Phylogeny and Evolution of Orchis and Allied Genera Based on ITS DNA Variation: Morphological Gaps and Molecular Continuity

Aceto, Serena; Caputo, Paolo; Cozzolino, Salvatore; Gaudio, Luciano; Moretti, Aldo

doi:10.1006/mpev.1999.0628

Cited by 125 publications

(104 citation statements)

References 47 publications

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“…PCR products generated with ITS primers were sequenced according to Aceto et al (1999a). To identify the origin of pollinaria, ITS sequences were subjected to a FASTA search against GenBank or compared with unpublished orchid sequences datasets (Cozzolino et al 2001;Bateman et al 2003).…”

Section: Methodsmentioning

confidence: 99%

Evidence for pollinator sharing in Mediterranean nectar-mimic orchids: absence of premating barriers?

Cozzolino

Schiestl²,

Müller³

et al. 2005

Proc. R. Soc. B.

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Pollinator specificity has traditionally been considered the main reproductive isolation mechanism in orchids. Among Mediterranean orchids, however, many species attract and deceive pollinators by mimicking nectar-rewarding plants. To test the extent to which deceptive orchid species share pollinators, we collected and identified hemipollinaria-carrying insects, and used ribosomal sequences to identify the orchid species from which hemipollinaria were removed. We found that social and solitary bees, and also flies, carried hemipollinaria belonging to nine orchid species with different degrees of specialization. In particular, Anacamptis morio, Dactylorhiza romana and Orchis mascula used a large set of pollinator species, whereas others such as Orchis quadripunctata seemed to be pollinated by one pollinator species only. Out of the insects with hemipollinaria, 19% were found to carry hemipollinaria from more than one orchid species, indicating that sympatric food-deceptive orchids can share pollinators. This sharing was apparent even among orchid sister-species, thus revealing an effective overlap in pollinator sets among closely related species. These results suggest varying degrees of pollinator specificity in these orchids, and indicate that pollinator specificity cannot always act as the main isolation mechanism in food-deceptive temperate orchids.

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Section: Methodsmentioning

confidence: 99%

Evidence for pollinator sharing in Mediterranean nectar-mimic orchids: absence of premating barriers?

Cozzolino

Schiestl²,

Müller³

et al. 2005

Proc. R. Soc. B.

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“…Based on conclusion that variation in morphology and molecular markers correspond closely (Aceto et al 1999), the taxonomically closer plants and those with more similar morphologies are also expected to be more similar genetically. Of all the plants researched Serapias istriaca is most similar to S. ionica by its habitus and morphology (Perko 1998).…”

Section: Genetic Differencesmentioning

confidence: 99%

Morphometric and molecular (RAPD) analysis of six Serapias taxa from Croatia

et al. 2010

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Abstract:The morphometric analyses and genetic variability assessed by RAPD markers have been used to analyse relations among six Serapias taxa from Croatia (S. istriaca, S. pulae originally described as hybrid, S. ionica, S. vomeracea, S. lingua and S. cordigera). S. istriaca distributed in southern Istria and the island of Lošinj and S. pulae stenoendemic taxon distributed only in southern Istria S. ionica is endemic to the Ionian and Dalmatian islands, while the remaining taxa are more widely distributed. The obtained results shows that the endemic S. istriaca is a well characterised taxon, that S. pulae is a hybrid between S. istriaca and S. lingua and that the hybrid is morphologically and genetically more similar to S. lingua than the second parental species S. istriaca. The division into the subsections Steno-, Medio-and Platypetalae is founded based on the floral morphology while the division into the sections Serapias and Bilamellaria is not evident in the quantitative morphological and genetic analyses. Furthermore, considerable genetic resemblance between S. vomeracea and S. ionica was established.

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“…In the previous studies were reported that the genetic variation of orchid species also shows high variation [26][27][28]. The range of high genetic distance values based on the results of Nei's unbiased distance analysis is reflected from the highly variable phenotype of orchid flowers ( Figure 2).…”

Section: Genetic Variationsmentioning

confidence: 85%

Genetic Variations and Relationships of Papua’s Endemic Orchids Based on RAPD Markers

Abbas¹,

Dailami²,

Listyorini³

et al. 2017

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Orchidaceae has known as an attractive flower and immense species. We have found a large species of Orchidaceae grow naturally in Papua's jungle, Indonesia territorial. This study aims to reveal genetic variation and genetic relationships among endemic orchids in Papua based on RAPD markers. The study included 26 accessions of Papua's endemic orchids used for genomic DNA extraction. Genomic DNAs were extracted by using DNA extraction kit from Qiagen and genomic DNA amplification by using 10 decamer RAPD primers. DNA fragments that were amplified by Polymerase Change Reaction (PCR) were visualized and documented by using UV illumination apparatus. Genetically, endemic Orchids in Papua were described high variation. Fragments amplification by using ten RAPD primers and performed in the PCR tools resulted in 54 numbers of polymorphic fragments and no monomorphic band. The number of polymorphic bands per primer ranged from 4 to 7 with averaged 5.4 bands per assay unit. The genetic dissimilarities (GDs) among examined orchids ranged from 0.10 to 0.94 based on Nei's unbiased coefficients. Dendrogram construction showed that Papua's endemic orchid (PEO) samples different from another and separated to form group by their own at the 0.40 coefficient value and at the 0.6 coefficient value indicate that PEO sample is divided into nine groups i.e. samples at the genera level were separated into their own groups.

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Phylogeny and Evolution of Orchis and Allied Genera Based on ITS DNA Variation: Morphological Gaps and Molecular Continuity

Cited by 125 publications

References 47 publications

Evidence for pollinator sharing in Mediterranean nectar-mimic orchids: absence of premating barriers?

Evidence for pollinator sharing in Mediterranean nectar-mimic orchids: absence of premating barriers?

Morphometric and molecular (RAPD) analysis of six Serapias taxa from Croatia

Genetic Variations and Relationships of Papua’s Endemic Orchids Based on RAPD Markers

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