Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China

Abstract

Two velogenic Newcastle disease viruses (NDV) obtained from outbreaks in domestic ducks in China were characterized in this study. Phylogenetic analysis revealed that both strains clustered with the class II viruses, with one phylogenetically close to the genotype VII NDVs and the other closer to genotype IX. The deduced amino acid sequence of the cleavage site of the fusion (F) protein confirmed that both isolates contained the virulent motif 112RRQK/RRF117 at the cleavage site. The two NDVs had severe pathogenicity in fully susceptible chickens, resulting in 100% mortality. One of the isolates also demonstrated some pathogenicity in domestic ducks. The present study suggests that more than one genotype of NDV circulates in domestic ducks in China and viral transmission may occur among chickens and domestic ducks.

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

“…Moreover, it was revealed that our isolate is closely related with isolates of NARC-Pak, Israel, Kudus and Sragen, while it was distantly related with isolates of Iran, Guangdong, Japan, India and USA. [5,[26][27] did epidemiological investigations of NDV by phylogenetic analysis using a partial nucleotide sequence of a fusion protein gene. Our findings are in line with their results.…”

Çin’de Evcil Ördeklerden İzole Edilen Newcastle Hastalığı Viruslarının Genotipik ve Patojenik Karakterizasyonu

“…The birds mostly harbor lentogenic NDVs, the strains apparently have the potential to become velogenic after transmission and circulation, and the virulence of these viruses has increasing trends. In ducks, many NDVs have been isolated in different country in recent years, studies on genetic diversity among the isolates revealed that most of the NDVs belong to class I with low virulence, occasionally a velogenic strain is isolated [15,[22][23][24] . Due to the lack of surveillance, little is known about distribution, pathogenic and molecular characterizations of NDV in domestic ducks.…”

“…Most of isolates are lentogenic and have not lead to great losses to duck farms. Occasionally when a velogenic strain is isolated, it is particularly urgent to evaluate potential risk to domestic ducks [14,15] . While there have insufficient knowledge of pathogenic characterizations and molecular analyses of NDV isolated from domestic ducks in China, and we also have limited knowledge of the relationship between the Chinese representative NDV isolated from chickens and the strains isolated recently from domestic ducks in China.…”

<b>Detecção e caracterização moleculares dos paramixovírus aviários tipo 1 em materiais provenientes de aves silvestres utilizando testes para a detecção dos vírus da família viral Paramyxoviridae</b>

“…Estes vírus são classificados como lentogênicos e velogênicos e causam infecções em aves domésticas e silvestres (DIMITROV et al, 2016 O genotipo IX tem sido detectado principalmente na China (ZHANG et al, 2011).…”

“…Todos os vírus deste genotipo são classificados como velogênicos pelo sequenciamento gênico (ZHANG et al, 2011), embora alguns isolados sejam classificados como lentogênicos pelo teste de patogenicidade in vivo (DUAN et al, 2014). Este vírus são detectados em aves domésticas e silvestres assintomáticas (DUAN et al, 2014).…”