Phylogenetic characterization and virulence of two Newcastle disease viruses isolated from wild birds in China

Liu, Haijin; Zhang, Peng; Wu, Pengpeng; Chen, Shengli; Mu, Guohui; Duan, Xuji; Hao, Hiroyuki; Wang, Jianxiao; Wang, Xinglong; Yang, Zhicong

doi:10.1016/j.meegid.2013.08.021

Cited by 17 publications

(9 citation statements)

References 79 publications

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“…Therefore, constant monitoring is required to elucidate the role of natural reservoir in the ecology of NDV. Our previous study described the pathogenic and phylogenetic characteristics of several wild bird NDVs, such as Shaanxi06 (velogenic, genotype VIId), Shaanxi10 (mesogenic, genotype VIi), Spotted-dove08 (letogenic, genotype IX) and Blackbird08 (velogenic, genotype IX) (Chen et al, 2013;Liu et al, 2013). In the present study, an NDV strain was isolated from a pigeon in China.…”

Section: Introductionmentioning

confidence: 83%

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Molecular characterization of a Class I Newcastle disease virus strain isolated from a pigeon in China

et al. 2016

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Constant monitoring is performed to elucidate the role of natural hosts in the ecology of Newcastle disease virus (NDV). In this study, an NDV strain isolated from an asymptomatic pigeon was sequenced and analysed. Results showed that the full-length genomes of this isolate were 15,198 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. This NDV isolate was lentogenic, with an intracerebral pathogenicity index of 0.00 and a mean time of death more than 148 h. The isolate possessed a motif of -(112)E-R-Q-E-R-L(117)- at the F protein cleavage site. In addition, 7 and 13 amino acid substitutions were identified in the functional domains of fusion protein (F) and haemagglutinin-neuraminidase protein (HN) proteins, respectively. Analysis of the amino acids of neutralizing epitopes of F and HN proteins showed 3 and 10 amino acid substitutions, respectively, in the isolate. Phylogenetic analysis classified the isolate into genotype Ib in Class I. This isolate shared high homologies with the NDV strains isolated from wild birds and waterfowl in southern and eastern parts of China from 2005 to 2013. To our knowledge, this study is the first to report a NDV strain isolated from pigeon that belongs to genotype Ib in Class I, rather than to the traditional genotype VI or other sub-genotypes in Class II. This study provides information to elucidate the distribution and evolution of Class I viruses for further NDV prevention.

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Section: Introductionmentioning

confidence: 83%

“…The NDV strain used in this study is indicated by a filled black circle. NDV strains used in Class I and Class II are referred to in the literature (Kim et al, 2007;Diel et al, 2012;Liu et al, 2013;Fan et al, 2015;Wu et al, 2015).…”

Section: Phylogenetic Tree and Evolutionary Distance Analysesmentioning

confidence: 99%

Molecular characterization of a Class I Newcastle disease virus strain isolated from a pigeon in China

et al. 2016

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“…Based on this observation, it is possible that wild and pet birds may play an important link for infections reported in chickens, although intensive surveillance and NDV monitoring in wild birds will be necessary to prove this observation. However, several positive reports have already been published testing the theory that wild birds act as carriers for NDV (Kim et al., ; Lindh et al., ; Cardenas Garcia et al., ; Liu et al., ; Duan et al., ). The presence of water routes connecting these countries supports the notion that diverse NDV viruses are travelling across countries via the medium of wild migratory birds.…”

Section: Discussionmentioning

confidence: 99%

Pathotypic and Sequence Characterization of Newcastle Disease Viruses from Vaccinated Chickens Reveals Circulation of Genotype II, IV and XIII and in India

Jakhesara

Prasad

Pal

et al. 2014

Transbound Emerg Dis

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Newcastle disease virus (NDV) causes a highly contagious disease which continuously haunts the global poultry industry. The nature and molecular epidemiology of NDVs prevalent in recent outbreaks in India is poorly understood. This study aimed to characterize NDVs prevalent in vaccinated flocks in India using whole-genome sequencing and biological pathotyping. Twelve field isolates were collected from outbreaks which occurred in different parts of India and characterized as velogenic based on their intracerebral pathogenicity index (ICPI) and amino acid sequence at the F protein cleavage site. All 12 of the field isolates and five commonly used vaccine strains were selected for whole-genome sequencing using Ion Torrent PGM technology, yielding complete genome sequences for ten field isolates and all vaccine strains. The genome of all isolates was found to be 15 192 nt long with a high level of conservation across multiple genomic features with APMV-I viruses. Phylogenetic analysis and evolutionary distance calculations placed the isolates in genotypes II, IV and XIII. Revisiting other recently reported strains provided preliminary evidence of genotypes VI, VII and XVIII circulating in India. Comparison between the field and vaccine virus sequences revealed unique genomic and amino acid differences in important antigenic regions of the F and hemagglutinin-neuraminidase (HN) genes which can be targeted for site directed mutagenesis to evaluate the impact of these substitutions on virus pathogenicity. This study highlights the requirement to evaluate current vaccines through systematic protection assays to determine protection efficacy against field isolates.

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“…In the USA, virulent NDV was isolated from gulls and cormorants in the state of Maine, Maryland, New Hampshire, Minnesota and Massachusetts (Diel et al., ). Newcastle disease virus isolation was also reported from a mosquito pool in Jakarta and also from a wild bird population which included mallards and spotted‐necked dove (Forrester et al., ; Liu et al., ; Tolf et al., ).…”

Section: Avian Paramyxovirus Serotype‐1mentioning

confidence: 99%

Avian Paramyxovirus: A Brief Review

Gogoi

Ganar

Kumar

2015

Transbound Emerg Dis

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Avian paramyxoviruses (APMVs) have been reported from a wide variety of avian species around the world. Avian paramyxoviruses are economically significant because of the huge mortality and morbidity associated with it. Twelve different serotypes of APMV have been reported till date. Avian paramyxoviruses belong to the family Paramyxoviridae under genus Avulavirus. Newcastle disease virus (APMV-1) is the most characterized members among the APMV serotypes. Complete genome sequence of all twelve APMV serotypes has been published recently. In recent years, APMV-1 has attracted the virologists for its oncolytic activity and its use as a vaccine vector for both animals and humans. The recombinant APMV-based vaccine offers a pertinent choice for the construction of live attenuated vaccine due to its minimum recombination frequency, modular nature of transcription and lack of DNA phase during its replication. Although insufficient data are available regarding other APMV serotypes, our understanding about the APMV biology is expanding rapidly because of the availability of modern molecular biology tools and high-throughput complete genome sequencing.

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Phylogenetic characterization and virulence of two Newcastle disease viruses isolated from wild birds in China

Cited by 17 publications

References 79 publications

Molecular characterization of a Class I Newcastle disease virus strain isolated from a pigeon in China

Molecular characterization of a Class I Newcastle disease virus strain isolated from a pigeon in China

Pathotypic and Sequence Characterization of Newcastle Disease Viruses from Vaccinated Chickens Reveals Circulation of Genotype II, IV and XIII and in India

Avian Paramyxovirus: A Brief Review

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