Phylogeny of Leptographium qinlingensis cytochrome P450 genes and transcription levels of six CYPs in response to different nutrition media or terpenoids

Dai, Lulu; Zheng, Jie; Ye, Jiaqi; Chen, Hui

doi:10.1007/s00203-021-02616-9

Cited by 5 publications

(8 citation statements)

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“…In order to explore the effects of different carbon source treatments on the physiological phenotype and TOR gene expression of L. qinlingensis, 8 media were set up as follows [ 24 , 26 ]: CK: 1% glucose, 0.67% amino free yeast, pH = 5.5; Processing group: replaced glucose with sucrose/maltose/fructose/lactose/amylum/sorbitol/mannitol, other conditions were the same.…”

Section: Methodsmentioning

confidence: 99%

“…In order to investigate the effects of different nitrogen sources on the physiological phenotype and TOR gene expression of L. qinlingensis, 16 media were set up (6 inorganic nitrogen sources, 4 organic nitrogen sources and 2 host nutrients) as follows [ 24 , 26 , 27 ]: CK: 0.3% NH 4 NO 3 , 0.17% amino free yeast, 0.1% potassium hydrogen phthalate, 1% maltose, pH = 5.5; processing group: replaced NH 4 NO 3 with (NH 4 ) 2 C 2 O 4 /NH 4 Cl/(NH 4 ) 2 HPO 4 /(NH 4 ) 2 SO 4 /(CO(NH 2 ) 2 ), other conditions were the same.…”

Section: Methodsmentioning

confidence: 99%

“…Different terpenoids (concentrations of 5%, 10%, and 20% for each terpenoid treatment) were prepared in different medium formulations as follows [ 24 , 26 ]: CK: 5% DMSO, 0.83% maltose, 0.75% agar, pH = 5.5; processing group: replace DMSO with (±)-α-pinene/(−)-β-pinene/(+)-3-carene/(+)-limonene/turpentine/mix-monoterpene, other conditions were the same.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, three toxins (6-methoxymethyleugenin, maculosin and cerevisterol) synthesized by L. qinlingensis are phytotoxic to the P. armandii seedlings [ 13 ]. In addition, inoculation with L. qinlingensis increases the concentrations of monoterpenes and sesquiterpenes in the phloem and xylem of the P. armandii seedlings [ 22 , 23 , 24 ]. These terpenoids play an important role in the settlement of D. armandi on P. armandii .…”

Section: Introductionmentioning

confidence: 99%

“…G . clavigera can survive independently and is induced by exposure to the lodgepole pine phloem extract (LPPE) [ 26 ] or oleoresin terpenoids [ 24 , 27 ]. Therefore, L. qinlingensis and G .…”

Section: Introductionmentioning

confidence: 99%

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Molecular Mechanism of Overcoming Host Resistance by the Target of Rapamycin Gene in Leptographium qinlingensis

Gan

Tang

et al. 2022

Microorganisms

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Leptographium qinlingensis is a fungal symbiont of the Chinese white pine beetle (Dendroctonus armandi) and a pathogen of the Chinese white pine (Pinus armandii) that must overcome the terpenoid oleoresin defenses of host trees to invade and colonize. L. qinlingensis responds to monoterpene flow with abundant mechanisms that include the decomposing and use of these compounds as a nitrogen source. Target of Rapamycin (TOR) is an evolutionarily conserved protein kinase that plays a central role in both plants and animals through integration of nutrients, energies, hormones, growth factors and environmental inputs to control proliferation, growth and metabolism in diverse multicellular organisms. In this study, in order to explore the relationship between TOR gene and carbon sources, nitrogen sources, host nutrients and host volatiles (monoterpenoids) in L. qinlingensis, we set up eight carbon source treatments, ten nitrogen source treatments, two host nutrients and six monoterpenoids (5%, 10% and 20%) treatments, and prepared different media conditions. By measuring the biomass and growth rate of mycelium, the results revealed that, on the whole, the response of L. qinlingensis to nitrogen sources was better than carbon sources, and the fungus grew well in maltose (carbon source), (NH4)2C2O4 (inorganic nitrogen source), asparagine (organic nitrogen source) and P. armandii (host nutrient) versus other treatments. Then, by analyzing the relationship between TOR expression and different nutrients, the data showed that: (i) TOR expression exhibited negative regulation in response to carbon sources and host nutrition. (ii) The treatments of nitrogen sources and terpenoids had positively regulatory effects on TOR gene; moreover, the fungus was most sensitive to β-pinene and 3-carene. In conclusion, our findings reveal that TOR in L. qinlingensis plays a key role in the utilization of host volatiles as nutrient intake, overcoming the physical and chemical host resistances and successful colonization.

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Section: Methodsmentioning

confidence: 99%