Physical adsorption of protamine for heparin assay using a quartz crystal microbalance and electrochemical impedance spectroscopy

Cheng, Tzong Jih; Lin, Tsung-Han; Chang, Hsien Chang

doi:10.1016/s0003-2670(02)00335-5

Cited by 32 publications

(20 citation statements)

References 24 publications

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“…An irreversible ion-selective polymer membrane electrode was built for the rapid and selective detection of heparin, which was later modified by the addition of a H + ionophore to the electrode membrane to reversibly sense heparin (Ambrose and Meyerhoff, 1997;Ma et al, 1993;Mathison and Bakker, 1999;Shvarev and Bakker, 2003). The quartz crystal microbalance (QCM) has also been suggested to determine the absolute concentration of heparin due to its binding with protamine-adsorbed surface (Cheng et al, 2002), and to investigate the effect of pH on the ion dynamics in composite PPy/heparin films (Hepel et al, 1997). More recently, Guo et al (2005) reported a voltammetric detection of heparin at polarized blood plasma/1,2-dichloroethane interfaces.…”

Section: Introductionmentioning

confidence: 99%

An EQCM study on the interaction of heparin with the charge-transfer complex generated during o-tolidine electrooxidation: A biosensing mode with a dynamically renewed surface

Cao

Jiang

Meng

et al. 2007

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

An EQCM study on the interaction of heparin with the charge-transfer complex generated during o-tolidine electrooxidation: A biosensing mode with a dynamically renewed surface

Cao

Jiang

Meng

et al. 2007

Biosensors and Bioelectronics

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“…Various approaches such as quartz crystal microbalance, surface plasmon resonance, ion-sensitive fieldeffect transistor, and membrane-based ion-selective electrode involve either protamine or synthetic cationic polymers as heparin probes, and they are based on either surface affinity capture or an automated heparin titration (23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). Such methods have had variable success in fully achieving the required objectives: selectivity, sensitivity, robustness, and reusability.…”

mentioning

confidence: 99%

Monitoring of heparin and its low-molecular-weight analogs by silicon field effect

Milović

Behr

Godin

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Heparin is a highly sulfated glycosaminoglycan that is used as an important clinical anticoagulant. Monitoring and control of the heparin level in a patient's blood during and after surgery is essential, but current clinical methods are limited to indirect and off-line assays. We have developed a silicon field-effect sensor for direct detection of heparin by its intrinsic negative charge. The sensor consists of a simple microfabricated electrolyte-insulatorsilicon structure encapsulated within microfluidic channels. As heparin-specific surface probes the clinical heparin antagonist protamine or the physiological partner antithrombin III were used. The dose-response curves in 10% PBS revealed a detection limit of 0.001 units͞ml, which is orders of magnitude lower than clinically relevant concentrations. We also detected heparin-based drugs such as the low-molecular-weight heparin enoxaparin (Lovenox) and the synthetic pentasaccharide heparin analog fondaparinux (Arixtra), which cannot be monitored by the existing near-patient clinical methods. We demonstrated the specificity of the antithrombin III functionalized sensor for the physiologically active pentasaccharide sequence. As a validation, we showed correlation of our measurements to those from a colorimetric assay for heparin-mediated anti-Xa activity. These results demonstrate that silicon field-effect sensors could be used in the clinic for routine monitoring and maintenance of therapeutic levels of heparin and heparin-based drugs and in the laboratory for quantitation of total amount and specific epitopes of heparin and other glycosaminoglycans.heparin sensors ͉ label-free sensing ͉ medical devices ͉ enoxaparin ͉ fondaparinux

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“…(Casey 2004). Despite a wider application of enzymatic methods of analysis in laboratory diagnostics of cardio-pathology, several works of the recent years have described piezoelectric chemical, bio-and immunosensors for determining biomarkers of non-enzymatic nature -myoglobin (Godber et al 2005), C-reactive protein ( RP) (Kurosawa et al 2003, Kim et al 2009), troponin (Wong-ek et al 2010, Mohammed andDesmulliez 2011), heparin (Cheng et al 2002), thrombin, etc.…”

Section: Cardiac Markersmentioning

confidence: 99%

Capabilities of Piezoelectric Immunosensors for Detecting Infections and for Early Clinical Diagnostics

Ermolaeva¹,

Kalmykova²

2012

Advances in Immunoassay Technology

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Physical adsorption of protamine for heparin assay using a quartz crystal microbalance and electrochemical impedance spectroscopy

Cited by 32 publications

References 24 publications

An EQCM study on the interaction of heparin with the charge-transfer complex generated during o-tolidine electrooxidation: A biosensing mode with a dynamically renewed surface

An EQCM study on the interaction of heparin with the charge-transfer complex generated during o-tolidine electrooxidation: A biosensing mode with a dynamically renewed surface

Monitoring of heparin and its low-molecular-weight analogs by silicon field effect

Capabilities of Piezoelectric Immunosensors for Detecting Infections and for Early Clinical Diagnostics

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