Climate Change Conditions

Akram¹,

Ghaffar²,

Wadood³

et al. 2022

Wheat

View full text Add to dashboard Cite

show abstract

“…Они позволяют с большей вероятностью получить растения, несущие аллели и признаки, представляющие практическую ценность и экономическую значимость (Jain, 2010). Метод индуцированного мутагенеза считается действенным средством генетической реконструкции сельскохозяйственных растений (Raina et al, 2016;Saravanan, Sabesan, 2019).…”

Section: Introductionunclassified

Studies on mutagenic effect of phosphemide in barley

Bome

2022

Trudy po prikladnoy botanike, genetike i selektsii

View full text Add to dashboard Cite

Background. The method of induced mutagenesis is used to temporarily increase the frequency of mutations, allowing a higher probability of obtaining plants with new alleles and traits valuable for breeding. The search for new agents with high mutagenic activity is of particular interest for the development of mutational breeding. A new mutagen for barley is phosphemide.Materials and methods. Field and laboratory studies were conducted in 2016–2018. Three barley genotypes were treated with an aqueous solution of phosphemide at two concentrations: 0.002% and 0.01%, exposure: 3 hours. Experimental data were statistically processed by the analysis of variance (ANOVA) using Statistica 7. The error of the mean (Sx) was calculated for the mean values. Significance of differences (P < 0.05) was determined by Student’s t-test. Mutation frequency (Mf), mutagenic effectiveness (ME), and efficiency (Me) were assessed.Results and conclusion. It was found that the greatest contribution to formation of field seed germination of mutant populations in M1 and M2 generations was made by the mutagenic factor (20,36%) and interaction of the genotype × environment (18,55%) and mutagen × environment factors (14,93%). The concentration of 0.01% was recognized as semi-lethal for two accessions. In the M2 generation, the mutagenic effectiveness of the 0.002% phosphemide concentration exceeded that of 0.01% more than 4 times. The low concentration was more effective for cv. ‘Zernogradsky 813’ (17.43%) and accession Dz02- 129 (12.04%). For C.I. 10995, a higher concentration of phosphemide had greater effect (29.66%) providing a high mutation frequency (9.79%) against a relatively low lethality (33.00%). Nine different types were identified in the mutation spectrum. In M3 generation, distinct inheritance of the changes was confirmed in 46.43% of families. The highest number of families with confirmed changes was recorded for C.I. 10995 in the experiment with a higher concentration.

show abstract