Phylogenetic comparison of the carboxy-terminal region of glycoprotein C (gC) of bovine herpesviruses (BoHV) 1.1, 1.2 and 5 from South America (SA)

Esteves, Paulo Augusto; Dellagostin, Odir A.; Pinto, Luciano S.; Silva, A.D.; Spilki, Fernando Rosado; Zanella, J. R. C.; Hübner, S.O.; Puentes, Rodrigo; Maisonnave, J.; Franco, Ana Cláudia; Rijsewijk, F.A.M.; Batista, Helena Beatriz de Carvalho Ruthner; Teixeira, Thaís Fumaco; Dezen, Diogenes; Oliveira, Anna Paula de; David, Cintia de; Arns, Clarice Weis; Roehe, Paulo Michel

doi:10.1016/j.virusres.2007.08.004

Cited by 45 publications

(39 citation statements)

References 30 publications

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“…With the advent of PCR and nucleic acid sequencing, there are many reports from other countries on the use of this technique for determination of subtypes of BHV-1 [6,11]. In the present study, the BHV-1 isolate was confirmed to be a 1.1 subtype based on PCR amplification of part of gC gene and its nucleotide sequence analysis.…”

mentioning

confidence: 51%

“…The PCR was performed using forward primer (5 0 CGGCCACGACGCTGACGA3 0 ) and reverse primer (5 0 CGCCGCCGAGTACTACCC3 0 ) (corresponding to nucleotide positions 16763-16780 and 17337-17320 of AJ004801 respectively) described by Esteves et al [6]. The 575 bp PCR product was purified by using GeneJET gel extraction kit (Fermentas, Lithuania) and the purified PCR product was cloned in pJET1.2/blunt vector by using Clone-JET PCR cloning kit (Fermentas, Lithuania).…”

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confidence: 99%

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Glycoprotein C Gene Based Molecular Subtyping of a Bovine Herpesvirus -1 Isolate from Uttar Pradesh, India

et al. 2012

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Bovine herpesvirus -1 (BHV-1) is the etiological agent of many clinical syndromes in cattle which causes huge economic losses to the animal husbandry sector annually. Since the first report of its presence in India in 1976, the disease is considered to be endemic in the country. In the present study, a case of keratoconjunctivitis in a cow was investigated to find out the underlying cause of the condition. The clinical material (ocular swab) was tested by BHV-1 glycoprotein D gene specific PCR using in house designed primers and found to be positive by the presence of a 212 bp DNA product in agarose gel electrophoresis. The virus was isolated in MDBK cell line in the third passage and the serum from the animal, was positive for antibodies against BHV-1 by ELISA. A 575 bp segment of the glycoprotein C gene of the isolate was amplified by PCR, cloned and sequenced. On phylogenetic analysis, it was seen that the sequence matched with published BHV-1.1 sequences from USA and Uruguay whereas it was divergent from Brazilian BHV-1.1 isolates. This study highlights the isolation, rapid and sensitive detection of BHV-1 virus from clinical cases and its subtyping by nucleotide sequencing and subsequent phylogenetic analysis which gives invaluable information about the molecular epidemiology of BHV-1 subtypes prevalent in the country.

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confidence: 51%

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confidence: 99%

Glycoprotein C Gene Based Molecular Subtyping of a Bovine Herpesvirus -1 Isolate from Uttar Pradesh, India

et al. 2012

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“…Detection of BoHV-1 and BoHV-5 genomes was carried out with a PCR targeting a region on the UL44 gene that codes for glycoprotein C (gC) as described by Esteves et al (2008). This non-type-discriminative reaction is expected to detect simultaneously BoHV-1 and BoHV-5.…”

Section: Methodsmentioning

confidence: 99%

“…Bovine herpesvirus 1 has been associated with respiratory tract infections (bovine infectious rinotracheitis, IBR), abortions, sporadic cases of encephalitis and reproductive tract infections (vulvovaginitis or balanopostitis) (Engels and Ackermann 1996, Muylkens et al 2007, Nandi et al 2009. Bovine herpesvirus 5 seems to be widely distributed in Latin America (Esteves et al 2008, Pidone et al 1999, Silva et al 2007, although it has been occasionally found in other regions of the world (Bartha et al 1969, Carrillo et al 1983, Diallo et al 2010, d'Offay et al 1993, Eugster et al 1975, Kirkland et al 2009. Bovine herpesvirus 5 has been associated with encephalitis and meningoencephalitis in calves and is of utmost importance in the differential diagnosis of bovine spongiform encephalitis .…”

Section: Introductionmentioning

confidence: 99%

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

Furtado¹,

Campos

Llambí³

et al. 2014

Arch. med. vet.

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“…A padronização da PCR foi realizada com a amostra padrão Shope de SuHV-1 (ATCC VR-135), amostra-padrão Colorado-1 de BoHV-1 (ATCC VR-864) e isolado EVI88/95 de BoHV-5 (Esteves et al, 2008). Devido à dificuldade de isolar o OvHV-2 , o DNA extraído de leucócitos de uma ovelha naturalmente infectada pelo OvHV-2 foi utilizado como padrão nessa etapa do trabalho (Costa et al, 2009).…”

Section: Methodsunclassified

PCR Multiplex para detecção dos principais herpesvírus neurológicos de ruminantes

Fonseca

Costa

Oliveira

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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RESUMODesenvolveu-se uma PCR multiplex (mPCR) para diagnóstico diferencial de encefalite bovina causada por herpesvírus suíno 1 (SuHV-1), herpesvírus bovino 1 (BoHV-1), herpesvírus bovino 5 (BoHV-5) e herpesvírus ovino 2 (OvHV-2). Os iniciadores foram projetados após alinhamento de sequências disponíveis no banco de genomas (GenBank) e a reação foi padronizada levando-se em consideração a concentração dos reagentes e os tipos diferentes de DNA polimerase. Após determinação da especificidade e sensibilidade, 65 amostras de encéfalo de bovinos com síndrome neurológica foram submetidas à análise. A sensibilidade analítica para detecção de BoHV-1, BoHV-5 e SuHV-1 foi, respectivamente, 10 1,2 TCID 50 /50µL, 10 1,0 TCID 50 /50µL, 10 1,3 TCID 50 /50µL na reação multiplex. Das 65 amostras analisadas, 10 foram positivas para BoHV-5, uma para BoHV-1 e cinco para OvHV-2. A mPCR descrita neste trabalho mostrou-se uma técnica útil para o diagnóstico diferencial de enfermidades relacionadas ao sistema nervoso central de bovinos. Palavras-chave: PCR, encefalite, herpesvírus ABSTRACT The aim of this study was to develop a multiplex PCR (mPCR) for the differential diagnosis of bovine encephalitis caused by the suid herpesvirus 1 (SuHV-1), bovine herpesvirus 1 (BoHV-1), bovine herpesvirus 5 (BoHV-5) and ovine herpesvirus 2 (OvHV -2). The primers were designed after alignment of sequences available in GenBank and the reaction was developed by taking into account the concentration of reagents and different types

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Phylogenetic comparison of the carboxy-terminal region of glycoprotein C (gC) of bovine herpesviruses (BoHV) 1.1, 1.2 and 5 from South America (SA)

Cited by 45 publications

References 30 publications

Glycoprotein C Gene Based Molecular Subtyping of a Bovine Herpesvirus -1 Isolate from Uttar Pradesh, India

Glycoprotein C Gene Based Molecular Subtyping of a Bovine Herpesvirus -1 Isolate from Uttar Pradesh, India

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

PCR Multiplex para detecção dos principais herpesvírus neurológicos de ruminantes

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