Virulence Genotype and Phylogenetic Groups in relation to Chinese Herb Resistance among &lt;i&gt;Escherichia Coli&lt;/i&gt; from Patients with acute Pyelonephritis

Tong, YanQing; Sun, Song; Chen, Ying

doi:10.4314/ajtcam.v11i3.33

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…The other 41.13% could not be typed as ExPEC using this method, indicating that the predictive virulence genes score is not always sufficient for classification of ExPEC as has also been reported before (Berman et al, 2014 ). In general, ExPEC can be classified into five phylogenetic groups, i.e., A, B (subgroups B1 and B2), D, E, and F, and the majority of the isolates in our study belonged to phylogenetic groups B2 and D. Indeed, other studies, as the ones from Iran and China, show that human pathogenic ExPEC predominantly belong to these two groups (Kazemnia et al, 2014 ; Tong et al, 2014 ), that are also considered to be more virulent and more associated with infections than, e.g., phylogenetic groups A and B1 (Lee et al, 2016 ). In our study, two isolates could not be assigned to any of the phylogenetic groups.…”

Section: Discussionsupporting

confidence: 54%

Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil

et al. 2018

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Urinary tract infections (UTIs) are often caused by Escherichia coli. Their increasing resistance to broad-spectrum antibiotics challenges the treatment of UTIs. Whereas, E. coli ST131 is often multidrug resistant (MDR), ST69 remains susceptible to antibiotics such as cephalosporins. Both STs are commonly linked to community and nosocomial infections. E. coli phylogenetic groups B2 and D are associated with virulence and resistance profiles making them more pathogenic. Little is known about the population structure of E. coli isolates obtained from urine samples of hospitalized patients in Brazil. Therefore, we characterized E. coli isolated from urine samples of patients hospitalized at the university and three private hospitals in Rio de Janeiro, using whole genome sequencing. A high prevalence of E. coli ST131 and ST69 was found, but other lineages, namely ST73, ST648, ST405, and ST10 were also detected. Interestingly, isolates could be divided into two groups based on their antibiotic susceptibility. Isolates belonging to ST131, ST648, and ST405 showed a high resistance rate to all antibiotic classes tested, whereas isolates belonging to ST10, ST73, ST69 were in general susceptible to the antibiotics tested. Additionally, most ST69 isolates, normally resistant to aminoglycosides, were susceptible to this antibiotic in our population. The majority of ST131 isolates were ESBL-producing and belonged to serotype O25:H4 and the H30-R subclone. Previous studies showed that this subclone is often associated with more complicated UTIs, most likely due to their high resistance rate to different antibiotic classes. Sequenced isolates could be classified into five phylogenetic groups of which B2, D, and F showed higher resistance rates than groups A and B1. No significant difference for the predicted virulence genes scores was found for isolates belonging to ST131, ST648, ST405, and ST69. In contrast, the phylogenetic groups B2, D and F showed a higher predictive virulence score compared to phylogenetic groups A and B1. In conclusion, despite the diversity of E. coli isolates causing UTIs, clonal groups O25:H4-B2-ST131 H30-R, O1:H6-B2-ST648, and O102:H6-D-ST405 were the most prevalent. The emergence of highly virulent and MDR E. coli in Brazil is of high concern and requires more attention from the health authorities.

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Section: Discussionsupporting

confidence: 54%

Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil

et al. 2018

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“…Consistent with our study, a study from Egypt 35 has documented that phylogroup A is predominant in UPEC strains followed by group B2 and lastly D. The predominance of phylogroup A E. coli has been also reported in China, 39 Russia 40 and Iran. 41…”

Section: Discussionsupporting

confidence: 91%

“…On the other hand, phylogroup D was the most prevalent group causing UTI in China. 38 Consistent with our study, a study from Egypt 35 has documented that phylogroup A is predominant in UPEC strains followed by group B2 and lastly D. The predominance of phylogroup A E. coli has been also reported in China, 39 Russia 40 and Iran. 41 It has been suggested that phylogroup A could be pathogenic as other E. coli phylogroups as it possess the virulence genes as other pathogenic phylogroups.…”

Section: Discussionsupporting

confidence: 90%

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

El-Mahdy

Mahmoud

Shrief

2021

IDR

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Purpose Characterization of different uropathogenic E. coli (UPEC) phylogroups is crucial to understand pathogenesis of urinary tract infection (UTI). The objective of our study was to evaluate the antibiotic resistance pattern, biofilm formation and pathogenicity islands (PAIs) of UPEC phylogroups isolated from catheter-associated UTI (CAUTI) compared to community UTI (Com-UTI). Patients and Methods This study included 90 UPEC strains recovered from CAUTI and Com-UTI. Antimicrobial susceptibility was tested by the Kirby–Bauer method and extended spectrum beta-lactamase (ESBL) production was confirmed using the combined disk. The biofilm formation was tested using the microtiter plate assay. Main E. coli phylogroups (A, B1, B2 and D) were detected by multiplex PCR and 2 multiplex PCR detected the 8 PAIs. Results Antibiotic resistance of UPEC strains showed a similar high resistance in CAUTI and Com-UTI. Isolates from CAUTI significantly produced biofilm higher than Com-UTI strains (68.9% vs 44.4%). In CAUTI and Com-UTI isolates, phylogroup A was the commonest (53.3% vs 48.9%, respectively). PAI IV536 was the most common in the strains from CAUTI (71.1%) and Com-UTI (73.3%). No significant relationship was detected between the studied characters and different phylogroups except the significant resistance to cefotaxime, ceftazidime and aztreonam among phylogroups from CAUTI isolates. Conclusion Increased antibiotic resistance and ESBLs were detected in UPEC strains from CAUTI and Com-UTI. The strains from CAUTI significantly produced biofilm higher than Com-UTI strains. Phylogroup A was the predominate phylogroup and PAI IV536 was the most prevalent marker in all phylogroups from both types of UTI.

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“…The predominance of phylogenetic group A in UPEC isolates which is usually associated with commensal strains suggesting that the gastrointestinal tract is the main source of strains that colonize the urinary tract [32, 33]. The distribution of virulence genes and phylogenetic types varies among different countries, for example, group A was the most prevalent in Russia [34], and also in China [35] in UPEC, so our findings can be explained by geographical variation.…”

Section: Discussionmentioning

confidence: 84%

Phylogenic classification and virulence genes profiles of uropathogenic E. coli and diarrhegenic E. coli strains isolated from community acquired infections

et al. 2019

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The emergence of E.coli strains displaying patterns of virulence genes from different pathotypes shows that the current classification of E.coli pathotypes may be not enough, the study aimed to compare the phylogenetic groups and urovirulence genes of uropathogenic Escherichia coli (UPEC) and diarrheagenic E.coli (DEC) strains to extend the knowledge of E.coli classification into different pathotypes. A total of 173 UPEC and DEC strains were examined for phylogenetic typing and urovirulence genes by PCR amplifications. In contrast to most reports, phylogenetic group A was the most prevalent in both UPEC and DEC strains, followed by B2 group. Amplification assays revealed that 89.32% and 94.29% of UPEC and DEC strains, respectively, carried at least one of the urovirulence genes, 49.5% and 31.4% of UPEC and DEC strains, respectively, carried ≥ 2 of the urovirulence genes, fim H gene was the most prevalent (66.9% and 91.4%) in UPEC and DEC strains respectively. Twenty different patterns of virulence genes were identified in UPEC while 5 different patterns in DEC strains. Strains with combined virulence patterns of four or five genes were belonged to phylogenetic group B2. Our finding showed a closer relationship between the DEC and UPEC, so raised the suggestion that some DEC strains might be potential uropathogens. These findings also provide different insights into the phylogenetic classification of E. coli as pathogenic or commensals where group A can be an important pathogenic type as well as into the classification as intestinal or extra- intestinal virulence factors.

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Virulence Genotype and Phylogenetic Groups in relation to Chinese Herb Resistance among <i>Escherichia Coli</i> from Patients with acute Pyelonephritis

Cited by 8 publications

References 14 publications

Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil

Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil

Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

Phylogenic classification and virulence genes profiles of uropathogenic E. coli and diarrhegenic E. coli strains isolated from community acquired infections

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