Phylogenetic Inference in Saxifragaceae Sensu Stricto and Gilia (Polemoniaceae) Using matK Sequences

THE CARDUEAE (COMPOSITAE) REVISITED: INSIGHTS FROM ITS,trnL-trnF, ANDmatK NUCLEAR AND CHLOROPLAST DNA ANALYSIS1,2

“…Partial matK was amplified by PCR with the primers hnK-710F (Johnson and Soltis, 1995) and AST-IR (Garcia-Jacas et al, 2002). The PCR procedure included a warm start at 94OC for 1 minute 20 seconds, followed by 80°C during which the polymerase (Ecotaq, Ecogen S.R.L., Barcelona, Spain) was added, and 40 cycles of 45 seconds denaturation at 94'C, 1 minute annealing at 5S°C, 2 minutes extension at 72OC, and a final 10 minutes extension at 72OC.…”

The shrunken genome of Arabidopsis thaliana

“…The Chs primers span the last exon and were the same as used by Koch et al (1999). The primers used to amplify matK, trnK-710F and trnK-2R, originally derived from Johnson and Soltis (1995), were the same as used by Koch et al (2001). The ITS primers amplified a region that included the 5.8S region and the flanking portions of the 18S and 26S regions (Baum et al 1998;White et al 1990).…”

A phylogenetic analysis ofPfeifferaand the reinstatement ofLymanbensoniaas an independently evolved lineage of epiphyticCactaceaewithin a new tribeLymanbensonieae

“…A translation of the matK CDS reveals that only one amino acid is changed due to the inversion. Since matK is one of the fastest evolving genes in the plastid genome (Hilu & Liang 1997;Johnson & Soltis 1995), with a high proportion of substitutions even at the 1st and 2nd codon positions, changes in amino acids are relatively frequent.…”