Phylogeny of five predominant pospiviroid species in Belgium

Bogaert, Noémi Van; Smagghe, Guy; Maes, Martine; Backer, Mathias De; Jonghe, Kris De

doi:10.1007/s10658-017-1158-0

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…In this way, the number of reports describing its presence in tomato and other natural hosts, frequently asymptomatic ornamental plants, has dramatically raised in the last years Ling & Zhang, 2009;Candresse et al, 2010;Fox et al, 2013;Shiraishi et al, 2013;Van Bogaert et al, 2017;Verhoeven et al, 2017). In this work, we have reported for the first time the natural infection of eggplant plants by TCDVd.…”

Section: Discussionmentioning

confidence: 79%

“…TCDVd is a pospiviroid highly related to PSTVd (Singh et al, 1999;Di Serio et al, 2014) of increasing concern, as it causes important damages in the tomato crops (Singh et al, 1999;Candresse et al, 2010;Singh et al, 2010;Nie, 2012;Fox et al, 2013;Van Bogaert et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Plantlets and adult plants of eggplant testing positive for TCDVd, as well as siblings testing negative, were examined for symptoms of infection described in tomato, such as plant dwarfism, growth reduction, top bunching, epinasty, bronzing, leaf (in particular young ones) yellowing and curling, small and narrow leaves, and small and deformed fruits (Singh et al, 1999;Candresse et al, 2010;Singh et al, 2010;Nie, 2012;Fox et al, 2013;Van Bogaert et al, 2017). Negative testing siblings were used as controls and also examined for the same symptoms.…”

Section: Evaluation Of Symptomsmentioning

confidence: 99%

“…In tomato, TCDVd may cause severe symptoms including plant dwarfism, top bunching, epinasty, leaf yellowing and size reduction, and fruit size reduction and deformation (Candresse et al, 2010;Fox et al, 2013;Van Bogaert et al, 2017). TCDVd, as other pospiviroids, has been frequently found to be naturally present in ornamental plants (James et al, 2008;Cervena et al, 2013;Candresse et al, 2017;Van Bogaert et al, 2017), which generally are asymptomatic (Shiraishi et al, 2013). TCDVd is known to be mechanically transmitted with contaminated tools (Matsushita et al, 2009;Matsuura et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

“…Differences in nucleotide sequence can affect pathogenicity, transmission, and host range of viroids (Wasseneger et al, 1996;Hadidi et al, 2003). In this respect, differences in the sequence of TCDVd isolates have been reported (Van Bogaert et al, 2017), which may account for differences in aggressiveness (Kovalskaya & Hammond, 2014;Škorić, 2017;Yanagisawa et al, 2019). Eggplant (Solanum melongena L.) is a natural host for several viroids such as PSTVd, which causes stunted growth (Minoia et al, 2015), citrus exocortis viroid (CEVd), or eggplant latent viroid (ELVd), although plants infected with these two latter viroids remain asymptomatic (Fagoaga & Durán-Vila, 1996;Darós, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Detection, molecular characterisation and aspects involving the transmission of tomato chlorotic dwarf viroid in eggplant

Gramazio

Lerma

Villanueva

et al. 2019

Annals of Applied Biology

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Tomato chlorotic dwarf viroid (TCDVd) is a pospiviroid that causes severe disease symptoms in tomato. TCDVd is also naturally found in other crops and plants, in most occasions being asymptomatic. Apart from the natural hosts reported up to now, artificial inoculations have revealed that TCDVd can infect other plants, including eggplant (Solanum melongena). In a screening of seedlings of eggplant from a breeding programme we detected a pospiviroid, which we identified as TCDVd, representing the first report of natural infection of eggplant by TCDVd. The new TCDVd isolate of eggplant was detected by reverse transcription polymerase chain reaction (RT‐PCR) using primers TG21/CT20, initially designed to detect potato spindle tuber viroid. The new isolate sequence is close to a Brugmansia sanguinea isolate of TCDVd from the Netherlands, and most of the nucleotidic changes with respect to this isolate and to the reference genome sequence of TCDVd are found in the TR region. Naturally infected plants of eggplant with this TCDVd isolate did not display any disease symptoms. We demonstrated that in eggplant TCDVd is mechanically transmitted with low to moderate efficiency with cultivation practices, but not by plant‐to‐plant contact. Tomato plants artificially inoculated with the eggplant isolate of TCDVd tested positive for the presence of the viroid at 50 days after inoculation, but did not display any disease symptoms. Seed transmission to germinated seedlings of eggplant was variable among progenies from infected plants, ranging from 7.7% to 100.0%. Disinfection of seeds with chemical treatments with sodium hypochlorite and trisodium phosphate solutions plus thermotherapy at 80°C for 24 hr or 90°C for 6 hr was ineffective in reducing the rate of transmission by seed. We did not find evidence of horizontal transmission of TCDVd by pollen, but vertical transmission was highly efficient when healthy eggplant plants were pollinated with infected pollen. Our results indicate that asymptomatic infection of eggplant by TCDVd and high seed and pollen transmission rates may contribute to the spread of this viroid. The information we obtained is useful in order to implement measures for the prevention, control and eradication of TCDVd in eggplant crops, as well as to avoid their transmission to other hosts.

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