Phylogenetic relationships of Iranian<i>Allium</i>species using the<i>matK</i>(cpDNA gene) region

Zarei, Hemadollah; Fakheri, Barat Ali; Naghavi, Mohammad Reza; Mahdinezhad, Nafiseh

doi:10.5010/jpb.2020.47.1.015

Cited by 3 publications

(1 citation statement)

References 54 publications

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“…This is the case for A. vulgaris, which occupies clusters number one and two. The reason could be because matK has vast substitution rates in comparison to other chloroplast genes, and its gene sequence lies among the lowest conserved plastid genes (Zarei et al, 2020). However, in plants of different genus, there is no problem in separation for example genera Chrysanthemum and Ajania do not mix with plants from the genus Artemisia.…”

Section: Phylogenetic Treementioning

confidence: 99%

Morpho-anatomical characterization and DNA barcoding of Artemesia vulgaris L.

Wahyuni,

Indriati,

Ilham

et al. 2024

Braz. J. Biol.

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Artemisia vulgaris L. belongs to Asteraceae, is a herbal plant that has various benefits in the medical field, so that its use in the medical field can be explored optimally, the plant must be thoroughly identified. This study aims to identify A. vulgaris both in terms of descriptive morpho-anatomy and DNA barcoding using BLAST and phylogenetic tree reconstruction. The morpho-anatomical character was observed on root, stem, and leaf. DNA barcoding analysis was carried out through amplification and alignment of the rbcL and matK genes. All studies were conducted on three samples from Taman Husada (Medicinal Plant Garden) Graha Famili Surabaya, Indonesia. The anatomical slide was prepared by the paraffin method. Morphological studies revealed that the leaves of A. vulgaris both on the lower-middle part and on the upper part of the stem have differences, especially in the character of the stipules, petioles, and incisions they have. Meanwhile, from the study of anatomy, A. vulgaris has an anomocytic type of stomata and its distribution is mostly on the ventral part of the leaves. Through the BLAST process and phylogenetic tree reconstruction, the plant sequences being studied are closely related to several species of the genus Artemisia as indicated by a percentage identity above 98% and branch proximity between taxa in the reconstructed phylogenetic tree.

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Section: Phylogenetic Treementioning

confidence: 99%

Morpho-anatomical characterization and DNA barcoding of Artemesia vulgaris L.

Wahyuni,

Indriati,

Ilham

et al. 2024

Braz. J. Biol.

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Genomic profiling reveals intraspecific divergence and interspecific diversity in Allium cepa L.

Packia Lekshmi,

Brindha,

Mahamuni

et al. 2024

Genet Resour Crop Evol

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Evolution of genus is accompanied by ecological diversi cation. The majority of species grow in open, sunny, rather dry sites in arid and moderately humid climates. However, Allium species have adapted for many other ecological niches. Classical approaches for the identi cation of Allium cultivars are based on morphological traits. The assessment of these traits is di cult and their evaluation can be subjective considering that most of these cultivars are closely related. Hence, this study of Internal Transcribed Spacer (ITS) sequencing and four barcoding regions, matK, rbcL, trnH-psbA, trnL and Inter Simple Sequence Repeats (ISSR) were researched in onion, Allium cepa L. (Alliaceae) collected from three different cultivation sites. The results established noticeable hereditary divergence among the three cultivars. In ITS and matK, BDUT 1453, BDUT 1454 and BDUT 1455 were independent of each other and formed three clusters. In rbcL, BDUT 1453 formed an independent cluster from the cluster of BDUT 1454 and BDUT 1455. But in trnH-psbA, BDUT 1454 formed an independent cluster and BDUT 1453 and BDUT 1455 were closely placed whereas trnL showed all the three forming a cluster wherein BDUT 1453 and BDUT 1454 were placed closely in a sub-cluster. In ISSR, BDUT 1454 and BDUT 1455 formed a single cluster and BDUT 1453 diverged from it. Even though the tested cultivars belong to the same species they showed genetic divergence among themselves.

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Genomic Profiling Reveals Intraspecific Divergence and Interspecific Diversity in Allium cepa L.

Lekshmi,

Brindha,

Mahamuni

et al. 2023

Preprint

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Evolution of genus is accompanied by ecological diversification. The majority of species grow in open, sunny, rather dry sites in arid and moderately humid climates. However, Allium species have adapted for many other ecological niches. Classical approaches for the identification of Allium cultivars are based on morphological traits. The assessment of these traits is difficult and their evaluation can be subjective considering that most of these cultivars are closely related. Hence, this study of Internal Transcribed Spacer (ITS) sequencing and four barcoding regions, matK, rbcL, trnH-psbA, trnL and Inter Simple Sequence Repeats (ISSR) were researched in onion, Allium cepa L. (Alliaceae) collected from three different cultivation sites. The results established noticeable hereditary divergence among the three cultivars. In ITS and matK, BDUT 1453, BDUT 1454 and BDUT 1455 were independent of each other and formed three clusters. In rbcL, BDUT 1453 formed an independent cluster from the cluster of BDUT 1454 and BDUT 1455. But in trnH-psbA, BDUT 1454 formed an independent cluster and BDUT 1453 and BDUT 1455 were closely placed whereas trnL showed all the three forming a cluster wherein BDUT 1453 and BDUT 1454 were placed closely in a sub-cluster. In ISSR, BDUT 1454 and BDUT 1455 formed a single cluster and BDUT 1453 diverged from it. Even though the tested cultivars belong to the same species they showed genetic divergence among themselves.

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Phylogenetic relationships of IranianAlliumspecies using thematK(cpDNA gene) region

Cited by 3 publications

References 54 publications

Morpho-anatomical characterization and DNA barcoding of Artemesia vulgaris L.

Morpho-anatomical characterization and DNA barcoding of Artemesia vulgaris L.

Genomic profiling reveals intraspecific divergence and interspecific diversity in Allium cepa L.

Genomic Profiling Reveals Intraspecific Divergence and Interspecific Diversity in Allium cepa L.

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