Phylogenetic Relationships between Ten genotype of Hordeum vulgare L. Using Molecular Markers

Abstract

and three landraces from Sinai (El-Kheroba, El-Sheikh Zuwaid, and Wadi Sedr) were carried out using two molecular genetic markers (RAPD-PCR and ISSR-PCR). The genetic distance between Ten genotype was also estimated from banding patterns Twenty two random primers were used in RAPD revealed 316 bands while 159 bands were detected of ISSR analysis using 10 primers. RAPD analysis among ten genotypes showed 41.77% polymorphism, while ISSR analysis showed 62.02% polymorphism. It was found that, ISSR was a more viable marker than RAPD in the detection of the genetic variability among Hordeum vulgare (barley) varieties. The genetic distance tree was detected using UPGMA based on both molecular markers (RAPD and ISSR) and analysis of combined data.In addition, the Band Shearing index (BSI) factor was calculated shows a marked difference between the ten genotypes, seven cultivars and three landraces of studied Hordeum vulgare where BSI average reach 1.42% with RAPD markers while reaching 0.93 % in ISSR. The obtained data indicated that both RAPD and ISSR markers are efficient in identification and differentiation between selected taxa, but the efficiency of ISSR was the best one. Also, the present results, enhancing the available knowledge of Hordeum vulgare genetic resources in Egypt, which may contribute to their conservation and utilization in breeding programs.

Association between Productivity, Fatty Acid Profiles, Oil Bodies’ Ultrastructure and Molecular Markers in Peanut (Arachis hypogaea L.) Cultivars

“…RAPD was also found to be an effective tool in detecting genetic diversity in peanut cultivars [18]. El Atroush et al [19] compared RAPD and ISSR's efficacy and found that both RAPD and ISSR markers are suitable tools, but ISSR is a more viable marker than RAPD in the detection of the genetic variability among Hordeum vulgare (barley) varieties. The unbiased heterozygosity (uH e ) ranged from 0.41 with the RMN-P3 primer to 0.16 with the primer OP-B18, with a mean record of 0.23 with ISSR; the uH e ranged from 0.0 with the UBC-810 primer to 0.33 with the UBC-812 and UBC-814 primers, with an average of 0.21.…”

“…They also concluded that the RAPD marker can be a significant tool in in peanut breeding programs. Phylogenetic relationships among ten Hordeum vulgare L. varieties were tested using RAPD-PCR and ISSR-PCR by El Atroush et al [19]. RAPD-PCR showed a 41.77% polymorphism, while ISSR-PCR showed a 62.02% polymorphism.…”