Phylogeographical Features of<i>Octopus vulgaris</i>and<i>Octopus insularis</i>in the Southeastern Atlantic Based on the Analysis of Mitochondrial Markers

Sales, João Bráullio de Luna; Rêgo, Péricles Sena do; Hilsdorf, Alexandre Wagner Silva; Moreira, Angela Aparecida; Haimovici, Manuel; Tomás, Acácio Ribeiro Gomes; Batista, Bruno Braulino; Marinho, Reynaldo Amorim; Markaida, Unai; Schneider, Horácio; Sampaio, Iracilda

doi:10.2983/035.032.0211

Cited by 27 publications

(27 citation statements)

References 24 publications

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“…Cycling conditions for CO1 are 35 cycles of 10 sec denaturing at 98°C, 15 sec annealing at 48°C, and 10 sec elongation at 72°C. Primers for 16S were described previously (Sales et al 2013b) L1987 GCCTCGCCTG TTTACCAAAAAC (forward), H2609 CGGTCTGAACTCA GATCACGT (reverse). 16S amplifications were carried out in 25-lL reactions containing 2.5 lL 109 PCR buffer (100 mmol/L Tris-HCl pH 9, 500 mmol/L KCl, 15 mmol/L MgCl 2 , 1% Triton X-100), 18.5 lL water, 10 mmol/L dNTPs, 1.0 lg each primer, 1 unit taq polymerase, and 25-50 ng total DNA.…”

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

A revisited phylogeography of Nautilus pompilius

Vandepas

Dooley

Barord

et al. 2016

Ecology and Evolution

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The cephalopod genus Nautilus is considered a “living fossil” with a contested number of extant and extinct species, and a benthic lifestyle that limits movement of animals between isolated seamounts and landmasses in the Indo‐Pacific. Nautiluses are fished for their shells, most heavily in the Philippines, and these fisheries have little monitoring or regulation. Here, we evaluate the hypothesis that multiple species of Nautilus (e.g., N. belauensis, N. repertus and N. stenomphalus) are in fact one species with a diverse phenotypic and geologic range. Using mitochondrial markers, we show that nautiluses from the Philippines, eastern Australia (Great Barrier Reef), Vanuatu, American Samoa, and Fiji fall into distinct geographical clades. For phylogenetic analysis of species complexes across the range of nautilus, we included sequences of Nautilus pompilius and other Nautilus species from GenBank from localities sampled in this study and others. We found that specimens from Western Australia cluster with samples from the Philippines, suggesting that interbreeding may be occurring between those locations, or that there is limited genetic drift due to large effective population sizes. Intriguingly, our data also show that nautilus identified in other studies as N. belauensis, N. stenomphalus, or N. repertus are likely N. pompilius displaying a diversity of morphological characters, suggesting that there is significant phenotypic plasticity within N. pompilius.

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Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

A revisited phylogeography of Nautilus pompilius

Vandepas

Dooley

Barord

et al. 2016

Ecology and Evolution

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“…The mean COX1 divergence between haplogroups (1.0%) is an overestimate because it is calculated on sequence data from selected specimens harboring different CR haplotypes. The highest COX1 p-distance, 1.6%, is unremarkable (typical of intraspecific values), lower than the estimate between eastern and western Atlantic O. vulgaris (2.6%) of Sales et al [50], and similar to the maximum estimated divergence value (1.3%) for South African populations [28]. In contrast, the mean value between two haplogroups for Enteroctopus dofleini Wülker, 1910, 2.9%, suggests cryptic lineage speciation [53].…”

Section: Genetic Diversitymentioning

confidence: 57%

“…For example, samples from Galicia (h = 0.786, π = 0.0018) and Pico (h = 0.653, π = 0.0097) are similar to values for A. dux, indicative of exceptionally low global genetic diversity [49]. For COX1 data, the nucleotide diversity (π) value of 0.0063 is similar to that for O. vulgaris (0.004) in the Atlantic [50]. Population-level values for h and π are consistent with general patterns for animal COX1 diversity (h = 0.7013, π = 0.0036) [51], but higher than values reported for other cephalopods [52].…”

Section: Genetic Diversitymentioning

confidence: 75%

Phylogeography of the insular populations of common octopus, Octopus vulgaris Cuvier, 1797, in the Atlantic Macaronesia

et al. 2020

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Exploited, understudied populations of the common octopus, Octopus vulgaris Cuvier, 1797, occur in the northeastern Atlantic (NEA) throughout Macaronesia, comprising the Azores, Madeira and Canaries, and also the Cabo Verde archipelago. This octopus species, found from the intertidal to shallow continental-shelf waters, is largely sedentary, and the subject of intense, frequently unregulated fishing effort. We infer connectivity among insular populations of this octopus. Mitochondrial control region and COX1 sequence datasets reveal two highly divergent haplogroups (α and β) at similar frequencies, with opposing clinal distributions along the sampled latitudinal range. Haplogroups have different demographic and phylogeographic patterns, with origins related to the two last glacial maxima. F ST values suggest a significant differentiation for most pairwise comparisons, including insular and continental samples, from the Galicia and Morocco coasts, with the exception of pairwise comparisons for samples from Madeira and the Canaries populations. Results indicate the existence of genetically differentiated octopus populations throughout the NEA. This emphasizes the importance of regulations by autonomous regional governments of the Azores, Madeira and the Canaries, for appropriate management of insular octopus stocks.

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“…Our phylogenetic reconstruction also showed overall improvements in support values throughout the tree when compared to previous mtDNA-based topologies. In particular, previous mtDNA-based studies found poor support for the clade containing O. vulgaris Type II individuals 29,41 . Datasets with multiple unlinked nuclear DNA loci have a greater ability to resolve phylogenetic relationships in comparison to mtDNA alone 10,11 .…”

Section: Discussionmentioning

confidence: 87%

Genome-wide sequencing uncovers cryptic diversity and mito-nuclear discordance in theOctopus vulgarisspecies complex

Amor

Doyle

Norman

et al. 2019

Preprint

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Many marine species once considered to be cosmopolitan are now recognised as cryptic species complexes. Mitochondrial markers are ubiquitously used to address phylogeographic questions, and have been used to identify some cryptic species complexes; however, their efficacy in inference of evolutionary processes in the nuclear genome has not been thoroughly investigated. We used double digest restriction siteassociated DNA sequencing (ddRADseq) markers to quantify species boundaries in the widely distributed and high value common octopus, Octopus vulgaris, comparing genome-wide phylogenetic signal to that obtained from mitochondrial markers.Phylogenetic analyses, genome-wide concordance and species tree estimation based on 604 genome-wide ddRADseq loci revealed six species within the O. vulgaris group.Divergence time estimates suggested modern-day species evolved over the last 2.5 ma, during a period of global cooling. Importantly, our study identified significant phylogenetic discordance between mitochondrial and nuclear markers; genome-wide nuclear loci supported O. vulgaris sensu stricto and Type III (South Africa) as distinct species, which mtDNA failed to recognise. Our finding of conflicting phylogenetic signal between mitochondrial and nuclear markers has broad implications for many taxa.Improved phylogenetic resolution of O. vulgaris has significant implications for appropriate management of the group and will allow greater accuracy in global fisheries catch statistics.

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Phylogeographical Features ofOctopus vulgarisandOctopus insularisin the Southeastern Atlantic Based on the Analysis of Mitochondrial Markers

Cited by 27 publications

References 24 publications

A revisited phylogeography of Nautilus pompilius

A revisited phylogeography of Nautilus pompilius

Phylogeography of the insular populations of common octopus, Octopus vulgaris Cuvier, 1797, in the Atlantic Macaronesia

Genome-wide sequencing uncovers cryptic diversity and mito-nuclear discordance in theOctopus vulgarisspecies complex

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