2011
DOI: 10.1590/s0102-09352011000600018
|View full text |Cite
|
Sign up to set email alerts
|

PCR Multiplex para detecção dos principais herpesvírus neurológicos de ruminantes

Abstract: RESUMODesenvolveu-se uma PCR multiplex (mPCR) para diagnóstico diferencial de encefalite bovina causada por herpesvírus suíno 1 (SuHV-1), herpesvírus bovino 1 (BoHV-1), herpesvírus bovino 5 (BoHV-5) e herpesvírus ovino 2 (OvHV-2). Os iniciadores foram projetados após alinhamento de sequências disponíveis no banco de genomas (GenBank) e a reação foi padronizada levando-se em consideração a concentração dos reagentes e os tipos diferentes de DNA polimerase. Após determinação da especificidade e sensibilidade, 65… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2014
2014
2019
2019

Publication Types

Select...
2

Relationship

0
2

Authors

Journals

citations
Cited by 2 publications
(1 citation statement)
references
References 32 publications
0
1
0
Order By: Relevance
“…SuHV-1 can infect many species, but pigs and wild boars are the principal hosts responsible for disease transmission [24]. Information available in the literature and in GenBank indicated the type of animals from which the viruses were isolated, but we could not find any correlation between clusters, genetic variability, and species (data not shown).…”
Section: Discussionmentioning
confidence: 71%
“…SuHV-1 can infect many species, but pigs and wild boars are the principal hosts responsible for disease transmission [24]. Information available in the literature and in GenBank indicated the type of animals from which the viruses were isolated, but we could not find any correlation between clusters, genetic variability, and species (data not shown).…”
Section: Discussionmentioning
confidence: 71%