Phylogeny and geographical differentiation of zucchini yellow mosaic virus isolates (Potyviridae) based on molecular analysis of the coat protein and part of the cytoplasmic inclusion protein genes

Abstract

DNA sequences for the coat protein (CP) and part of the cytoplasmic inclusion (CI) protein genes after reverse transcriptase-polymerase chain reaction (RT-PCR) were reported for the first time for isolates of zucchini yellow mosaic virus (ZYMV) from Austria, Germany, Italy and Slovenia. Comparison of the DNA sequences of 30 isolates from different geographical regions worldwide revealed highest similarities of the Austrian isolates to those from Slovenia and Hungary. Isolates from Germany and Italy were only distantly related and clustered with isolates from other parts of the world. Our results indicate that a specific isolate can spread rapidly to geographically adjacent areas but may not be directly related to isolates found in other neighboring countries.

Sequence diversity, population genetics and potential recombination events in grapevine rupestris stem pitting-associated virus in Pacific North-West vineyards

“…In view of such discordant gene phylogenies among GRSPaV isolates, it seemed appropriate to use one of these two genes for comparative sequence analysis and we favour the whole CP gene sequences as the best representation of the phylogeny of global isolates of GRSPaV. Such CP-based phylogeny has been used for the classification of several different virus groups (Karan et al, 1994;Pfosser & Baumann, 2002;Fajardo et al, 2007).…”

Rapid evolutionary dynamics of zucchini yellow mosaic virus

“…However, isolates sampled from adjoining locations are not always related (Pfosser & Baumann, 2002), suggesting that biogeographical structure may, to some extent, be determined by the international trading of infected seeds (Desbiez et al, 2002;Tobias & Palkovics, 2003).…”

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

“…It is most likely that a specific ZYMV isolate spread rapidly to the cucurbit-growing area of the Vojvodina Province, which is a part of the Central European region. This could also be an explanation for the first massive outbreak of ZYMV in Serbia in 2000 (Dukić et al 2002), as it occurred in Austria, Hungary and Slovenia almost at the same time (Pfosser and Baumann 2002). Phylogenetic data in this study indicated that there was no new introduction of independent ZYMV isolates in Serbia.…”

“…The phylogenetic analysis also revealed that Serbian isolates and those from Central Europe shared a recent common ancestor or had been spread from a unique infection focus, which could provide a clue to the origin of ZYMV isolates from Serbia. This study extended the information provided by Pfosser and Baumann (2002) that a single introduction of ZYMV occurred in the geographically adjacent areas of Austria, Slovenia, and Hungary. It is most likely that a specific ZYMV isolate spread rapidly to the cucurbit-growing area of the Vojvodina Province, which is a part of the Central European region.…”