Sequence Analysis of Fusion Gene of Newcastle Disease Viruses Isolated from Ostrich (Struthio camelus) in Iran, 2012

Ghalyanchilangeroudi, Arash; Hosseini, Hossein; Madadgar, Omid; Karimi, Vahid; Shahraeini, A; Ghafari, M M

doi:10.21859/isv.5.3.12

Cited by 6 publications

(3 citation statements)

References 12 publications

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“…Alternatively, natural recombination could have occurred at a certain point in time between genotype VII-like and genotype II-like viruses. Similar situations were previously reported in China and Iran [20,35,38-40]. A complete genome sequence of a similar NDV isolate revealed that recombination had occurred at the N terminus of the F gene [39].…”

Section: Resultssupporting

confidence: 78%

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Molecular and biological characterization of some circulating strains of Newcastle disease virus in broiler chickens from Eastern Saudi Arabia in 2012-2014

Al-Mubarak

2019

Vet World

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Background and Aim: Newcastle disease (ND) is a worldwide poultry disease that is historically known to cause severe losses in the poultry industry. In the present study, attempts were made to characterize ND virus (NDV) recovered from broiler chickens in the Eastern Region of Saudi Arabia from January 2012 to March 2014. Materials and Methods: Reverse transcription-polymerase chain reaction was used for the detection of NDV followed by partial sequencing of the fusion (F) gene. The intracerebral pathogenicity index (ICPI), mean death time (MDT), and complete sequencing of the hemagglutinin-neuraminidase (HN) gene were also used for further biological and molecular characterization. Results: NDV was detected at a rate of 9.6% (11/115) of the tested flocks, most of which were vaccinated against ND. F gene-based phylogeny and motifs of the fusion protein cleavage site (FPCS) showed segregation of Saudi isolates into two groups. The first group contained 10 isolates and was located in genotype II with the lentogenic motif 112GRQGRL117 at the FPCS. The second group contained one isolate and was located in genotype VII with velogenic motif 112RRQKRF117. Further characterization using the ICPI and MDT of two representative isolates showed virulence of both tested isolates. Phylogenetic analysis of the HN gene showed close nucleotide identity between the two isolates. A BLAST search for sequences similar to HN gene sequences showed high identity with isolates from the surrounding region. Conclusion: The present findings showed a low detection rate of NDV, possibly due to the wide application of vaccines, and the circulation of at least two NDV genotypes, II and VII, in the Eastern Region of Saudi Arabia. The present Saudi isolates may share common ancestors with isolates from the surrounding region.

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Section: Resultssupporting

confidence: 78%

“…**Sequences NDV-SA/Chicken/NC19 and NDV-SA/Chicken/NC37 showed complete identities with the NDV-SA/Chicken/NH3 sequence. NDV=Newcastle disease virus [20,35,[38][39][40]. A complete genome sequence of a similar NDV isolate revealed that recombination had occurred at the N terminus of the F gene [39].…”

Section: Complete Hn Gene-based Phylogenymentioning

confidence: 99%

Molecular and biological characterization of some circulating strains of Newcastle disease virus in broiler chickens from Eastern Saudi Arabia in 2012-2014

Al-Mubarak

2019

Vet World

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“…ICPI is the generally accepted method to measure the virulence of AAvV-1strains because of its high accuracy and sensitivity [3]. Few authors even reported incongruencies between FPCS and invivo pathogenicity test [16,17].…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Detection of Velogenic Genotype XIII 2.2 Avian Avulavirus Type 1 (AAvV-1) From Spot-billed Pelican and Backyard Chicken: Implications to the Viral Maintenance and Spread

Balam¹,

Ratnamma²,

Isloor³

et al. 2021

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The present study demonstrates simultaneous isolation of genetically similar velogenic Avian Avula virus-1 from an apparently healthy spot- billed pelican and a naturally infected backyard chicken in the adjacent vicinity. A total of fourty eight cloacal swab samples from migratory birds (Painted storks, n=32 and Spot billed pelicans, n=16) at the Telineelapuram bird sanctuary in Andhra Pradesh, India and tissue samples of dead backyard chicken were collected. Two isolates were recovered, one each from a spot billed pelican (MIG-9) and a dead chicken (SKLM-1). The isolates were confirmed as velogenic based on mean death time, intra cerebral pathogenicity index and the putative fusion protein cleavage site (113R-R-K-R-F117). Phylogenetic analysis based on full- length fusion and attachment (HN) proteins classified the isolates into genotype XIII, sub- genotype 2.2. Sequence analysis revealed that the pelican and chicken isolates were 100% identical. The study isolates demonstrated multiple amino acid substitutions at several critical domains of F and HN proteins when compared with the current vaccine strains. Pathogenic and transmission potential of the AAvV-1 isolates was evaluated in three- week old chicken and the isolates proved to be highly virulent to chicken. To the best of our knowledge, this is the first evidence for the role of spot-billed pelicans in the maintenance of virulent AAvV-1 and its transmission to chicken. This study further highlights the role of wild birds in AAvV-1 transmission and the need for enhanced biosecurity in commercial poultry operations.

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Molecular characterization and phylogenetic study of the hemagglutinin-neuraminidase gene of newcastle disease virus isolated from peacock (Pavo cristatus) and Turkey (Meleagris) and its comparison with broiler isolates

et al. 2023

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Sequence Analysis of Fusion Gene of Newcastle Disease Viruses Isolated from Ostrich (Struthio camelus) in Iran, 2012

Cited by 6 publications

References 12 publications

Molecular and biological characterization of some circulating strains of Newcastle disease virus in broiler chickens from Eastern Saudi Arabia in 2012-2014

Molecular and biological characterization of some circulating strains of Newcastle disease virus in broiler chickens from Eastern Saudi Arabia in 2012-2014

Simultaneous Detection of Velogenic Genotype XIII 2.2 Avian Avulavirus Type 1 (AAvV-1) From Spot-billed Pelican and Backyard Chicken: Implications to the Viral Maintenance and Spread

Molecular characterization and phylogenetic study of the hemagglutinin-neuraminidase gene of newcastle disease virus isolated from peacock (Pavo cristatus) and Turkey (Meleagris) and its comparison with broiler isolates

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