Phylogenetic Relationships of the Litsea Complex and Core Laureae (Lauraceae) Using ITS and ETS Sequences and Morphology<sup>1</sup>

Li, Jie; Conran, John G.; Christophel, David C.; Li, Zhiming; Li, Lang; Li, Hsi-Wen

doi:10.3417/2006125.9504

Cited by 23 publications

(49 citation statements)

References 37 publications

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“…Within the Cryptocarya group, which is basal within Lauraceae [7, 47], Cryptocarya is sister to the non-cupulate clade of Beilschmiedia . Cryptocarya has a deeply urceolate floral hypanthium that develops into a deep cupule enclosing the drupe at maturity, except for a small terminal orifice [7, 46], but Beilschmiedia lacks these characters; a synapomorphy that separates Beilschmiedia and related genera ( Endiandra and Syndiclis ) from the rest of the Cryptocarya group.…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China

Liu

et al. 2017

PLoS ONE

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Lauraceae are an important component of tropical and subtropical forests and have major ecological and economic significance. Owing to lack of clear-cut morphological differences between genera and species, this family is an ideal case for testing the efficacy of DNA barcoding in the identification and discrimination of species and genera. In this study, we evaluated five widely recommended plant DNA barcode loci matK, rbcL, trnH–psbA, ITS2 and the entire ITS region for 409 individuals representing 133 species, 12 genera from China. We tested the ability of DNA barcoding to distinguish species and as an alternative tool for correcting species misidentification. We also used the rbcL+matK+trnH–psbA+ITS loci to investigate the phylogenetic relationships of the species examined. Among the gene regions and their combinations, ITS was the most efficient for identifying species (57.5%) and genera (70%). DNA barcoding also had a positive role for correcting species misidentification (10.8%). Furthermore, based on the results of the phylogenetic analyses, Chinese Lauraceae species formed three supported monophyletic clades, with the Cryptocarya group strongly supported (PP = 1.00, BS = 100%) and the clade including the Persea group, Laureae and Cinnamomum also receiving strong support (PP = 1.00, BS = 98%), whereas the Caryodaphnopsis–Neocinnamomum received only moderate support (PP = 1.00 and BS = 85%). This study indicates that molecular barcoding can assist in screening difficult to identify families like Lauraceae, detecting errors of species identification, as well as helping to reconstruct phylogenetic relationships. DNA barcoding can thus help with large-scale biodiversity inventories and rare species conservation by improving accuracy, as well as reducing time and costs associated with species identification.

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Section: Discussionmentioning

confidence: 99%

DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China

Liu

et al. 2017

PLoS ONE

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“…The bootstrap analysis was performed 1000 times to test the consistency of the nodes of lineage branches in the tree. Neolitsea dealbata was selected as an outgroup because of its close relationship with Cinnamomum (Li et al, 2008).…”

Section: Sequence Analysismentioning

confidence: 99%

A comparison of nrDNA internal transcribed spacer (ITS) loci for phylogenetic inference and authentication among Cinnamomum osmophloeum and related species in Taiwan

Ahmed

Sabry²,

Noh³

et al. 2015

Afr. J. Biotechnol.

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Cinnamomum osmophloeum, an indigenous species of Taiwan, can be utilized for valuable products such as a food, a spice and a traditional Chinese medicine. This study compares the ribosomal DNA (nr DNA) internal transcribed spacer (ITS) sequence of C. osmophloeum to that of several other species with similar external morphology, such as Cinnamomum burmannii, Cinnamomum insularimontanum, Cinnamomum macrostemon and Cinnamomum subavenium. Phylogeny of ITS sequences shows that C. osmophloeum is more closely related to C. burmannii than the other species, while C. insularimontanum, C. macrostemon, and C. subavenium are phylogenetically relevant to each other. By comparing ITS sequence between C. osmophloeum and C. burmannii, specific primers were designed for the multiplex-PCR to differentiate them. Based on ITS sequence differences, all tested Cinnamomum spp. can be properly authenticated. A 125 bp band specific for C. osmophloeum and a 204-bp C. burmannii-specific band were successfully amplified by polymerase chain reaction (PCR) using the respective primers described above. The two species then can be identified at the molecular level according to the sizes of their respective PCR products as determined by gel electrophoresis.

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“…The genera Litsea , Neolitsea , Lindera and Actinodaphne with the smaller genera Dodocadenia , Iteadaphne , Laurus , Parasassafras and Sinosassafras were called the Litsea complex by Li et al (), and their relationships were studied using nuclear DNA ITS and ETS sequences and morphology. Their results showed that the genera Actinodaphne , Lindera and Litsea , as currently circumscribed, are polyphyletic, and that the character of two‐celled vs four‐celled anthers resulted in polyphyletic or paraphyletic genera.…”

Section: Platementioning

confidence: 99%

767. Litsea Populifolia

Rix¹

2013

Curtis's Botanical Magazine

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Phylogenetic Relationships of the Litsea Complex and Core Laureae (Lauraceae) Using ITS and ETS Sequences and Morphology¹

Cited by 23 publications

References 37 publications

DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China

DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China

A comparison of nrDNA internal transcribed spacer (ITS) loci for phylogenetic inference and authentication among Cinnamomum osmophloeum and related species in Taiwan

767. Litsea Populifolia

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Phylogenetic Relationships of the Litsea Complex and Core Laureae (Lauraceae) Using ITS and ETS Sequences and Morphology1

Cited by 23 publications

References 37 publications

DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China

DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China

A comparison of nrDNA internal transcribed spacer (ITS) loci for phylogenetic inference and authentication among Cinnamomum osmophloeum and related species in Taiwan

767. Litsea Populifolia

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Phylogenetic Relationships of the Litsea Complex and Core Laureae (Lauraceae) Using ITS and ETS Sequences and Morphology¹