Phylogenetic relationships, recombination analysis, and genetic variability among diverse variants of tomato yellow leaf curl virus in Iran and the Arabian Peninsula: further support for a TYLCV center of diversity

Hosseinzadeh, Mahnaz; Shams-Bakhsh, Masoud; Osaloo, Shahrokh Kazempour; Brown, Judith K.

doi:10.1007/s00705-013-1851-z

Cited by 19 publications

(22 citation statements)

References 58 publications

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“…Meanwhile, by summarizing to date the breeding efforts in breeding tomato with TYLCV resistance, we demonstrated that a very small number of resources has been used as resistance genitors in commercial cultivars. TYLCV has a great potential to change due to factors including virus recombination, mutations, additions of satellite and invasion of exogenous whitefly species ( Nawaz-ul-Rehman and Fauquet, 2009 ; Czosnek and Ghanim, 2011 ; Hosseinzadeh et al, 2014 ). For example the TYLCV-IS76 strain, a recombinant between TYLCV-IL and the Spanish strain of TYLC-Sardinia virus (TYLCSV-ES), which can accumulate better than its parental strains in cultivars carrying the Ty-1 gene ( Belabess et al, 2015 , 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm

et al. 2018

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Tomato yellow leaf curl virus (TYLCV) is a virus species causing epidemics in tomato (Solanum lycopersicum) worldwide. Many efforts have been focused on identification of resistance sources by screening wild tomato species. In many cases, the accession numbers were either not provided in publications or not provided in a consistent manner, which led to redundant screenings. In the current study, we summarized efforts on the screenings of wild tomato species for TYLCV resistance from various publications. In addition, we screened 708 accessions from 13 wild tomato species using different inoculation assays (i.e., whitefly natural infection and Agrobacterium-mediated inoculation) from which 138 accessions exhibited no tomato yellow leaf curl disease (TYLCD) symptoms. These symptomless accessions include 14 accessions from S. arcanum, 43 from S. chilense, 1 from S. chmielewskii, 28 from S. corneliomulleri, 5 from S. habrochaites, 4 from S. huaylasense, 2 from S. neorickii, 1 from S. pennellii, 39 from S. peruvianum, and 1 from S. pimpinellifolium. Most of the screened S. chilense accessions remained symptomless. Many symptomless accessions were also identified in S. arcanum, S. corneliomulleri, and S. peruvianum. A large number of S. pimpinellifolium accessions were screened. However, almost all of the tested accessions showed TYLCD symptoms. Further, we studied allelic variation of the Ty-1/Ty-3 gene in few S. chilense accessions by applying virus-induced gene silencing and allele mining, leading to identification of a number of allele-specific polymorphisms. Taken together, we present a comprehensive overview on TYLCV resistance and susceptibility in wild tomato germplasm, and demonstrate how to study allelic variants of the cloned Ty-genes in TYLCV-resistant accessions.

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Section: Discussionmentioning

confidence: 99%

Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm

et al. 2018

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“…Total genomic DNA was isolated from 100 mg leaf tissue of infected as well as healthy Amaranthus plant using a DNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany); and begomovirus infection was confirmed by polymerase chain reaction (PCR) using specific primers TYC1F (5'-GGGCCTAGAGACCTG GCCCAC-3') and TYC1R (5'-CCGGTAATATTATAC GGATGGC-3'), which amplify an 856-bp fragment of the 5' end of the C1 gene of TYLCV-IL (Hosseinzadeh et al, 2014). No amplicon was observed in healthy samples.…”

Section: Virus Detection Cloning and Sequencingmentioning

confidence: 99%

“…The sequence identity and phylogenetic analysis results strongly support the causative agent is a variant of Tomato leaf curl Sudan virus (ToLCSDV), a virus that has been reported earlier to cause tomato leaf curl disease in Sudan, Oman and Yemen. The association of begomovirus with leaf curl disease on various crops in Arabian Peninsula has been published in many reports (Ajlan et al, 2007;Khan et al, 2008;Idris et al, 2012;Akhtar et al, 2014;Idris et al, 2011;Khan et al, 2013a;(Ajlan et al, 2007;Khan et al, 2008;Idris et al, 2012;Akhtar et al, 2014;Idris et al, 2011;Khan et al, 2013bAl-Saleh et al, 2014Hosseinzadeh et al, 2014;Idris et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Tomato Leaf Curl Sudan Virus (TLCSDV) causing leaf curl disease on a new host Amaranthus cruentus L.

Sohrab¹

2017

Plant Omics

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Amaranthus leaf curl disease symptom was observed in the farmer's field based at Jeddah, Saudi Arabia. This crop used as leafy vegetables in many countries. In this study, a field survey was conducted in April 2014 and naturally infected Amaranthus leaf samples were collected to identify the associated virus with leaf curl disease. The causative agent was transmitted through whiteflies (Bemisia tabaci) from naturally infected leaves to healthy Amaranthus seedlings. The begomovirus infection was identified by PCR by using specific primers. The full viral genome was amplified by rolling circle amplification. The presence of betasatellites was also confirmed by using betasatellites specific primers. The full viral genomes as well as betasatellites were amplified, cloned and sequenced. The full-length viral genome sequence analysis showed the highest (99.9%) homology with Tomato leaf curl Sudan Virus infecting tomato reported from the Arabian Peninsula. The betasatellites sequence analysis showed the highest identity (99.3%) with Tomato leaf curl betasatellites-Yemen. The phylogenetic analysis was performed by using both full as well as betasatellites genome and full genome formed the closest cluster with Tomato leaf curl Sudan virus while betasatellites genome formed closed cluster with tomato yellow leaf curl Yemen betasatellites. The recombination analysis was performed and results showed that the associated virus could be a variant of Tomato leaf curl Sudan virus, a virus that occurs in Sudan, Yemen and Arabian Peninsula. This is the first report that about the Tomato leaf curl Sudan virus causing leaf curl disease on a new host Amaranthus in Jeddah, Saudi Arabia.

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“…Naturally infected leaf samples from symptomatic cucumber plants displaying characteristic yellow mosaic symptoms (Fig 1) were collected during field survey in April 2014 from farmers field Jeddah, Saudi Arabia. Total genomic DNA was isolated from 100 mg leaf tissue using a DNAeasy plant mini kit (Qiagen Inc.) and begomovirus infection was also confirmed by PCR using specific primers TYC1F (5'-GGGCCTAGAGACCTGGCCCAC-3') and TYC1R (5'-CCGGTAATATTATACGGATGGC-3') (Hosseinzadeh et al, 2014). The presence of betasatellites were also confirmed by amplifying the full-length betasatellites genome by using specific primers; beta01/beta02 (Briddon et al, 2002).…”

Section: Sample Collection Virus Detection and Whiteflies Transmissionmentioning

confidence: 99%

Begomovirus infection on Cucumber in Saudi Arabia

Sohrab¹,

Yasir²,

El-Kafrawy³

2017

Plant Omics

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Cucurbits are an important vegetable crops and among them Cucumber (Cucumis sativus) used mainly as vegetables and salad. The cucumber crop was found to exhibit yellow mosaic symptoms grown in the natural field in Saudi Arabia. We collected naturally infected samples and detected the begomovirus infection by polymerase chain reaction and full length as well as betasatellites viral genome was cloned and sequenced. The sequences of the full length viral genome had 2784 and betasatellites had 1377 nucleotides respectively. In a multiple sequences analysis, highest homology was observed with Tomato yellow leaf curl virus previously reported from Jizan and Al-Qasim, Saudi Arabia. The betasatellites sequences were also analyzed but, interestingly the highest homology was observed with Tomato yellow leaf curl betasatellites reported from Jeddah and Oman. In a phylogenetic tree analysis, the closest cluster was formed with begomovirus isolates identified earlier from Jizan and Al-Qasim. Based on the results obtained in this study, it is concluded that a variant of Tomato yellow leaf curl virus associated with yellow mosaic disease of cucumber in Saudi Arabia. These findings provide valuable information about the natural infection and disease spread caused by begomovirus in new geographic regions on new host.

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Phylogenetic relationships, recombination analysis, and genetic variability among diverse variants of tomato yellow leaf curl virus in Iran and the Arabian Peninsula: further support for a TYLCV center of diversity

Cited by 19 publications

References 58 publications

Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm

Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm

Tomato Leaf Curl Sudan Virus (TLCSDV) causing leaf curl disease on a new host Amaranthus cruentus L.

Begomovirus infection on Cucumber in Saudi Arabia

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