Phylogenetic Placement and Morphological Characterization of <i>Sclerotium rolfsii</i> (Teleomorph: <i>Athelia rolfsii</i>) Associated with Blight Disease of Ipomoea batatas in Korea

Paul, Narayan Chandra; Hwang, Eom-Ji; Nam, Sang-Sik; Lee, Hyeong-Un; Lee, Joon-Seol; Yu, Gyeong-Dan; Kang, Yong-Gu; Lee, Kyeong-Bo; Go, San; Yang, Jung‐Wook

doi:10.5941/myco.2017.45.3.129

Cited by 34 publications

(20 citation statements)

References 39 publications

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“…It causes the disease known as sclerotium rot or sclerotium wilt, widely distributed in tropical and subtropical regions (Punja and Damiani, 1996;Mahadevakumar et al, 2016). S rolfsii is known to infect several economically important crops in various stages of their growth and development, in addition to producing survival structures (Mahadevakumar et al, 2016;Paul et al, 2017;Shrestha et al, 2018). Studies conducted in Sri Lanka (Jegathambiga et al, 2010) reported the occurrence of rot symptoms in Zamioculcas sp.…”

Section: Pathogenecity Testmentioning

confidence: 99%

Soft rot on the stems of Zamioculcas zamiifolia caused by Sclerotium rolfsii

et al. 2019

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Zamioculcas zamiifolia (Araceae) is one of the most widely grown exotic species in Brazil as ornamental plants and in landscape design. Despite tolerating transport and being well adapted to low-light environments, this ornamental is attacked by different pathogens. Thus, the aim was to detect and identify the pathogen that causes stem rot in commercial Z. zamiifolia crops. Z. zamiifolia plants exhibiting stem rot symptoms were sent for phytosanitary diagnosis. In a culture medium, the fungal isolate obtained (SR-001) displayed the following morphological characteristics: cotton-like aerial mycelium, septate hyaline hyphae with no spore production, and the formation of small brown spherical sclerotia. To confirm pathogenicity, Z. zamiifolia plants were inoculated with the SR-001 isolate and, after fifteen days, the fungus was re-isolated when the same rot symptoms emerged. The SR-001 isolate was identified as Sclerotium rolfsii and its representative sequence was deposited in GenBank (Access MG694322). This fungal isolate has not been associated with diseases in Z. zamiifolia in Brazil, and this is the first report of the fungus infecting this ornamental plant species in a cultivated area.

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Section: Pathogenecity Testmentioning

confidence: 99%

Soft rot on the stems of Zamioculcas zamiifolia caused by Sclerotium rolfsii

et al. 2019

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“…Sclerotium rolfsii is an important stem and root rot pathogen of many crops (Talukder et al, 2019). This fungus is a highly polyphagous pathogen, and due to its wide host range, it is considered one of the most destructive Damping-off in Vigna subterranea and identification of associated causal agents pathogens in the world (Paul et al, 2017). The pathogen attacks around 500 plant species from 100 different botanical families (Punja, 1985;Blancard, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…The pathogen attacks around 500 plant species from 100 different botanical families (Punja, 1985;Blancard, 1988). Among its cultivated host plants are tomato (Sikirou et al, 2011), potato, cassava, chili, carrot, cabbage, peanut (Dwivedi andPrasad, 2016), cowpea (Adandonon et al, 2015), sweet potato (Paul et al, 2017), coffee, oil palm (Okabe and Matsumoto, 2000), Bambara groundnut and fruit plants (banana, citrus) (Ayandoo et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and distribution of damping-off in Vigna subterranea in Benin and identification of associated causal agents

Bossou¹,

Adandonon²,

Zoundji³

et al. 2022

Afr. Crop Sci. J.

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Bambara groundnut (Vigna subterranea (L.) Verdc.), also called ground peas or Bambara groundnut, is a West African seed legume of the Fabaceae family, playing an important social and economic role. The objective of the current study was to determine the occurrence, distribution and incidence of this disease on Bambara groundnut in agroecological production zones in Benin and to identify the causal agents responsible for the damages caused to the crop. A survey was conducted from 2019 to 2020 in five agroecological zones (AEZs) of Benin (AEZ2, AEZ3, AEZ4, AEZ5 and AEZ6). A total of 30 fields of Bambara groundnut were surveyed from 10 municipalities located in the agroeclogical zones of production at the early vegetative stage of the crop. Fields’ size of 0.5 ha were selected and scouted alongside both diagonals to evaluate the damping-off disease incidence. The results showed that damping-off occurred in four out of the five AEZs surveyed. The incidence rates varied from 0.00 to 23.33% in the AEZs 5 and 6 in 2019, and from 0.00 to 18.33% in the AEZ 6 in 2020. The highest incidence rates were obtained in the West Zone of Atacora (AEZ 4) and in the Food crop region of South (AEZ 3). Results of the correlation test showed a relationship between incidence, distribution of damping-off and climatic factors. However, most of regression equations showed a strong relationship between chemical properties of soils and incidence of damping-off. Sclerotium rolfssi was identified in the laboratory as the causal agent of the damping-off disease and isolated from diseased plants in the fields and during greenhouse pathogenicity tests. To the best of our knowledge, this is the first report of identification of pathogenic fungus as the causal agent of damping-off on Bambara groundnut seedlings in Benin.

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“…Symptoms of stem rot disease in legume plants include initially drooping of leaves close to the ground, while the branches become wilted and yellowish. Furthermore, the leaves slowly turn dark brown and sometimes fall from the plants prematurely, while the branches wither and also turn dark brown, and the lower trunk adjacent to the ground is covered with white mycelium and sclerotia growth (Karthikeyan et al, 2015;Paul et al, 2017;Nugroho et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…A. rolfsii produces an extracellular enzyme, namely cellobiose dehydrogenase (CDH) used to penetrate the host plant, while S. rolfsii also produce six extracellular enzymes, including amylase, carboxymethyl cellulase, lipase, laccase, catalase and gelatinase which are used to infect plant tissues (Chaurasia et al, 2015;Elias et al, 2015;Sennoi et al, 2021). The macroscopic and microscopic morphology of the two species are the same and difficult to distinguish, hence, molecular identification is needed to differentiate between the species (Paul et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular identification of fungi that cause stem rot disease in Bali's local legumes

Parwanayoni

Suprapta²,

Darsini³

et al. 2021

bio

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Efforts to improve food security in Indonesia, especially in Bali, need to be supported by improvements in cultivation techniques, including the management of pests and diseases. Legume crops are often attacked by stem rot diseases which can cause decreased production and economic losses. This disease is generally caused by the soil-borne pathogenic fungus Sclerotium rolfsii or Athelia rolfsii. The macroscopic and microscopic morphologies of the two species of the fungus Sclerotium rolfsii and Athelia rolfsii are the same and difficult to distinguish, so molecular identification is needed to determine the species. The research aims to isolate and molecularly identify fungi that cause stem rot disease in local legume plants in Bali. Research methods include isolation of pathogenic fungi from legumes showing symptoms of stem rot disease in Bali, pathogenicity test, identification of the highest levels of virulent isolates, DNA extraction, DNA amplification by PCR, and electrophoresis, ITS region sequencing, and computer analysis sequences DNA. The results of isolation pathogens that cause stem rot disease in Bali's local legume plants obtained six fungal isolates coded SKT, SKB1, SKB2 SKB3, SKL and SKN isolates. SKT isolates had the highest virulence rate after the pathogenicity test of peanut plants. Molecular identification results show that SKT isolate is Athelia rolfsii, because it's in a clade with Athelia rolfsii fungi sequences in GenBank with 100% Bootstrap support.

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Phylogenetic Placement and Morphological Characterization of Sclerotium rolfsii (Teleomorph: Athelia rolfsii) Associated with Blight Disease of Ipomoea batatas in Korea

Cited by 34 publications

References 39 publications

Soft rot on the stems of Zamioculcas zamiifolia caused by Sclerotium rolfsii

Soft rot on the stems of Zamioculcas zamiifolia caused by Sclerotium rolfsii

Occurrence and distribution of damping-off in Vigna subterranea in Benin and identification of associated causal agents

Isolation and molecular identification of fungi that cause stem rot disease in Bali's local legumes

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