Phylogenetic relationships of Brazilian bovine respiratory syncytial virus isolates and molecular homology modeling of attachment glycoprotein

Spilki, Fernando Rosado; Almeida, Renata Servan de; Domingues, Helena Gallichio; D'Arce, Regina Célia Freitas; Ferreira, Helena Lage; Campalans, Jacqueline; Costa, Sandra Cecı́lia Botelho; Arns, Clarice Weis

doi:10.1016/j.virusres.2005.08.010

Cited by 12 publications

(18 citation statements)

References 53 publications

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“…VRS has also been isolated and sequenced from bovines in countries like Brazil and Norway [18,37]. A new member of the respirovirus genus, called Caprine Parainfluenza 3 virus, was recently reported in goat herds [48]; however, to date there are no reports on the isolation or detection of PI3V or VRS specific for sheep, even though respiratory diseases in small ruminants proved to be one of the main causes of losses in the herd [25].…”

Section: Resultsmentioning

confidence: 99%

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

et al. 2017

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This is a first report in Mexico of the presence of antibodies against respiratory syncytial virus (RSV) and parainfluenza-3 virus in Mexican sheep in different productive stages. We determine the association of serological positivity with age and production system, and obtain molecular evidence of infection by both virus. RSV prevalence in adult sheep was 47% (49/105) at the tropic and 64% (63/99) at the uplands. A significant difference in RSV seropositivity between animals from the tropic and the uplands was observed (P \ 0.05). Seropositivity correlated with production system (P = 0.003, OR = 2.042), with a risk of showing antibodies was 2.042 times higher in sheep under an extensive production system. A significant difference in PI3V seropositivity between animals from either provenance (P = 0.017, OR = 0.475) were also found, with a risk of showing antibodies 0.475 times higher in sheep under an extensive production system. Genetic material from RSV and PI3V was identified by RT-PCR in nasal swab samples from clinically healthy lambs and confirmed by sequencing and phylogenetic analysis. Serological results show that sheep are susceptible to infection by both viruses, and molecular results suggest that the identified antibodies are result of natural infections and reinfections.

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Section: Resultsmentioning

confidence: 99%

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

et al. 2017

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“…É digno de nota que até o presente todos os exemplares de BRSV detectados no Brasil neste em outros trabalhos tenham se enquadrado no subgrupo B que, após ser isolado no Reino Unido nos anos 1970, nunca mais foi observado em outros países à exceção do Brasil (Nettleton et al 2003, Spilki et al 2006. As possíveis consequências práticas deste fato, em especial para a proϐilaxia da infecção, já que todas as vacinas comercializadas no país trazem vírus do subgrupo A, devem ser estudadas com experimentos controlados em bovinos ou em modelos animais (Duncan Jr et al 1993, Schrijver 1998, Spilki & Arns 2010.…”

Section: Discussionunclassified

“…A caracterização de isolados de BRSV com monoclonais e por ϐilogenia molecular permite classiϐicá-las em 3 subgrupos nomeados A, B e AB (Furze ate al., 1994). Destes, o subgrupo B foi o único relatado até o presente momento no Brasil, ainda que um número bastante reduzido de isolados esteja disponível para análise (Spilki et al 2006).…”

Section: Introductionunclassified

“…2000), proveniente dos Estados Unidos e adquirido através da American Type Culture Collection (ATCC). A amostra BRSV-25-BR e BRSV-108-BR isoladas no Laboratório de Virologia Animal/IB/Unicamp foram utilizadas como representante do subgrupo B (Arns et al 2003, Almeida et al 2005, Spilki et al 2006), e um isolado europeu cedido pelo Dr. Lars Erik Larsen (BRSV-9402022), como controle positivo do subgrupo AB (Larsen et al 1998). Todos os isolados foram propagados pela inoculação dos cultivos celulares com 0,01a 0,1 DICC 50/ mL.…”

Section: Introductionunclassified

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Detecção molecular e análise filogenética de vírus respiratório sincicial bovino (BRSV) em swabs e tecido pulmonar de bovinos adultos

2011

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Bovine respiratory syncytial viruses virus (BRSV) is one of the etiologic agents of pneumonia in young cattle. Few studies have been made aiming detection of the virus in samples collected from adult animals, especially those asymptomatic bovines. However, it is assumed that infections in these groups may occur mostly asymptomatic and this would be an important mechanism for maintaining of BRSV in herds. In this study, the goal was to conduct an analysis of the occurrence of asymptomatic infections by BRSV in lung samples (n=68) and nasal swabs (209) taken from adult animals collected in abattoirs from Southern and Southeastern Brazil respectively, to detect via polymerase chain reaction the occurrence of infected animals in populations of adult cattle. The samples that resulted positive (6) on RT-PCR were subsequently subjected to cutting with restriction enzymes and sequencing for genetic characterization (2 samples). All samples belongs to subgroup B of BRSV, which is reported as the one circulating in Brazil. The results obtained demonstrate that BRSV may be present in samples taken from adult animals, which is in agreement the hypothesis that infections in adults run in a sub-clinical way that may be of importance as a maintenance mechanism of the virus in bovine herds. RESUMO.-O vírus respiratório sincicial bovino (BRSV) é um dos agentes etiológicos de pneumonias em bovinos jovens. Poucos estudos foram realizados visando à detecção do agente em amostras coletadas de animais adultos, e em especial de bovinos assintomáticos. No entanto, presume--se que as infecções ocorridas nestes grupos possam ocorrer em sua maioria de forma assintomática e este seria um mecanismo importante para manutenção do BRSV nos rebanhos. No presente estudo, o objetivo foi realizar uma análise da prevalência de infecções assintomáticas pelo BRSV em pulmões (n=68) e swabs nasais (209) coletados de bovinos adultos coletadas em frigoríϐicos da região Sul e Sudeste respectivamente, no sentido de detectar por intermédio de reação da polimerase em cadeia qual a taxa de animais infectados em populações de animais adultos onde não ocorram sinais clínicos da infecção. As amostras positivas à RT-PCR (6) foram posteriormente submetidas ao corte com enzimas de restrição (REA) e sequenciamento para caracterização genética do gene F (2 das amostras). Todas as amostras se enquadram no subgrupo B de BRSV, o grupo circulante no Brasil conforme estudos anteriores. Os resultados obtidos demonstram que o BRSV pode estar presente em amostras obtidas de animais sadios, reforçando a hipótese de que infecções subclínicas

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“…Probably the BRSV evolution varies according to the gene, being the G protein gene highly tolerant to fixation of mutations when compared to F (VALARCHER; SCHELCHER; BOURHY, 2000). Therefore, most studies concerning characterization of isolates were focused on this protein (PROZZI et al, 1997;SCHRIJVER et al, 1998;SPILKI et al, 2006;BIDOKHTI et al, 2012). Even though, progressive accumulation of nucleotide changes was reported for fragments of both genes (VALARCHER; SCHELCHER; BOURHY, 2000).…”

Section: Introductionmentioning

confidence: 99%

Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

et al. 2014

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Bovine respiratory syncytial virus (BRSV) causes important lower respiratory tract illness in calves. According to F and G proteins genetic sequences, three BRSV subgroups have been reported and characterized in several countries, showing differences in its distribution. In Brazil, the virus is widely disseminated throughout the herds and the few characterized isolates revealed the solely occurrence of the subgroup B. This study describes the detection and characterization of an untyped BRSV strain from a twenty-days-old calf from a herd without clinical respiratory disease. Nasal swabs were analyzed by RT-nested PCR for the F and G proteins genes. One sample has amplified the F protein gene. Sequencing and subsequent phylogenetic reconstruction were accomplished, revealing that the strain could not be grouped with any other BRSV subgroups reported. This result may suggest that the BRSV is in constantly evolution, even in Brazil, where the vaccination is not a common practice. More detailed studies about BRSV characterization are necessary to know the virus subgroups distribution among the Brazilian herds to recommend appropriated immunoprophylaxis. Key words: BRSV, F protein gene, RT-nested PCR, sequences, subgroup ResumoO vírus respiratório sincicial bovino (BRSV) é responsável por causar severa doença respiratória principalmente em bezerros. De acordo com sequências genéticas das proteínas F e G deste vírus, três subgrupos de BRSV foram relatados e caracterizados em vários países, mostrando diferenças nas suas distribuições. No Brasil, o vírus encontra-se disseminado pelos rebanhos bovinos e, dos poucos

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Phylogenetic relationships of Brazilian bovine respiratory syncytial virus isolates and molecular homology modeling of attachment glycoprotein

Cited by 12 publications

References 53 publications

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

Evidence of respiratory syncytial virus and parainfluenza-3 virus in Mexican sheep

Detecção molecular e análise filogenética de vírus respiratório sincicial bovino (BRSV) em swabs e tecido pulmonar de bovinos adultos

Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

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