Phylogenetic Relationship of Cultured and WildLabeo rohitaandCirrhinus mrigalaBased on Muscles Proteins Profile in Different Weight Groups: A New Tool in Phylogenetic Analysis

Abstract

Genetic heterogeneity and polymorphism in muscle proteins present in the meat of wild and cultured Labeo rohita and Cirrhinus mrigala was performed using SDS-PAGE. Cultured L. rohita and C. mrigala possessed more proteins than those in the wild ones and were dependent on their weight. Un-weighed pair group method with arithmetic average cluster analysis delineated a 50% linkage distance. Fish weight dissimilarity coefficient ranged from 1.73 to 3.87 in eight clusters at 75% linkage distance. The overall grouping pattern of clusters corresponded well with principal co-ordinate analysis and confirmed overall patterns of genetic variability among these species. These results provide useful guidelines for conservation and characterization of fish genetic resources for mass rearing or marker assisted breeding.

Genetic diversity and phylogenetic relationship among Anabantoidei fish (Anabantiformes) in South Kalimantan, Indonesia based on SDS-PAGE analysis

“…According to Mabrur et al (2018), the geographical origin of Channa striata has resulted some changes in protein profile of the fish, which was shown by the number of protein bands. The study of Mahboob et al (2012), the wild and cultured L. rohita and C. mrigala has shown variation on the protein profiles. Water pollution, including heavy metal contamination, industrial wastewater, and pesticide used has resulted some changes in protein expression of the fish for adaptation due to the condition.…”

“…This information obtained by protein separation based on their molecular weight using SDS-PAGE technique (Roy and Kumar 2012). Protein profiles have been used as markers for genetic diversity assessment of the freshwater fish, including snapper fish (Lutjanidae) (Velamala et al 2017), snakehead (Channidae) (Haniffa et al 2017), Oreochromis niloticus (Elghobashy et al 2005), Carp (Saxena and Soranganba 2014), Lethrinus borbonicus, Siganus rivulatus and Mulloidichthys flavolineatus (Ebied et al 2014), and Labeo rohita and Cirrhinus mrigala (Mahboob et al 2012). Therefore, this study proposed to access genetic diversity of Anabantoidei fish collected from South Kalimantan based on protein profiles obtained from SDS-PAGE analysis.…”