“…Partial matK was amplified by PCR with the primers hnK-710F (Johnson and Soltis, 1995) and AST-IR (Garcia-Jacas et al, 2002). The PCR procedure included a warm start at 94OC for 1 minute 20 seconds, followed by 80°C during which the polymerase (Ecotaq, Ecogen S.R.L., Barcelona, Spain) was added, and 40 cycles of 45 seconds denaturation at 94'C, 1 minute annealing at 5S°C, 2 minutes extension at 72OC, and a final 10 minutes extension at 72OC.…”