Phylogenetic relationships between Dicrocoelium chinensis populations in Japan and China based on mitochondrial nad1 gene sequences

Tang, Wenqiang; Ohari, Yuma; Ohtori, Maiko; Mohanta, Uday Kumar; Matsuo, Kayoko; Sato, Hiroshi; Itagaki, Tadashi

doi:10.1007/s00436-017-5557-0

Cited by 11 publications

(5 citation statements)

References 18 publications

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“…Dicrocoeliid liver flukes have previously been reported in Himalayan India, [24], China [25,26] and Iran [27,28]; but there are only anecdotal and unconfirmed reports from Pakistan [29]. The molecular methods used in the present study confirm for the first time the species identity of D. dendriticum lancet flukes collected from abattoirs in the Chitral valley.…”

Section: Discussionsupporting

confidence: 67%

Molecular confirmation of Dicrocoelium dendriticum in the Himalayan ranges of Pakistan

Khan

Afshan

Nazar

et al. 2021

Parasitology International

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Section: Discussionsupporting

confidence: 67%

Molecular confirmation of Dicrocoelium dendriticum in the Himalayan ranges of Pakistan

Khan

Afshan

Nazar

et al. 2021

Parasitology International

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“…dendriticum in sheep and goats in Pakistan’s Khyber Pakhtunkhwa and Gilgit Baltistan provinces. Dicrocoeliid liver flukes have previously been reported in India [ 43 ], China [ 44 , 45 ], Iran [ 13 ], Canada [ 5 ], France [ 16 ], Germany [ 46 ] and Italy [ 47 ]. In a small-scale study, dicrocoeliosis was recently reported in Pakistan’s Khyber Pakhtunkhwa and Gilgit Baltistan provinces [ 6 ].…”

Section: Resultsmentioning

confidence: 99%

Validation of deep amplicon sequencing of Dicrocoelium in small ruminants from Northern regions of Pakistan

Khan,

Afshan,

Firasat

et al. 2024

PLoS ONE

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Dicrocoelium lancet flukes cause significant production loss in ruminant livestock. Although co-infection with multiple Dicrocoelium species within a host is common, techniques for studying the composition of these complex parasite communities are lacking. The pathogenicity, epidemiology, and therapeutic susceptibility of different helminth species vary, and little is known about the interactions that take place between co-infecting species and their hosts. Here, we describe the first applicationof metabarcoding deep amplicon sequencing method to studythe Dicrocoelium species in sheep and goats. First, rDNA ITS-2 sequences of four Dicrocoelium species (Dicrocoelium dendriticum, Dicrocoelium hospes, Dicrocoelium orientalis, and Dicrocoelium chinensis) were extracted from the NCBI public database. Phylogenetic analysis revealed separate clades of Dicrocoelium species; hence, molecular differentiation between each species is possible in co-infections. Second, 202 flukes belonging to seventeen host populations (morphologically verified as belonging to the Dicrocoelium genus) were evaluated to determine the deep amplicon sequencing read threshold of an individual fluke for each of the four species. The accuracy of the method in proportional quantification of samples collected from single hosts was further assessed. Overall, 198 (98.01%) flukes were confirmed as D. dendriticum and 1.98% produced no reads. The comparison of genetic distances between rDNA ITS-2 revealed 86% to 98% identity between the Dicrocoelium species. Phylogenetic analysis demonstrated a distinct clustering of species, apart from D. orientalis and D. chinensis, which sit very close to each other in a single large clade whereas D. hospes and D. dendriticum are separated into their own clade. In conclusion each sample was identified as D. dendriticum based on the proportion of MiSeq reads and validated the presence of this group of parasites in the Gilgit Baltistan and Khyber Pakhtunkhwa provinces of Pakistan. The metabarcoding deep amplicon sequencing technology and bioinformatics pathway have several potential applications, including species interactions during co-infections, identifying the host and geographical distribution of Dicrocoelium in livestock, drug therapy response evaluation and understanding of the emergence and spread of drug resistance.

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“…D. dendriticum (the lancet fluke) is a small liver fluke infecting domestic animals in the Northern coasts of Africa, Asia and the Americas (Mas-Coma and Bargues, 1997 ; Traversa et al ., 2013 ; Manga-González and Ferreras, 2014 ). Using mitochondrial nd 1 and cox 1 sequences, D. dendriticum could be distinguished from D. chinensis and D. hospes (Hayashi et al ., 2017 ; Khan et al ., 2021 ). In human dicrocoeliasis, there may be true (genuine) and false (spurious) infections.…”

Section: Liver Flukesmentioning

confidence: 99%

General overview of the current status of human foodborne trematodiasis

Chai

Jung

2022

Parasitology

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Foodborne trematodes (FBT) of public health significance include liver flukes (Clonorchis sinensis, Opisthorchis viverrini, O. felineus, Fasciola hepatica and F. gigantica), lung flukes (Paragonimus westermani and several other Paragonimus spp.) and intestinal flukes, which include heterophyids (Metagonimus yokogawai, Heterophyes nocens and Haplorchis taichui), echinostomes (Echinostoma revolutum, Isthmiophora hortensis, Echinochasmus japonicus and Artyfechinostomum malayanum) and miscellaneous species, including Fasciolopsis buski and Gymnophalloides seoi. These trematode infections are distributed worldwide but occur most commonly in Asia. The global burden of FBT diseases has been estimated at about 80 million, however, this seems to be a considerable underestimate. Their life cycle involves a molluscan first intermediate host, and a second intermediate host, including freshwater fish, crustaceans, aquatic vegetables and freshwater or brackish water gastropods and bivalves. The mode of human infection is the consumption of the second intermediate host under raw or improperly cooked conditions. The major pathogenesis of C. sinensis and Opisthorchis spp. infection includes inflammation of the bile duct which leads to cholangitis and cholecystitis, and in a substantial number of patients, serious complications, such as liver cirrhosis and cholangiocarcinoma, may develop. In lung fluke infections, cough, bloody sputum and bronchiectasis are the most common clinical manifestations. However, lung flukes often migrate to extrapulmonary sites, including the brain, spinal cord, skin, subcutaneous tissues and abdominal organs. Intestinal flukes can induce inflammation in the intestinal mucosa, and they may at times undergo extraintestinal migration, in particular, in immunocompromised patients. In order to control FBT infections, eating foods after proper cooking is strongly recommended.

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Phylogenetic relationships between Dicrocoelium chinensis populations in Japan and China based on mitochondrial nad1 gene sequences

Cited by 11 publications

References 18 publications

Molecular confirmation of Dicrocoelium dendriticum in the Himalayan ranges of Pakistan

Molecular confirmation of Dicrocoelium dendriticum in the Himalayan ranges of Pakistan

Validation of deep amplicon sequencing of Dicrocoelium in small ruminants from Northern regions of Pakistan

General overview of the current status of human foodborne trematodiasis

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