Identification and characterization of Diaporthe vaccinii Shear causing upright dieback and viscid rot of cranberry in Poland

Abstract

During the summers of 2013-2014, symptoms similar to viscid rot and upright disease were observed on cranberries (Vaccinium macrocarpon) on one plantation in central Poland. The associated fungi were isolated from symptomatic plant tissue. On the basis of morphological and cultural characteristics and the ability of isolated fungi to elicit viscid rot symptoms on cranberry fruits, they were classified as the genus Diaporthe. Further analysis of ITS sequence data allowed for the classification of the newly obtained isolates as D. vaccinii. Additional analysis of genetic diversity using five RAPD and eight ISSR primers constituted additional confirmation of genetic distance existing between closely related D. vaccinii and D. eres species, enabling their differentiation.

Pathogenicity of Diaporthe spp. on two blueberry cultivars (Vaccinium corymbosum)

“…The level of isolation was similar for D. vaccinii and D. eres. The growth rate of D. eres is higher than that of D. vaccinii on PDA medium, as described previously in the literature (Michalecka et al, 2017). 4.…”

“…Diaporthe vaccinii has been found in several countries in Europe, for example Latvia, Lithuania, Poland and the Netherlands (Lombard et al, 2014;Vilka & Volkova, 2015;Michalecka et al, 2017). Currently, the pathogen is only listed as present in Latvia (EPPO Global Database), and has been eradicated from Poland and the Netherlands (EPPO, 2015;NPPO-NL, 2015).…”

First detection of Diaporthe eucommiae causing leaf black blight disease on Cyclocarya paliurus

“…On the other hand, Diaporthe/Phomopsis species with relatively low identity (97%‐98%) of ITS sequences could not be precluded to be within the same species. Michalecka, Bryk, and Seliga () reported that the ITS sequence of the strain Lat4, which was obtained from diseased cranberry showing upright dieback and viscid rot symptoms, shared 97.4% similarity with the sequences of Diaporthe eres CBS 524.82 from Poland and of ex‐epitype D. eres CBS 138594 from Germany. The strain Lat4 was identified as D. eres due to falling into the same group with the two D. eres mentioned above according to ITS sequences.…”

“…Of late, Michalecka et al. () used ITS sequence data, plus morphological and cultural characteristics to characterize D. vaccinii species associated with upright dieback and viscid rot of cranberry in Poland. The phylogenetic analysis based on ITS sequences clearly differentiated the examined D. vaccinii strains from other Diaporthe / Phomopsis clades.…”

“…On the other hand, Diaporthe/Phomopsis species with relatively low identity (97%-98%) of ITS sequences could not be precluded to be within the same species. Michalecka, Bryk, and Seliga (2017) Despite that the ITS region in Diaporthe/Phomopsis appears to be evolving faster than EF1α or even mating type (MAT) genes, ITS sequence data can be used for reliable identification of phylogenetic relationships if they are interpreted with care (Udayanga et al, 2011). A phylogenetic tree using type-derived ITS sequences can be a rough and quick identification guide for species of Phomopsis (Udayanga et al, 2011).…”