Phylogeny of the Whipple's-disease-associated bacterium

Wilson, Kenneth H.; Frothingham, Richard; Wilson, Joanne A. P.; Blitchington, R B

doi:10.1016/0140-6736(91)90545-z

Cited by 315 publications

(150 citation statements)

References 4 publications

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“…However, infection with these bacilli has yet to be documented in a patient with AIDS. Wilson (642). Recently, Relman and colleagues reported a technique in which oligonucleotide primers specific for the Whipple's disease bacillus were used in a PCR to amplify sequences of bacterial 16S rRNA directly from infected tissues (469).…”

Section: Taxonomy and Microbiologic Aspectsmentioning

confidence: 99%

The medically important aerobic actinomycetes: epidemiology and microbiology

1994

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The aerobic actinomycetes are soil-inhabiting microorganisms that occur worldwide. In 1888, Nocard first recognized the pathogenic potential of this group of microorganisms. Since then, several aerobic actinomycetes have been a major source of interest for the commercial drug industry and have proved to be extremely useful microorganisms for producing novel antimicrobial agents. They have also been well known as potential veterinary pathogens affecting many different animal species. The medically important aerobic actinomycetes may cause significant morbidity and mortality, in particular in highly susceptible severely immunocompromised patients, including transplant recipients and patients infected with human immunodeficiency virus. However, the diagnosis of these infections may be difficult, and effective antimicrobial therapy may be complicated by antimicrobial resistance. The taxonomy of these microorganisms has been problematic. In recent revisions of their classification, new pathogenic species have been recognized. The development of additional and more reliable diagnostic tests and of a standardized method for antimicrobial susceptibility testing and the application of molecular techniques for the diagnosis and subtyping of these microorganisms are needed to better diagnose and treat infected patients and to identify effective control measures for these unusual pathogens. We review the epidemiology and microbiology of the major medically important aerobic actinomycetes.

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Section: Taxonomy and Microbiologic Aspectsmentioning

confidence: 99%

The medically important aerobic actinomycetes: epidemiology and microbiology

1994

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“…Comparative sequence analysis of the 16s rRNA gene (I 6 s rDNA) allowed the hitherto uncultivated presumptive agent of Whipple's disease to be identified as an actinomycete bacterium constituting the novel but not yet validated taxon ' Tropheryma whippelii' (Wilson et al, 1991 ;Relman et al, 1992). Further, the sequence of the intergenic spacer region between the 16s and the 23s rDNA as well as the 5' end of the 23s rDNA have also been determined (Maiwald et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Detection of three different types of ‘Tropheryma whippelii’ directly from clinical specimens by sequencing, single-strand conformation polymorphism (SSCP) analysis and type-specific PCR of their 16S-23S ribosomal intergenic spacer region

Hinrikson

Dutly

Nair

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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The 165-235 rDNA intergenic spacer region of organisms identical with or closely related to ' Tropheryma whippelii', the uncultivated causative agent of Whipple's disease, was analysed directly from 38 clinical specimens of 28 patients using a specific nested PCR followed by direct sequencing. As compared to the reference sequence in public databases, two novel ' T. whippelii' spacer types were recognized. In the absence of DNA-DNA hybridization data it is uncertain whether the three types found represent subtypes of a single species or three different but closely related species. Methods were developed to detect all three variants by single-strand conformation polymorphism analysis and by type-specif ic PCR assays, thus allowing the screening of large numbers of specimens. Further studies may provide a clue to the possible associations between the type of infecting strain and the various clinical presentations of Whipple's disease.

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“…Using this approach, the causative bacteria for bacillary angiomatosis and Whipple's disease have been identified. 45,46 In both diseases, bacteria could be seen in histologic sections, but could not be grown in culture. Attempts to amplify bacterial DNA from sarcoidosis specimens have been less encouraging.…”

Section: Novel Nonculturable Microbesmentioning

confidence: 99%

Applications of the Polymerase Chain Reaction to Infectious Disease Diagnosis

Frothingham

1996

Ann Saudi Med

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Phylogeny of the Whipple's-disease-associated bacterium

Cited by 315 publications

References 4 publications

The medically important aerobic actinomycetes: epidemiology and microbiology

The medically important aerobic actinomycetes: epidemiology and microbiology

Detection of three different types of ‘Tropheryma whippelii’ directly from clinical specimens by sequencing, single-strand conformation polymorphism (SSCP) analysis and type-specific PCR of their 16S-23S ribosomal intergenic spacer region

Applications of the Polymerase Chain Reaction to Infectious Disease Diagnosis

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