Phylogenetic diversity of bacterial endosymbionts in the gutless marine oligochete Olavius loisae (Annelida)

Dubilier, Nicole; Amann, Rudolf; Erséus, Christer; Muyzer, Gerard; Park, SueYong; Giere, Olav; Cavanaugh, Colleen M.

doi:10.3354/meps178271

Cited by 54 publications

(45 citation statements)

References 10 publications

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“…Information is emerging to show that the phylogenetic diversity of endosymbiotic bacteria of specific marine invertebrate hosts can be very wide. The gutless oligochete Olavius loisae associates with one ␥-Proteobacterium, one ␣-Proteobacterium (a novel finding), and a spirochaete (113). The endosymbionts of Pacific vent worms and bivalves include heterotrophs and chemolithotrophs, among which have been described culturable multiple-heavymetal-resistant strains (238) and uncultured filamentous ε-Proteobacteria (194).…”

Section: Diversity and Adaptationmentioning

confidence: 99%

Search and Discovery Strategies for Biotechnology: the Paradigm Shift

Bull

Ward

Goodfellow

2000

Microbiol Mol Biol Rev

416

250

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SUMMARY Profound changes are occurring in the strategies that biotechnology-based industries are deploying in the search for exploitable biology and to discover new products and develop new or improved processes. The advances that have been made in the past decade in areas such as combinatorial chemistry, combinatorial biosynthesis, metabolic pathway engineering, gene shuffling, and directed evolution of proteins have caused some companies to consider withdrawing from natural product screening. In this review we examine the paradigm shift from traditional biology to bioinformatics that is revolutionizing exploitable biology. We conclude that the reinvigorated means of detecting novel organisms, novel chemical structures, and novel biocatalytic activities will ensure that natural products will continue to be a primary resource for biotechnology. The paradigm shift has been driven by a convergence of complementary technologies, exemplified by DNA sequencing and amplification, genome sequencing and annotation, proteome analysis, and phenotypic inventorying, resulting in the establishment of huge databases that can be mined in order to generate useful knowledge such as the identity and characterization of organisms and the identity of biotechnology targets. Concurrently there have been major advances in understanding the extent of microbial diversity, how uncultured organisms might be grown, and how expression of the metabolic potential of microorganisms can be maximized. The integration of information from complementary databases presents a significant challenge. Such integration should facilitate answers to complex questions involving sequence, biochemical, physiological, taxonomic, and ecological information of the sort posed in exploitable biology. The paradigm shift which we discuss is not absolute in the sense that it will replace established microbiology; rather, it reinforces our view that innovative microbiology is essential for releasing the potential of microbial diversity for biotechnology penetration throughout industry. Various of these issues are considered with reference to deep-sea microbiology and biotechnology.

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Section: Diversity and Adaptationmentioning

confidence: 99%

Search and Discovery Strategies for Biotechnology: the Paradigm Shift

Bull

Ward

Goodfellow

2000

Microbiol Mol Biol Rev

416

250

View full text Add to dashboard Cite

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“…Several species are symbionts in hydrothermal vent invertebrates (e.g., the tube worm Riftia pachyptilia, the bivalve Calyptogena magnifica, the mussel Bathymodiolus puteoserpentis; Cavanaugh, 1994;Cavanaugh et al, 1981;Southward et al, 2001) as well as in bivalves (e.g., Thyasira sp.) and oligochetes and clams in estuarine and other environments (Dubilier et al, 1999;Krueger et al, 1996;Wood and Kelly, 1989).…”

Section: Single-cell Colorless Sulfur Bacteriamentioning

confidence: 99%

Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

Megonigal¹,

Hines²,

Visscher³

2014

Treatise on Geochemistry

128

164

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“…While the metabolism of the gamma-and alphaproteobacterial symbionts is not clear, the deltaproteobacterial symbionts have been identified as sulfate reducers and are assumed to be engaged in a syntrophic sulfur cycle with the thiotrophic symbiont (3,10). In some host species, spirochetes have also been identified as members of the oligochaete symbiont community (3,7,22).…”

mentioning

confidence: 99%

“…These symbionts are found in all host species and form a closely related cluster of 16S rRNA sequences within the Gammaproteobacteria (8). Coexisting with these primary symbionts are smaller bacteria (0.7 by 1.9 m) without any conspicuous inclusions that can belong to the Gamma-, Alpha-, or Deltaproteobacteria (3,7,10). While the metabolism of the gamma-and alphaproteobacterial symbionts is not clear, the deltaproteobacterial symbionts have been identified as sulfate reducers and are assumed to be engaged in a syntrophic sulfur cycle with the thiotrophic symbiont (3,10).…”

mentioning

confidence: 99%

Phylogeny of 16S rRNA, Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase, and Adenosine 5′-Phosphosulfate Reductase Genes from Gamma- and Alphaproteobacterial Symbionts in Gutless Marine Worms (Oligochaeta) from Bermuda and the Bahamas

Blazejak

Kuever

Erséus

et al. 2006

Appl Environ Microbiol

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Gutless oligochaetes are small marine worms that live in obligate associations with bacterial endosymbionts. While symbionts from several host species belonging to the genus Olavius have been described, little is known of the symbionts from the host genus Inanidrilus. In this study, the diversity of bacterial endosymbionts in Inanidrilus leukodermatus from Bermuda and Inanidrilus makropetalos from the Bahamas was investigated using comparative sequence analysis of the 16S rRNA gene and fluorescence in situ hybridization. As in all other gutless oligochaetes examined to date, I. leukodermatus and I. makropetalos harbor large, oval bacteria identified as Gamma 1 symbionts. The presence of genes coding for ribulose-1,5-bisphosphate carboxylase/oxygenase form I (cbbL) and adenosine 5-phosphosulfate reductase (aprA) supports earlier studies indicating that these symbionts are chemoautotrophic sulfur oxidizers. Alphaproteobacteria, previously identified only in the gutless oligochaete Olavius loisae from the southwest Pacific Ocean, coexist with the Gamma 1 symbionts in both I. leukodermatus and I. makropetalos, with the former harboring four and the latter two alphaproteobacterial phylotypes. The presence of these symbionts in hosts from such geographically distant oceans as the Atlantic and Pacific suggests that symbioses with alphaproteobacterial symbionts may be widespread in gutless oligochaetes. The high phylogenetic diversity of bacterial endosymbionts in two species of the genus Inanidrilus, previously known only from members of the genus Olavius, shows that the stable coexistence of multiple symbionts is a common feature in gutless oligochaetes.

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Phylogenetic diversity of bacterial endosymbionts in the gutless marine oligochete Olavius loisae (Annelida)

Cited by 54 publications

References 10 publications

Search and Discovery Strategies for Biotechnology: the Paradigm Shift

Search and Discovery Strategies for Biotechnology: the Paradigm Shift

Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

Phylogeny of 16S rRNA, Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase, and Adenosine 5′-Phosphosulfate Reductase Genes from Gamma- and Alphaproteobacterial Symbionts in Gutless Marine Worms (Oligochaeta) from Bermuda and the Bahamas

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