Phylogenetic background and carriage of pathogenicity island-like domains in relation to antibiotic resistance profiles among Escherichia coli urosepsis isolates

Houdouin, Véronique; Bonacorsi, Stéphane; Bidet, Philippe; Bingen-Bidois, Martine; Barraud, Dominique; Bingen, Édouard

doi:10.1093/jac/dkl326

Cited by 34 publications

(43 citation statements)

References 32 publications

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“…iutA was present in 84.9% of the susceptible isolates and 80.6% of the resistant ones. The prevalence was high among the investigated strains, compared to 79% reported by Houdouin et al (Houdouin et al, 2006), while all other studies reported lower percentages (Johnson et al, 2005;Soto et al, 2007). Such differences might be due to differences in association with host characteristics.…”

Section: Discussionmentioning

confidence: 51%

Virulence Genotype and Phylogenetic Groups in relation to Chinese Herb Resistance among <i>Escherichia Coli</i> from Patients with acute Pyelonephritis

Tong¹,

Sun²,

Chen³

2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Clinical isolates of herb-resistant uropathogenic E. coli were isolated. It was possible that the virulence genotypes and phylogenetic background of E. coli differed between Chinese herb-resistant E. coli and -susceptible isolates. For this purpose, the prevalence of virulence factors (VFs) and phylogenetic background, with regard to Chinese herb resistance, among E. coli strains causing acute pyelonephritis from China were investigated. Materials and Methods:E. coli isolates from patients with acute pyelonephritis were used in this study. Standard disc diffusion methodology was used to test the susceptibility of Chinese herbal concoction against E. coli strains. Multiplex PCR amplifications employed three markers (chuA, yjaA, and TSPE4.C2) to classify E. coli isolates into one of four phylogenetic groups (group A, B1, B2, or D). The isolates were also tested for 14 virulence-associated traits (VFs) of uropathogenic E. coli.Results: A total of 115 E. coli strains were isolated. 79 (68.7%) were susceptible and 36 (31.3%) were resistant to the herbal concoction. 20.9% of the isolates encoded three or more of VFs for which they were screened, with 13.9% in susceptible isolates and 36.1% in resistant isolates. The key VFs (fyuA and/or iutA siderophores) present in >80% of isolates. The papA and papC adhesins were detected in the majority of resistant isolates (72.2% and 63.9% respectively). 78.5% of susceptible isolates belong to phylogenetic groups A, while 83.3% of resistant isolates belong to group B2. Conclusion:PapA and papC are significant VFs with an essential role in contributing to Chinese herb-resistance. Chinese herb-resistance is associated with a shift towards more virulent strains and B2 phylogenetic group.

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Section: Discussionmentioning

confidence: 51%

Virulence Genotype and Phylogenetic Groups in relation to Chinese Herb Resistance among <i>Escherichia Coli</i> from Patients with acute Pyelonephritis

Tong¹,

Sun²,

Chen³

2014

Afr. J. Trad. Compl. Alt. Med.

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“…A simple phylogenetic scheme based on the presence or absence of a few selected genes has been used to demonstrate genetic differences between intestinal E. coli and ExPEC (Clermont et al, 2000). Several studies have noted the predominance of phylogenetic groups B2 and D amongst UPEC isolates Houdouin et al, 2006;Sabaté et al, 2006;Bidet et al, 2007) or meningitis isolates (Bidet et al, 2007;Ewers et al, 2007). However, few studies have sought to characterize E. coli isolates associated with IBD or colon cancer.…”

Section: Discussionmentioning

confidence: 99%

A subset of mucosa-associated Escherichia coli isolates from patients with colon cancer, but not Crohn's disease, share pathogenicity islands with urinary pathogenic E. coli

et al. 2008

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“…Prevalence of phylogroup B2 was observed among the isolates susceptible to ampicillin, ciprofloxacin and trimethoprim/sulfamethoxazole, supporting the old paradigm of a trade-off between resistance and virulence (Johnson et al, 2003(Johnson et al, , 2005Houdouin et al, 2006;Moreno et al, 2006;Piatti et al, 2008). Nonetheless, the majority of the isolates resistant to ampicillin and ciprofloxacin also belong to phylogroup B2.…”

Section: Discussionmentioning

confidence: 64%

“…This confirms that the trade-off between resistance and virulence is also verified for the PAIs considered to be more unstable. Previous studies have already demonstrated that the resistance to quinolones is associated with a lower prevalence of PAI II J96 -like domains (Houdouin et al, 2006;Piatti et al, 2008).…”

Section: Discussionmentioning

confidence: 92%

Interplay between pathogenicity island carriage, resistance profile and plasmid acquisition in uropathogenic Escherichia coli

Calhau

Domingues

Ribeiro³

et al. 2015

Journal of Medical Microbiology

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This study aimed to characterize the relationship between pathogenicity islands (PAIs), single virulence genes and resistance among uropathogenic Escherichia coli, evaluating the resistance plasmid carriage fitness cost related to PAIs. For 65 urinary E. coli, antimicrobial susceptibility and extended-spectrum b-lactamase production were determined with the Vitek 2 Advanced Expert system. Phylogroup determination, detection of PAIs and virulence genes papAH, papC, sfa/foc, afa/dra, iutA, kpsMII, cnf1, eaeA, hlyA, stx1 and stx2, plasmid replicon typing and screening for plasmidic resistance determinants qnr, aac(69)-Ib-cr, qepA and bla CTX-M were carried out by PCR. Conjugation was performed between a donor carrying IncF, IncK and bla CTX-M-15 , and receptors carrying one to six PAIs. The relative fitness of transconjugants was estimated by pairwise competition experiments. PAI IV 536 (68 %), gene iutA (57 %) and resistance to ampicillin were the most prevalent traits. PAI I 536 , PAI II 536 , PAI III 536 and PAI II J96 were exclusively associated with susceptibility to amoxicillin/clavulanic acid, cefotaxime, ceftazidime, ciprofloxacin, gentamicin and trimethoprim/sulfamethoxazole, and were more prevalent in strains susceptible to ampicillin and cefalotin. PAI IV 536 , PAI II CFT073 and PAI I CFT073 were more prevalent among isolates showing resistance to amoxicillin/clavulanic acid, cefalotin, cefotaxime, ceftazidime and gentamicin. An inverse relationship was observed between the number of plasmids and the number of PAIs carried. Transconjugants were obtained for receptors carrying three or fewer PAIs. The mean relative fitness rates of these transconjugants were 0.87 (two PAIs), 1.00 (one PAI) and 1.09 (three PAI). The interplay between resistance, PAI carriage and fitness cost of plasmid acquisition could be considered PAI specific, and not necessarily associated with the number of PAIs.

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Phylogenetic background and carriage of pathogenicity island-like domains in relation to antibiotic resistance profiles among Escherichia coli urosepsis isolates

Cited by 34 publications

References 32 publications

Virulence Genotype and Phylogenetic Groups in relation to Chinese Herb Resistance among <i>Escherichia Coli</i> from Patients with acute Pyelonephritis

Virulence Genotype and Phylogenetic Groups in relation to Chinese Herb Resistance among <i>Escherichia Coli</i> from Patients with acute Pyelonephritis

A subset of mucosa-associated Escherichia coli isolates from patients with colon cancer, but not Crohn's disease, share pathogenicity islands with urinary pathogenic E. coli

Interplay between pathogenicity island carriage, resistance profile and plasmid acquisition in uropathogenic Escherichia coli

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