Phylogenetic Taxonomy of <i>Artemisia</i> L. Species from Kazakhstan Based on <i>Mat</i>k Analyses

Turuspekov, Yerlan; Genievskaya, Yuliya; Baibulatova, Aida; Затыбеков, Алибек; Kotuhov, Yuri; Ishmuratova, Margarita; Imanbayeva, Akzhunis; Абугалиева, Сауле

doi:10.1515/prolas-2017-0068

Cited by 10 publications

(12 citation statements)

References 26 publications

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“…This work is part of a national program aiming to evaluate the genetic variation in the wild flora of Kazakhstan [28]. The project, along with DNA barcoding analyses of wild plant species [29][30][31][32], is also incorporating studies using simple sequence repeats (SSR) [33], amplified fragment length polymorphism (AFLP) [34], and single nucleotide polymorphism (SNP) [35] markers in a collaboration between local botanists and geneticists.…”

Section: Introductionmentioning

confidence: 99%

The molecular taxonomy of three endemic Central Asian species of Ranunculus(Ranunculaceae)

et al. 2020

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Worldwide, the genus Ranunculus includes approximately 600 species and is highly genetically diverse. Recent taxonomic reports suggest that the genus has a monophyletic origin, divided into two subgenera, and consists of 17 sections. The Central Asian country of Kazakhstan has 62 species of the genus that have primarily been collected in the central part of the country. The latest collection trips in southern parts of the country have led to the description of a wider distribution area for Ranunculus and the identification of a new species Ranunculus talassicus Schegol. et A.L. Ebel from Western Tien Shan. Therefore, in this study, attempts were made to assess the molecular taxonomic positions of R. talassicus and two other species endemic to the Central Asian region R. karkaralensis Schegol. and R. pskemensis V.N. Pavlov in relation to other species of the genus, using internal transcribed spacer (ITS) molecular genetic markers. The ITS-aligned sequences of 22 local Central Asian accessions and 43 accession sequences available in the National Center for Biotechnology Information (NCBI) database allowed the construction of a maximum parsimony phylogenetic tree and a Neighbor-Net network. The results indicated that R. talassicus and R. pskemensis could be assigned to section Ranunculastrum. Additionally, an assessment of the network suggested that R. pskemensis was the rooting taxon for the group of species containing R. talassicus, and that R. illyricus L. and R. pedatus Waldst. & Kit. were founders of a prime rooting node for the Ranunculastrum section of the genus. The ITS-aligned sequences showed that R. karkaralensis was indifferent with respect to three other species in the Ranunculus section of the genus, i.e., R. acris L., R. grandifolius C.A. Mey., and R. subborealis Tzvelev. The study indicated that the assessments of ITS-based phylogenetic tree and Neighbor-Net network provided new insights into the taxonomic positions of three endemic species from Central Asia.

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Section: Introductionmentioning

confidence: 99%

The molecular taxonomy of three endemic Central Asian species of Ranunculus(Ranunculaceae)

et al. 2020

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“…Traditionally, the taxonomic identification of Artemisia species has relied on morphological characteristics. However, controversies with regards to the taxonomic relationships have arisen due to the plastic nature, complex genetics, and polyploidy of Artemisia species, limiting the role of morphological characteristics as the sole factor for classification [29,30]. The development of high-resolution genetic markers is important for fostering attempts regarding species identification, inferring phylogeny, and improving the medicinal value of A. scoparia .…”

Section: Introductionmentioning

confidence: 99%

Chloroplast Genome Sequence of Artemisia scoparia: Comparative Analyses and Screening of Mutational Hotspots

Iram

Hayat

Tahir

et al. 2019

Plants

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Artemisia L. is among the most diverse and medicinally important genera of the plant family Asteraceae. Discrepancies arise in the taxonomic classification of Artemisia due to the occurrence of multiple polyploidy events in separate lineages and its complex morphology. The discrepancies could be resolved by increasing the genomic resources. A. scoparia is one of the most medicinally important species in Artemisia. In this paper, we report the complete chloroplast genome sequence of Artemisia scoparia. The genome was 151,060 bp (base pairs), comprising a large single copy (82,834 bp) and small single copy (18,282 bp), separated by a pair of long inverted repeats (IRa and IRb: 24,972 bp each). We identified 114 unique genes, including four ribosomal RNAs, 30 transfer RNAs, and 80 protein-coding genes. We analysed the chloroplast genome features, including oligonucleotide repeats, microsatellites, amino acid frequencies, RNA editing sites, and codon usage. Transversion substitutions were twice as frequent as transition substitutions. Mutational hotspot loci included ccsA-ndhD, trnH-psbA, ndhG-ndhI, rps18-rpl20, and rps15-ycf1. These loci can be used to develop cost-effective and robust molecular markers for resolving the taxonomic discrepancies. The reconstructed phylogenetic tree supported previous findings of Artemisia as a monophyletic genus, sister to the genus Chrysanthemum, whereby A. scoparia appeared as sister to A. capillaris.

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“…In the past, because of the number of species, diverse morphological types, ploidy, and complicated genetic relationships of Artemisia , the taxonomic relationships of the genus are controversial and based only on morphological traits, such as the capitula type and floret fertility[19, 20]. As a result, considering the conserved structural and relatively compact gene density, chloroplast genomic materials are widely used in genomic evolution studies, molecular marker development, and phylogenetic analysis of the genus Artemisia .…”

Section: Introductionmentioning

confidence: 99%

“…As a result, considering the conserved structural and relatively compact gene density, chloroplast genomic materials are widely used in genomic evolution studies, molecular marker development, and phylogenetic analysis of the genus Artemisia . Many researchers have used single gene data ( matK , ndhF , rps11 ), IGS data ( psbA _ trnH , trnS _ trnC , trnS _ trnfM , trnL _ trnF ), and shared protein-coding gene data of Artemisia to perform phylogenetic analysis [19–27]. However, the cp genomic data of Artemisia are still quite limited and data for only a few species have been reported.…”

Section: Introductionmentioning

confidence: 99%

Detecting useful genetic markers and reconstructing the phylogeny of an important medicinal resource plant, Artemisia selengensis, based on chloroplast genomics

et al. 2019

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Artemisia selengenesis is not only a health food, but also a well-known traditional Chinese medicine. Only a fraction of the chloroplast (cp) genome data of Artemisia has been reported and chloroplast genomic materials have been widely used in genomic evolution studies, molecular marker development, and phylogenetic analysis of the genus Artemisia, which makes evolutionary studies, genetic improvement, and phylogenetic identification very difficult. In this study, the complete chloroplast genome of A. selengensis was compared with that of other species within Artemisia and phylogenetic analyses was conducted with other genera in the Asteraceae family. The results showed that A. selengensis is an AT-rich species and has a typical quadripartite structure that is 151,215 bp in length. Comparative genome analyses demonstrated that the available chloroplast genomes of species of Artemisia were well conserved in terms of genomic length, GC contents, and gene organization and order. However, some differences, which may indicate evolutionary events, were found, such as a re-inversion event within the Artemisia genus, an unequal duplicate phenomenon of the ycf1 gene because of the expansion and contraction of the IR region, and the fast-evolving regions. Repeated sequences analysis showed that Artemisia chloroplast genomes presented a highly similar pattern of SSR or LDR distribution. A total of 257 SSRs and 42 LDRs were identified in the A. selengensis chloroplast genome. The phylogenetic analysis showed that A. selengensis was sister to A. gmelinii. The findings of this study will be valuable in further studies to understand the genetic diversity and evolutionary history of Asteraceae.

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Phylogenetic Taxonomy of Artemisia L. Species from Kazakhstan Based on Matk Analyses

Abstract: The genus

Cited by 10 publications

References 26 publications

The molecular taxonomy of three endemic Central Asian species of Ranunculus(Ranunculaceae)

The molecular taxonomy of three endemic Central Asian species of Ranunculus(Ranunculaceae)

Chloroplast Genome Sequence of Artemisia scoparia: Comparative Analyses and Screening of Mutational Hotspots

Detecting useful genetic markers and reconstructing the phylogeny of an important medicinal resource plant, Artemisia selengensis, based on chloroplast genomics

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