Phylogenetic relationships of the genus Kluyvera: transfer of Enterobacter intermedius Izard et al. 1980 to the genus Kluyvera as Kluyvera intermedia comb. nov. and reclassification of Kluyvera cochleae as a later synonym of K. intermedia

Paván, María Elisa; Franco, Raúl; Rodríguez, Juan Manuel; Gadaleta, Patricia; Abbott, Sharon L.; Janda, J. Michael; Zorzopulos, Jorge

doi:10.1099/ijs.0.63071-0

Cited by 48 publications

(16 citation statements)

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“…Kluyvera intermedia has been identified in both the intestinal content and the mucosal layer of the rainbow trout O. mykiss (Walbaum) from a commercial farm in Scotland (Kim et al, 2007). It is worth noticing that the authors referred to this bacterium as Enterobacter intermedius; however, this species was recently reclassified in the genus Kluyvera (Pavan et al, 2005). It has also been reported that lactic acid bacteria (LAB) are present in fish intestines (Cai et al, 1998;Ringø & Gatesoupe, 1998;Gonzalez et al, 2000;Ringø et al, 2000), in particular L. lactis, which was isolated as the dominant LAB from the intestine of silver carp, common carp, channel catfish, and deep-bodied crucian carpin in the summer (Hagi et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of intestinal microbiota of rainbow trout (Oncorhynchus mykiss)

Navarrete

Magne

Mardones

et al. 2010

FEMS Microbiology Ecology

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The aim of this study was to evaluate different molecular tools based on the 16S rRNA gene, internal transcribed spacer, and the rpoB gene to examine the bacterial populations present in juvenile rainbow trout intestines. DNA was extracted from both pooled intestinal samples and bacterial strains. Genes were PCR-amplified and analysed using both temporal temperature gradient gel electrophoresis (TTGE) and restriction fragment length polymorphism methods. Because of the high cultivability of the samples, representative bacterial strains were retrieved and we compared the profiles obtained from isolated bacteria with the profile of total bacteria from intestinal contents. Direct analysis based on rpoB-TTGE revealed a simple bacterial composition with two to four bands per sample, while the 16S rRNA gene-TTGE showed multiple bands and comigration for a few species. Sequencing of the 16S rRNA gene- and rpoB-TTGE bands revealed that the intestinal microbiota was dominated by Lactococcus lactis, Citrobacter gillenii, Kluyvera intermedia, Obesumbacterium proteus, and Shewanella marinus. In contrast to 16S rRNA gene-TTGE, rpoB-TTGE profiles derived from bacterial strains produced one band per species. Because the single-copy state of rpoB leads to a single band in TTGE, the rpoB gene is a promising molecular marker for investigating the bacterial community of the rainbow trout intestinal microbiota.

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Section: Discussionmentioning

confidence: 99%

Molecular analysis of intestinal microbiota of rainbow trout (Oncorhynchus mykiss)

Navarrete

Magne

Mardones

et al. 2010

FEMS Microbiology Ecology

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“…MB101::gfp cells by either conjugation or transformation. A more accurate taxonomy of MB101 than previously published was achieved due to a recent reclassification within the Enterobacteriaceae family (Pavan et al, 2005).…”

Section: Construction Of Plasmid Pkjk5 Derivativesmentioning

confidence: 98%

Impact of conjugal transfer on the stability of IncP-1 plasmid pKJK5 in bacterial populations

Bahl

Hansen

Sørensen³

2007

FEMS Microbiology Letters

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The intrinsic stability of IncP-1 plasmid pKJK5 was assessed in both an Escherichia coli and a Kluyvera sp. population maintained in bacterial mats and in liquid nutrient broth without selective pressure. A fluorescence tagging/flow cytometry approach was used to detect and quantify plasmid loss from populations harboring either conjugation-proficient or -deficient pKJK5 derivatives. The results show that the plasmid's ability to conjugate plays an important role in its stable maintenance in populations of both species. This effect was most pronounced in dense bacterial populations and to a far lesser extent during growth in liquid broth. Furthermore, conjugation-proficient plasmids were able to spread infectiously in the bacterial mats initiated with various ratios of plasmid-harboring cells, resulting in a nearly exclusively plasmid-harboring population.

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“…The biosynthesis of PQQ is achieved in a series of reactions catalysed by enzymes encoded by genes located on pqq operon(s). The PQQ biosynthetic genes from several bacteria such as Acinetobacter calcoaceticus (3), Methylobacterium organophilum DSM 760 (4), Klebsiella pneumoniae (5), Pseudomonas fluorescens CHA0 (6) [reclassified as P.protegens CHA0 (7)], Methylobacterium extorquens AM1 (8), Enterobacter intermedium 60-2G (9) [reclassified as Kluyvera intermedia (10)] and Gluconobacter oxydans 621H (11) were reported and more than 125 bacterial species with PQQ biosynthetic capability were identified by bioinformatics analysis (12). Four to seven genes organized in operon(s) are responsible for PQQ biosynthesis in different bacteria (13).…”

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confidence: 99%

PqqE fromMethylobacterium extorquensAM1: a radicalS-adenosyl-l-methionine enzyme with an unusual tolerance to oxygen

et al. 2015

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Methylobacterium extorquens AM1 is an aerobic facultative methylotroph known to secrete pyrroloquinoline quinone (PQQ), a cofactor of a number of bacterial dehydrogenases, into the culture medium. To elucidate the molecular mechanism of PQQ biosynthesis, we are focusing on PqqE which is believed to be the enzyme catalysing the first reaction of the pathway. PqqE belongs to the radical S-adenosyl-L-methionine (SAM) superfamily, in which most, if not all, enzymes are very sensitive to dissolved oxygen and rapidly inactivated under aerobic conditions. We here report that PqqE from M. extorquens AM1 is markedly oxygentolerant; it was efficiently expressed in Escherichia coli cells grown aerobically and affinity-purified to near homogeneity. The purified and reconstituted PqqE contained multiple (likely three) iron-sulphur clusters and showed the reductive SAM cleavage activity that was ascribed to the consensus 2+ cluster bound at the N-terminus region. Mo¨ssbauer spectrometric analyses of the as-purified and reconstituted enzymes revealed the presence of 2+ and [2Fe-2S] 2+clusters as the major forms with the former being predominant in the reconstituted enzyme. PqqE from M.extorquens AM1 may serve as a convenient tool for studying the molecular mechanism of PQQ biosynthesis, avoiding the necessity of establishing strictly anaerobic conditions.Keywords: Methylobacterium extorquens AM1/PQQ/ PqqE/radical SAM enzyme.Abbreviations: 5 0 dA, 5 0 -deoxyadenosine; HiPIPs, high-potential iron-sulphur proteins; IPTG, isopropyl--D-thiogalactopyranoside; PQQ, pyrroloquinoline quinone; SAM, S-adenosyl-L-methionine.Pyrroloquinoline quinone (PQQ) is an aromatic, tricyclic o-quinone that serves as a cofactor for a number of prokaryotic dehydrogenases, e.g. alcohol or glucose dehydrogenase (1, 2). The biosynthesis of PQQ is achieved in a series of reactions catalysed by enzymes encoded by genes located on pqq operon(s). The PQQ biosynthetic genes from several bacteria such as Acinetobacter calcoaceticus (3), Methylobacterium organophilum DSM 760 (4), Klebsiella pneumoniae (5), Pseudomonas fluorescens CHA0 (6) [reclassified as P.protegens CHA0 (7)], Methylobacterium extorquens AM1 (8), Enterobacter intermedium 60-2G (9) [reclassified as Kluyvera intermedia (10)] and Gluconobacter oxydans 621H (11) were reported and more than 125 bacterial species with PQQ biosynthetic capability were identified by bioinformatics analysis (12). Four to seven genes organized in operon(s) are responsible for PQQ biosynthesis in different bacteria (13). In K.pneumoniae, operon pqqABCDEF is involved in PQQ production. Among six genes, only pqqA, pqqC, pqqD and pqqE are exclusively required for PQQ production (14). Despite the fact that PQQ has been found decades ago, only little is known about its biosynthetic pathway. Although the putative function of each of the genes of pqq operon(s) has been proposed based on sequence analyses and homology modelling (15), the only biochemically confirmed function is that of pqqC, which encodes a protein catalysing o...

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Phylogenetic relationships of the genus Kluyvera: transfer of Enterobacter intermedius Izard et al. 1980 to the genus Kluyvera as Kluyvera intermedia comb. nov. and reclassification of Kluyvera cochleae as a later synonym of K. intermedia

Cited by 48 publications

References 14 publications

Molecular analysis of intestinal microbiota of rainbow trout (Oncorhynchus mykiss)

Molecular analysis of intestinal microbiota of rainbow trout (Oncorhynchus mykiss)

Impact of conjugal transfer on the stability of IncP-1 plasmid pKJK5 in bacterial populations

PqqE fromMethylobacterium extorquensAM1: a radicalS-adenosyl-l-methionine enzyme with an unusual tolerance to oxygen

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