Physical Activity Interferes with the Immunomodulatory Effect of the Antineoplastic Drug NSC631570

Skivka, Larysa; Fedorchuk, O. G.; Susak, Yaroslav; Susak, Mychailo Y.; Malanchuk, O. M.; Rudyk, Mariia; Nowicky, Yaroslav. W.

doi:10.2174/1389201015666141113125358

Cited by 7 publications

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“…Neutrophils produce active forms of oxygen, which contributes to the development of inflammation (Fernández-Sánchez et al, 2011;Lutai et al, 2016). The functional polarization of phagocytes (neutrophils and monocytes), characterized by the ratio of the production of reactive forms of oxygen and phagocytic activity, depends on many factors, in particular, the period of the day, physical activity, the state of lipid metabolism (Skivka et al, 2015a(Skivka et al, , 2015bRudyk et al, 2016Rudyk et al, , 2018. There are data on dependence of functional status of neutrophils on thyroid status parameters (Mezosi et al, 2005;Marino et al, 2006;Mathison, 2006;Kurjane et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Interdependence of oxidative/antioxidant system indicators and thyroid status under conditions of prolonged exposure to small doses of radiation

Sokolenko¹,

Sokolenko²

2019

Regul. Mech. Biosyst.

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We have studied the interdependence of the intensity of oxidative processes/antioxidant level and the thyroid status parameters in a group of students aged 18–24 who lived for a long time in the territory of enhanced radioecological control (density of soil contamination by isotopes (137Cs 3.7 ∙ 104 – 18.5 ∙ 104 Bq/m2). We examined 50 people from relatively environmentally friendly areas (control group) and 50 people from IV radiation zone (experimental group). In the experimental group, there were no individuals with clinical manifestations of thyroid pathology. However, subgroups with signs of hyperthyroidism and hypothyroidism were identified. We evaluated the level of cortisol, thyrotrophic hormone (TSH), triiodothyronine (T3), thyroxine (T4), malonic dialdehyde (MDA), ceruloplasmin (CP), transferrin (Tf), sulfhydryl groups (SH); we calculated the oxidative stress index (OSI). The research was conducted one month before the examination time and also during the exams as a factor in increased emotional stress. A lowered CP level was found in the subgroup with signs of hypothyroidism; SH groups – in all subgroups, separated by thyroid status. The oxidative stress index was higher in all students examined of the experimental group, compared with the control. The growth of MDA level is marked in the experimental group – it is the most strongly pronounced in conditions of additional emotional load in people with signs of hyperthyroidism and hypothyroidism. CP level significantly decreased in the subgroup of hyperthyroidism on the background of T3 decrease. OSI increased in all students examined from the experimental group. In the subgroup of hypothyroidism it became significantly higher than in the subgroup of euthyroidism. A positive correlation between the levels of CP and T3 was found. The highest values of the correlation coefficients were noted for subgroups with signs of hyperthyroidism and hypothyroidism, with the coefficient significance increasing under conditions of emotional stress. The index of oxidative stress in the experimental group positively correlated with the level of TSH – in terms of emotional stress, the statistical significance of the coefficients disappeared. In the subgroups divided by thyroid status, variability of interactions between OSI and T3 was observed but it was not statistically significant. It was found that the participation of thyroid status in supporting redox homeostasis in people aged 18–24 who suffered from chronic small-doze radiation exposure was realized mainly by the influence on the antioxidant system. The ability of thyroid hormones to maintain a proper antioxidant state was suppressed in this group. The unbalanced relationship between thyroid hormones and oxidative stress indicators is strongly manifested under conditions of additional emotional stress.

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Section: Discussionmentioning

confidence: 99%

Interdependence of oxidative/antioxidant system indicators and thyroid status under conditions of prolonged exposure to small doses of radiation

Sokolenko¹,

Sokolenko²

2019

Regul. Mech. Biosyst.

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“…Granulocytes or monocytes were gated according to forward and side scatters. Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) was used to evaluate nonspecific reactivity reserve in phagocytes (Shapiro et al 2011 ; Skivka et al 2015 ). Reactivity reserve was characterized by the modulation coefficient (MC) that was calculated by the following formula: where S is the ROS value in probes stimulated with PMA in vitro, and B is ROS value in unstimulated probes (basal value).…”

Section: Methodsmentioning

confidence: 99%

C60 fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes

et al. 2018

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BackgroundC60 fullerene-based nanoformulations are proposed to have a direct toxic effect on tumor cells. Previous investigations demonstrated that C60 fullerene used alone or being conjugated with chemotherapeutic agents possesses a potent anticancer activity. The main aim of this study was to investigate the effect of C60 fullerene and its nanocomplexes with anticancer drugs on human phagocyte metabolic profile in vitro.MethodsAnalysis of the metabolic profile of phagocytes exposed to C60 fullerene in vitro revealed augmented phagocytic activity and down-regulated reactive nitrogen species generation in these cells. Additionally, cytofluorimetric analysis showed that C60 fullerene can exert direct cytotoxic effect on normal and transformed phagocytes through the vigorous induction of intracellular reactive oxygen species generation.ResultsCytotoxic action as well as the pro-oxidant effect of C60 fullerene was more pronounced toward malignant phagocytes. At the same time, C60 fullerenes have the ability to down-regulate the pro-oxidant effect of cisplatin on normal cells. These results indicate that C60 fullerenes may influence phagocyte metabolism and have both pro-oxidant and antioxidant properties.ConclusionsThe antineoplastic effect of C60 fullerene has been observed by direct toxic effect on tumor cells, as well as through the modulation of the functions of effector cells of antitumor immunity.

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“…The number of monocytes in peripheral blood does not change significantly with age, age-related changes are mainly related to macrophage activity, however, there is evidence of phagocytosis inhibition in monocytes and the production of reactive oxygen (Plowden et al, 2004;Hearps et al, 2012;Metcalf et al, 2015). In general, when exposed to extreme exogenous factors, phagocytic activity is indicated among the sensitive factors of innate immunity (Skivka et al, 2015a;Boieva et al, 2018). The age-related changes in the number and function of NK cells, which are considered to be short-lived effector cells, are quite controversial.…”

Section: Discussionmentioning

confidence: 99%

Manifestations of allostatic load in residents of radiation contaminated areas aged 18–24 years

Sokolenko¹,

Sokolenko²

2019

Regul. Mech. Biosyst.

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We studied the features of allostatic load (AL) in 100 students aged 18–24 years old who, from birth to adulthood, lived in the territories assigned to the IV radiation zone after the Chornobyl accident (density of soil contamination by isotopes 137Cs 3.7–18.5∙104 Bq/m2) and underwent prolonged exposure to small doses of ionizing radiation. The examined students did not have any clinical signs of the immune-neuroendocrine system dysfunction. 50 people had signs of vegetative-vascular dystonia syndrome (VVD), 48 had signs of moderate hyperthyroidism and 21 had signs of moderate hypothyroidism. During the examination session, as a factor of additional psycho-emotional load, in 66 of the examined the immunoregulatory index CD4+/CD8+ went below the lower limit of the homeostatic norm, in 62 of the examined low density lipoprotein cholesterol (LDL-C) exceeded the upper level. The relative risk (RR) and attributable risk (AR) of the participation of potential secondary factors of allostatic load formation in CD4+/CD8+ immunoregulatory index going below the lower limit were calculated. The presence of statistically significant relative risk of participation in the formation of suppression of the index CD4+/CD8+: the state of hyperthyroidism, state of hypothyroidism, vegetative-vascular dystonia syndrome, higher than normal LDL-C. When the examined students combined the signs of hyperthyroidism, vegetative-vascular dystonia syndrome and higher level of LDL-C; with combination of signs of hypothyroidism, vegetative-vascular dystonia syndrome and higher level of LDL-C. The attributable risk in all cases exceeded 0.10, which confirmed the importance of some of these factors and their complexes in the formation of the effect of reduced immunoregulatory index. The CD4+/CD8+ index can be considered an important biomarker of AL and premature age-related changes in the immune system in residents of radiation-contaminated areas. The risk of AL formation in the case of occurrence of a complex of mediated secondary biomarkers (vegetative-vascular dystonia syndrome, thyroid dysfunction, hypercholesterolemia) is higher compared to their individual significance.

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Physical Activity Interferes with the Immunomodulatory Effect of the Antineoplastic Drug NSC631570

Cited by 7 publications

References 0 publications

Interdependence of oxidative/antioxidant system indicators and thyroid status under conditions of prolonged exposure to small doses of radiation

Interdependence of oxidative/antioxidant system indicators and thyroid status under conditions of prolonged exposure to small doses of radiation

C60 fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes

Manifestations of allostatic load in residents of radiation contaminated areas aged 18–24 years

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