Phylogeny and evolution of Newcastle disease virus genotypes isolated in Asia during 2008–2011

Ebrahimi, Mohammad Majid; Shahsavandi, Shahla; Moazenijula, Gholamreza; Shamsara, Mehdi

doi:10.1007/s11262-012-0738-5

Cited by 58 publications

(36 citation statements)

References 27 publications

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“…However, it is still enzootic in some areas and is recognized as a major disease that affects poultry [3]. The genotype VII NDV is dominant in Asia and poses a serious threat to its poultry industry [4–6]. Previous studies have revealed that the significant oviduct inflammation and decline of egg production are caused by the variance [2, 7].…”

Section: Introductionmentioning

confidence: 99%

Strong inflammatory responses and apoptosis in the oviducts of egg-laying hens caused by genotype VIId Newcastle disease virus

Guo

Liu

et al. 2016

BMC Vet Res

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BackgroundNewcastle disease virus (NDV) can cause serious damage to the reproductive tracts of egg-laying hens and leads to egg production and quality reduction. However, the mechanism of severe pathological damage in the oviducts of egg-laying hens after NDV infection has not been fully elucidated. In this study, the correlation between the primary pathological lesions and viral load in the oviducts of egg-laying hens infected with the velogenic genotype VIId NDV strain was evaluated by pathological observation and virus detection. Subsequently, apoptosis, the expression of immune-related genes and lymphocyte infiltration into the infected oviducts were determined to explore the potential causes of the pathological changes.ResultsA higher viral load and severe tissue lesions and apoptotic bodies were observed in the oviduct of NDV-infected hens compared with the control. Immune-related genes, including TLR3/7/21, MDA5, IL-2/6/1β, IFN-β, CXCLi1/2, and CCR5, were significantly upregulated in the magnum and uterus. IL-2 presented the highest mRNA level change (137-fold) at 5 days post infection (dpi) in the magnum. Infection led to CD3+CD4+ and CD3+CD8α+ lymphocyte infiltration into the magnum of the oviduct. A higher viral load was found to be associated with pathological changes and the elevated expression of proinflammatory cytokines in the NDV-infected hens.ConclusionsOur results indicate that the severe lesions and apoptosis in the oviducts of egg-laying hens caused by genotype VIId NDV strains are associated with the excessive release of inflammatory cytokines, chemokines and lymphocyte infiltration, which contribute to the dysfunction of the oviducts and the decrease of egg production in hens.

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Section: Introductionmentioning

confidence: 99%

Strong inflammatory responses and apoptosis in the oviducts of egg-laying hens caused by genotype VIId Newcastle disease virus

Guo

Liu

et al. 2016

BMC Vet Res

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“…In the past few decades, implementation of extensive vaccination programs in commercial poultry farms, and to some extent in small rural poultry farms have reduced the number of epizootics outbreaks of ND in Iran. In recent year so many reports for emerging of velogneic ND in commercial Poultry and ostrich farms (8,9). In this study analyze fusion protein gene of recently NDV isolated from ostrich in Iran, 2012.…”

Section: Introductionmentioning

confidence: 99%

Sequence Analysis of Fusion Gene of Newcastle Disease Viruses Isolated from Ostrich (Struthio camelus) in Iran, 2012

et al. 2011

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Background and Aims: Newcastle disease virus (NDV) infection have been established in at least 241species of birds representing 27 of the 50 orders of the class.NDV isolate were obtained from infected ostrich flock during the outbreaks of ND in Iran 2012. The F gene fragment which codes the main functional region of the F protein was obtained by RT-PCR and sequenced. Methods: From the pathotype prediction based on the cleavage site of the fusion protein, this isolate was placed into the velogenic group with the motif 112 RRQKRF 117. Results: Phylogenetic analysis based on a partial F gene sequence showed that the isolates from ostrich cluster together with concurrent isolates from poultry in Iran within the sub genotype VIId, which is the predominant pathogen involved currently in Newcastle disease outbreaks in poultry worldwide. Conclusion:This study adds to the understanding of the ecology of NDV in ostrich and emphasizes the need for constant surveillance in times of an ongoing and expanding epidemic of NDV. This finding is essential for improving the disease control strategies and development of vaccines for ND.

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“…Among them genotype VII, representing viruses that emerged in the 1990s in the Far East were prevalent and mainly responsible for ND outbreaks in Far East, Europe, and South Africa. The genotype was divided into VIIaVIIh subgenotypes in which VIIa-VIIe were circulating predominantly in China, Malaysia, Kazakhstan and Kyrgyzstan mainly responsible for recent outbreaks and VIIf-VIIh represent African isolates [6]. The genotype VI group contains viruses from around the world collected as early as 1978.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Study of Newcastle Disease Viruses Isolated in Iran, 2014–2015

et al. 2016

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Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide and the pathogenicity of Newcastle disease virus (NDV) strains varies with different virulence. Samples were collected from chicken commercial farms in Iran during 2014-2015. ND virus was characterized (NDV) by partial sequences of fusion genome and compared with other NDV sequences. All viruses showed the amino acid sequence 112 RRQKRF117 at the C-terminus of the F2 protein and phenylalanine at the N-terminus of the F1 protein, residue 117. These amino acid sequences were identical to a known virulent motif. Based on the phylogenetic analysis the Iranian NDV isolates are closely related to the genotype VIId of class II NDV strains. The result showed that the genotype VIId was dominant and circulating among chicken farms and caused clinical disease.

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Phylogeny and evolution of Newcastle disease virus genotypes isolated in Asia during 2008–2011

Cited by 58 publications

References 27 publications

Strong inflammatory responses and apoptosis in the oviducts of egg-laying hens caused by genotype VIId Newcastle disease virus

Strong inflammatory responses and apoptosis in the oviducts of egg-laying hens caused by genotype VIId Newcastle disease virus

Sequence Analysis of Fusion Gene of Newcastle Disease Viruses Isolated from Ostrich (Struthio camelus) in Iran, 2012

Molecular Characterization and Phylogenetic Study of Newcastle Disease Viruses Isolated in Iran, 2014–2015

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