Phylogenetic Relationships in the Genus Rosa Revisited Based on rpl16, trnL-F, and atpB-rbcL Sequences

Abstract

<jats:p>Three chloroplast DNA (cpDNA) sequences [the <jats:italic>rpl16</jats:italic> intron, the <jats:italic>trnL-F</jats:italic>, and <jats:italic>atpB-rbcL</jats:italic> intergenic spacer (<jats:italic>IGS</jats:italic>)] were employed to study phylogenetic relationships in the genus <jats:italic>Rosa</jats:italic>. Phylogenetic analyses using these three concatenated sequences were performed using maximum parsimony (MP) and Bayesian inference (BI) methods. Both analyses results suggest that the molecular phylogeny conforms closely to the conventional classification of botanical sections. Morphological similarities between <jats:italic>R.</jats:italic> sects. <jats:italic>Synstylae</jats:italic> and <jats:italic>Chinenses</jats:italic>, and <jats:italic>R.</jats:italic> sects. <jats:italic>Rosa</jats:italic> and <jats:italic>Carolinae</jats:italic> are corroborated on the molecular level in our analyses. Four taxa from <jats:italic>R.</jats:italic> sect. <jats:italic>Pimpinellifoliae</jats:italic> are further divided into two small clades, which reflect the morphological characters for these species on a molecular level. Whereas three accessions of <jats:italic>R. foetida</jats:italic> from <jats:italic>R.</jats:italic> sect. <jats:italic>Pimpinellifoliae</jats:italic> form a separate clade. <jats:italic>R.</jats:italic> ×<jats:italic>fortuniana</jats:italic> forms a clade with <jats:italic>R. laevigata</jats:italic> based on its maternal inheritance of cpDNA. <jats:italic>R.</jats:italic> ×<jats:italic>cooperii</jats:italic> is hypothesized to be a hybrid with seed parent from <jats:italic>R.</jats:italic> sects. <jats:italic>Synstylae</jats:italic> and <jats:italic>Chinenses</jats:italic>. And <jats:italic>R. roxburghii</jats:italic> should be classified as a section within the <jats:italic>R.</jats:italic> subgen. <jats:italic>Rosa</jats:italic>, rather than being treated as its own subgenus, based on molecular analyses.</jats:p>

Comparative Analysis of the Complete Chloroplast Genome Sequences of Three Closely Related East-Asian Wild Roses (Rosa sect. Synstylae; Rosaceae)

“…The Rosa section Synstylae DC. comprises approximately 36 species, which are well-circumscribed with the distinctive synapomorphic characteristic of connate styles into an exserted column [1,5], while the phylogenetic consectionality with section Chinenses has been suggested, from several previous studies [2,5,6,7,8]. Rosa sect.…”

“…Rosa sect. Synstylae species are known to be recently diverged, based on poor resolutions and low support values from many previous molecular phylogenetic studies [2,6,7,8,10,11]. Even the most recent attempt to reconstruct the phylogeny of section Synstylae did not resolve the phylogenetic relationships with several phylogenetic problems, such as species delimitation failure, poor resolutions, and low support values, and inconsistency among gene trees [5].…”

“…In previous studies, only several chloroplast region data were used to infer the phylogeny of Rosa sect. Synstylae [5,6,7,8,10]. In this study, whole chloroplast genome sequences of three Rosa sect.…”

Comparative transcriptomics identifies patterns of selection in roses

“…Continuous flowering, fragrance, flower shape, thorn and many traits not presenting in Populus and other woody model plants could be found in roses, hence roses are now becoming a model woody species for understanding the molecular mechanisms regulating these traits. Interestingly, the breeding of modern roses often involves frequent hybridization and polyploidization among species, which often feature stronger diseases resistance and cold resistance, better fragrance and lack of prickles [ 36 – 38 ]. On the other hand, tracing the processes and history of modern roses domestication and breeding remains a challenge [ 2 ].…”

Peer Review #1 of "Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus (v0.1)"

“…Based on the HhCLA1 sequence (GenBank accession no. MK229167), the deduced protein sequence was analyzed with CLA1 proteins of other species using ClustalX (Liu et al 2015).…”