The molecular taxonomy of three endemic Central Asian species of Ranunculus(Ranunculaceae)

Abstract

Worldwide, the genus Ranunculus includes approximately 600 species and is highly genetically diverse. Recent taxonomic reports suggest that the genus has a monophyletic origin, divided into two subgenera, and consists of 17 sections. The Central Asian country of Kazakhstan has 62 species of the genus that have primarily been collected in the central part of the country. The latest collection trips in southern parts of the country have led to the description of a wider distribution area for Ranunculus and the identification of a new species Ranunculus talassicus Schegol. et A.L. Ebel from Western Tien Shan. Therefore, in this study, attempts were made to assess the molecular taxonomic positions of R. talassicus and two other species endemic to the Central Asian region R. karkaralensis Schegol. and R. pskemensis V.N. Pavlov in relation to other species of the genus, using internal transcribed spacer (ITS) molecular genetic markers. The ITS-aligned sequences of 22 local Central Asian accessions and 43 accession sequences available in the National Center for Biotechnology Information (NCBI) database allowed the construction of a maximum parsimony phylogenetic tree and a Neighbor-Net network. The results indicated that R. talassicus and R. pskemensis could be assigned to section Ranunculastrum. Additionally, an assessment of the network suggested that R. pskemensis was the rooting taxon for the group of species containing R. talassicus, and that R. illyricus L. and R. pedatus Waldst. & Kit. were founders of a prime rooting node for the Ranunculastrum section of the genus. The ITS-aligned sequences showed that R. karkaralensis was indifferent with respect to three other species in the Ranunculus section of the genus, i.e., R. acris L., R. grandifolius C.A. Mey., and R. subborealis Tzvelev. The study indicated that the assessments of ITS-based phylogenetic tree and Neighbor-Net network provided new insights into the taxonomic positions of three endemic species from Central Asia.

A new species of Ranunculus (Ranunculaceae) from Western Pamir-Alay, Uzbekistan

“…The results of the phylogenetic analysis were similar to the results of Hörandl and Emadzade (2012) and Almerekova et al (2020) . Species of R. sect.…”

“…We also used available sequences of 15 Ranunculus species from GenBank ( www.ncbi.nlm.nih.gov/Genbank ) (Table 2 ). We used Ranunculus subgenus Auricomus as the outgroup ( Hörandl and Emadzade 2012 ; Almerekova et al 2020 ).…”

Ranunculus aydogdui(Ranunculaceae), a new species from Turkey

“…Recent phylogenetic and taxonomic data suggest that the genus is monophyletic. It has been divided into two subgenera and 17 sections (Almerekova et al 2020). The general success of Ranunculus in respect to species diversity and distribution might be mainly due to a combination of high morphological plasticity, including a genetic flexibility for a rapid adaptation to new habitats, thus permitting development of various eco‐ and phenotypes, hybridization and polyploidy as factors for diversification, and a broad range of reproductive systems including vegetative growth, autogamy, apomixis and combinations thereof, enabling species to colonize diverse habitats, especially in regions with colder climates (Hörandl et al 2009).…”

“…The classification of the genus is very complex and has not been fully completed. Initial worldwide classifications of Ranunculus were based on the description of achenes, flowers, roots and fruit anatomy (Almerekova et al 2020).…”

Therapeutic Potential of Ranunculus Species (Ranunculaceae): A Literature Review on Traditional Medicinal Herbs

“…Subsequently, DNA markers were utilized to delineate the phylogenetic relationships within Ranunculaceae [14][15][16][17][18][19][20][21][22][23]. The sequences of the internal transcribed spacer region of nuclear ribosomal DNA are mostly used as DNA barcode markers for phylogenetic studies at the generic/subgeneric level [24,25].…”