We conducted a molecular phylogenetic study of the tribe Stipeae using nine plastid DNA sequences (trnK‐matK, matK, trnH‐psbA, trnL‐F, rps3, ndhF, rpl32‐trnL, rps16‐trnK, rps16 intron), the nuclear ITS DNA regions, and micromorphological characters from the lemma surface. Our large original dataset includes 156 accessions representing 139 species of Stipeae representing all genera currently placed in the tribe. The maximum likelihood and Bayesian analyses of DNA sequences provide strong support for the monophyly of Stipeae; including, in phylogenetic order, Macrochloa as remote sister lineage to all other Stipeae, then a primary stepwise divergence of three deep lineages with a saw‐like (SL) lemma epidermal pattern (a plesiomorphic state). The next split is between a lineage (SL1) which bifurcates into separate Eurasian and American clades, and a lineage of three parts; a small Patis (SL2) clade, as sister to Piptatherum s.str. (SL3), and the achnatheroid clade (AC). The AC exhibits a maize‐like lemma epidermal pattern throughout. AC consists of a core clade of Austral‐Eurasian distribution and a “major American clade” of North and South American distribution. The base chromosome number for Stipeae is somewhat ambiguous but based on our survey it seems most likely to be x = 11 or 12. Our phylogenetic hypothesis supports the recognition of the following genera and groups (listed by region): Eurasia—Achnatherum, “Miliacea group”, “Neotrinia” (monotypic), Orthoraphium (monotypic), Patis (also 1 from North America), Piptatherum s.str., Psammochloa (monotypic), Ptilagrostis, Stipa, “Timouria group”, and Trikeraia; Mediterranean—Ampelodesmos (monotypic), Celtica (monotypic), Macrochloa (monotypic), and “Stipella‐Inaequiglumes group”; Australasia—Anemanthele (monotypic), and Austrostipa; North America (NA)—“Eriocoma group”, Hesperostipa, Oryzopsis (monotypic), Piptatheropsis, “Pseudoeriocoma group”, and “Stillmania” (monotypic); South America—Aciachne, Amelichloa (also NA), Anatherostipa (s.str.), Jarava (polyphyletic), Lorenzochloa, Nassella (also NA), Ortachne, Pappostipa (also NA), and Piptochaetium (also NA). Monophyly of Phaenospermateae including Duthieinae is demonstrated, and its inclusion within or treatment as sister to Stipeae is rejected.
Phylogenetic analysis of ITS has been useful in identifying the major lineages in the group, and unraveling many inconsistencies in the sectional classification. However, most recent groups in the eastern Mediterranean clade are not resolved and reticulation in the western Mediterranean group of sections makes phylogenetic relationships within these two groups somewhat obscure.
a b s t r a c tWe reconstruct here the spatial and temporal evolution of the Campanula alliance in order to better understand its evolutionary history. To increase phylogenetic resolution among major groups (Wahlenbergieae-Campanuleae), new sequences from the rbcL region were added to the trnL-F dataset obtained in a previous study. These phylogenies were used to infer ancestral areas and divergence times in Campanula and related genera using a Bayesian approach to molecular dating and dispersal-vicariance analyses that takes into account phylogenetic uncertainty. The new phylogenetic analysis confirms Platycodoneae as the sister group of Wahlenbergieae-Campanuleae, the two last ones inter-graded into a well-supported clade. Biogeographic and dating analyses suggest that Western Asia and the Eastern Mediterranean have played a major role as centers of migration and diversification within the Campanula alliance, probably in relation to the intense orogenic activity that took place in this region during the Late Neogene, and that could have promoted isolation and allopatric speciation within lineages. Diversification rates within several Campanula lineages would have increased at the end of the Miocene, coinciding with the Messinian Stage. Strong selective pressures from climate changes and the expansion of mountainous regions during this period are suggested to explain the adaptation to drought, cold or disturbed environments observed in many Campanula species. Several independent long-distance dispersal events to North America are inferred within the Rapunculus clade, which seem to be related to high ploidy levels.
Taxonomic complexity has hindered the partitioning of the arti®cial genus Centaurea, even though it has long been recognized as a polyphyletic assemblage. On the basis of morphology, pollen type, karyology and DNA sequence analysis, previous workers have de®ned ®ve informal groups in the genus (Acrocentron, Centaurea sensu stricto, Cyanus, Jacea and Psephellus). However, the precise delimitation of these groups and their relationships remain largely unknown. Moreover, although some informal groups have been established among the rest of the subtribe (Amberboa, Carthamus and Stemmacantha), many genera cannot be classi®ed in any group. Newer molecular approaches are essential for resolving these problems. Sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA and the chloroplast gene matK were analysed for a comprehensive sample of the whole subtribe, with the aim of clarifying the delimitation and the phylogeny of the groups of the Centaureinae. Results largely con®rm the suggested informal entities as natural groups, with some interesting changes of placement of some genera, especially in the Acrocentron and the Stemmacantha groups. Our results con®rm that the sections of Centaurea with Dealbata pollen type should be classi®ed as a dierent genus, Psephellus. In addition to morphology and suggested pollen type evolution, the ITS and matK phylogenies are also supported by karyological evidence. Our results con®rm that the natural delimitation of Centaurea that minimizes nomenclatural changes is possible only if a new type of the genus is designated.
The Cardueae pose vexing systematic problems at several taxonomic levels. Tribal delimitation is controversial, with views ranging from a broadly defined Cardueae comprising subtribes Echinopsidinae, Carlininae, Carduinae, and Centaureinae to a more restrictive Cardueae (comprising only Carduinae and Centaureinae) with tribal rank also given to Echinopsideae (or Echinopeae) and Carlineae. Furthermore, the circumscription of groups within a broad Cardueae is also controversial, with divergent views emphasizing differences in floral or achene morphology; the distinction between Carduinae and Centaureinae is particularly weak. The generic limits of Centaurea, comprising 500–600 species and great diversity in both chromosome number and pollen morphology, are also unclear. Patterns of morphological, chromosomal, and pollen diversity in Centaurea do not completely support either the current taxonomy or any of the proposed modifications. Clearly, the Cardueae in general and Centaurea in particular require a phylogenetic approach to resolve problems of taxonomic circumscription and rank and to understand patterns of pollen and chromosomal evolution. We therefore analyzed DNA sequences of the internal transcribed spacers (ITS) of the nuclear ribosomal RNA genes (rDNA) of 35 taxa to reconstruct the phylogeny of Cardueae and assess 1) the positions of Echinopsidinae and Carlininae, 2) the circumscription of the Carduinae‐Centaureinae complex, and 3) the limits of Centaurea and related genera. Phylogenetic analysis of ITS sequence variation strongly supports the monophyly of Cardueae relative to outgroup taxa from Mutisieae and Cichorieae. Both Carlininae and Echinopsidinae occupy basal branches in Cardueae. Carduinae are paraphyletic, forming a basal grade in the tribe. The Centaureinae, however, form a well‐supported clade. As typically circumscribed, Centaurea is paraphyletic, comprising species of several other genera in addition to those currently recognized as Centaurea. Proposed evolutionary trends in pollen morphology and, to a lesser extent, base chromosome numbers are largely supported by the ITS phylogeny for Cardueae.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.