Phylogeny, morphology and pathogenicity of Diaporthe and Phomopsis species on almond in Portugal

Grapevine trunk diseases (GTD) have a variety of symptoms and causes. The latter include fungal species from the family Diaporthaceae. The aim of our study was to determine Diaporthaceae species present in the woody parts of grapevines sampled from 12 vine-growing coastal and continental areas of Croatia. The fungi were isolated from diseased wood, and cultures analysed for phenotype (morphology and pathogenicity) and DNA sequence (ITS1, 5.8S, ITS2). Most isolates were identifi ed as Phomopsis viticola, followed by Diaporthe neotheicola and Diaporthe eres. This is the fi rst report of Diaporthe eres as a pathogen on grapevine in the world, while for Diaporthe neotheicola this is the fi rst report in Croatia. Pathogenicity trials confi rmed Phomopsis viticola as a strong and Diaporthe neotheicola as a weak pathogen. Diaporthe eres turned out to be a moderate pathogen, which implies that the species could have a more important role in the aetiology of GTD.

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“…in Portugal (28). Our study has demonstrated weak pathogenicity of D. neotheicola, which is supported by previous studies in the world (38).…”

Section: Discussionsupporting

confidence: 92%

“…Beside grapevine, this fungal species has been reported on tea plant (Camelia sinensis (L.) Kuntze) in Italy (37) and as a weak pathogen on almond (Prunus dulcis (Mill.) D.A.Webb) in Portugal (38) and fennel (Foeniculum vulgare Mill.) in Portugal (28).…”

Section: Discussionmentioning

confidence: 99%

Grapevine Trunk Diseases Associated with Fungi from the Diaporthaceae Family in Croatian Vineyards / Identifikacija Vrsta Roda Fusarium Izoliranih S Plodova Jabuke Nakon Skladištenja

Kaliterna

Miličević

Cvjetković

2012

Archives of Industrial Hygiene and Toxicology

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“…The species grouping based on ITS sequences obtained by Diogo et al (2010) and Santos and Phillips (2009) was consistent with the groups revealed using the ISSR-PCR assay. In our study, the ISSR-PCR and RAPD assays provided additional confirmation of genetic distance existing between D. vaccinii and another closely related Diaporthe species (occurring on cranberry) and justifies their affiliation to distinct species.…”

Section: Discussionsupporting

confidence: 82%

“…The ITS region of ribosomal gene clusters, although commonly used to infer phylogenies in fungi (Nilsson et al 2008), was recently demonstrated to show high within-species variation in some Diaporthe/Phomopsis species . However, in the study performed by Diogo et al (2010) it was still possible to distinguish species in this genus using ITS data, due to little species variation of P. amygdali. The phylogenetic analysis performed by Udayanga and others (2014) showed that, beside several other analysed DNA regions, clear (99-100% bootstrap value) distinction of D. vaccinii strain CBS 160.32 from the closely related D. alleghaniensis strain, based on rDNA ITS sequence only.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of Diaporthe vaccinii Shear causing upright dieback and viscid rot of cranberry in Poland

2016

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During the summers of 2013-2014, symptoms similar to viscid rot and upright disease were observed on cranberries (Vaccinium macrocarpon) on one plantation in central Poland. The associated fungi were isolated from symptomatic plant tissue. On the basis of morphological and cultural characteristics and the ability of isolated fungi to elicit viscid rot symptoms on cranberry fruits, they were classified as the genus Diaporthe. Further analysis of ITS sequence data allowed for the classification of the newly obtained isolates as D. vaccinii. Additional analysis of genetic diversity using five RAPD and eight ISSR primers constituted additional confirmation of genetic distance existing between closely related D. vaccinii and D. eres species, enabling their differentiation.

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“…The genera Diaporthe and Pestalotia/Pestalotiopsis are classified as fungal pathogens globally causing canker and dieback in legumes, multiple fruits and woody plants (Espinoza et al 2008;Diogo et al 2010;Urbez-Torres et al 2013). …”

Section: Fungal Pathogensmentioning

confidence: 99%

Mimosa pigra dieback in the Northern Territory, Australia: Investigation into possible causes

Sacdalan¹

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In this study, Mimosa pigra dieback is described as plants or population of plants which appeared to suffer from ill health, death of their growing points and the emergence of green reshoots coming from the base. Dieback is an important phenomenon, not only in many crops and natural forests worldwide, but also in many invasive trees and shrubs in Australia. The dieback phenomenon is thought to be caused by complex and interacting factors and causes are often difficult to disentangle. In some cases it is a result of an interaction between both biotic and abiotic factors. Since 1988, dieback has been widely observed in Mimosa pigra L.(Fabaceae), a woody shrub that is one of the most serious exotic invaders of floodplains and riparian areas in the Top End of the Northern Territory. However, there has been no conclusive evidence of links between M. pigra dieback and specific causal factors. In addition, the dieback phenomenon has not been previously quantified.This thesis aimed to describe M. pigra dieback phenomenon at plant and population levels and its effect on plant growth, survival and demography. Specifically, the aim of this thesis was to evaluate the role of biotic factors (insect borers and fungal pathogens), and abiotic factors (soil pH and salinity), in relation to the development of M. pigra dieback. In addition, the thesis examines the pathogenicity of the fungal endophytes/pathogens associated with diebackaffected and healthy M. pigra. M. pigra has been a focus of biological control programs for more than 30 years, but it still remains a serious threat in the wetlands and conservation areas in the Top End of the Northern Territory. While extensive studies have been conducted to evaluate the impact of biological control programs, little is actually known about dieback and its causal factors.To evaluate the causal factors of M. pigra dieback, repeated ground surveys, plant health assessment and specimen collection of stems and soils, were conducted in the period 2011 to 2013 in established M. pigra sites on Melaleuca Station, Mary River, Northern Territory. Insect borers were quantified and putative fungal pathogen abundance were surveyed. A combined morphological and phylogenetic approach was used for fungal pathogens/endophyte identification. Using a subset of isolates, trials were performed to examine differences in the pathogenicity of the recovered fungal pathogens/endophytes.Results of field research showed that dieback in M. pigra is highly dynamic and fluctuates in its severity. Different sites showed increases and/or decreases in plant health over time.iii Dieback affects plants in all age classes, from seedlings, juveniles to adult plants, resulting in partial mortality of plants in all age categories over the trial period. Contrary to expectations, none of the biotic and abiotic factors examined showed a direct relationship to the changing population dynamics of M. pigra dieback over time. Nevertheless, fungal isolations demonstrated that M. pigra is a host to a diverse range of endop...

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Phylogeny, morphology and pathogenicity of Diaporthe and Phomopsis species on almond in Portugal

Cited by 94 publications

References 20 publications

Grapevine Trunk Diseases Associated with Fungi from the Diaporthaceae Family in Croatian Vineyards / Identifikacija Vrsta Roda Fusarium Izoliranih S Plodova Jabuke Nakon Skladištenja

Grapevine Trunk Diseases Associated with Fungi from the Diaporthaceae Family in Croatian Vineyards / Identifikacija Vrsta Roda Fusarium Izoliranih S Plodova Jabuke Nakon Skladištenja

Identification and characterization of Diaporthe vaccinii Shear causing upright dieback and viscid rot of cranberry in Poland

Mimosa pigra dieback in the Northern Territory, Australia: Investigation into possible causes

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