Phylogenetic analysis using E2 gene of classical swine fever virus reveals a new subgenotype in China

Jiang, Donald Dah-Shyong; Gong, Wenjie; Li, Run-Cheng; Li, Guohua; Hu, Yunfei; Ge, Meng; Wang, Shuqin; X, Yu; Tu, Changchun

doi:10.1016/j.meegid.2013.04.004

Cited by 36 publications

(31 citation statements)

References 21 publications

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“…We sequenced the PCR product using Sanger's sequencing method (Jiang et al . ). We constructed a phylogenetic tree which included the E2 sequence of our porcine strain (accession no.…”

Section: Case Reportmentioning

confidence: 97%

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An outbreak of classical swine fever in pigs in Bangladesh, 2015

Sarkar

Hossain

Gurley

et al. 2017

Veterinary Medicine & Sci

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In a group of 22 healthy pigs aged between 4 and 6 months, 2 pigs became ill with high fever, complete anorexia, cough and abnormal swaying movements on 22 June 2015. One of them died on June 24 and the second died on July 3. Shortly after, the remaining pigs also fell ill and died from the same illness by 10 August 2015. We investigated the aetiology, epidemiological and clinical features of the outbreak. We recorded the clinical signs and symptoms for each pig with the date of onset of illness. Veterinarians conducted post‐mortem examinations on the 12 dead pigs, they collected tissue samples from the dead pigs and placed them in a tube containing 1 mL of nucleic acid extraction buffer (lysis buffer). We tested all the tissue samples by real‐time reverse transcription polymerase chain reaction (rRT‐PCR) to detect classical swine fever virus (CSFV) because the animals’ symptoms matched those of this disease. We also conducted a phylogentic analysis of the nucleotide sequence of the E2 gene segment of CSFV detected in a lung tissue sample. The attack rate (22/22) and the case fatality were 100%. The predominant symptoms of the disease included high fever, cough, diarrhoea and swaying movements of the hind legs prior to death. Of the 12 pigs tissue samples tested, all had evidence of the presence of CSFV RNA by rRT‐PCR. The phylogenetic analysis indicated that the virus belongs to genotype 2.2, which is closely related to CSFV genotype 2.2 reported in India. Our investigation suggests that CSF is circulating in pigs, posing a risk for communities in Bangladesh that rely on pigs for economic income and dietary protein. Future research could focus on estimating the disease and economic burden of CSFV in pig rearing areas to determine if interventions might be warranted or cost‐effective.

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“…We sequenced the PCR product using Sanger's sequencing method (Jiang et al . ). We constructed a phylogenetic tree which included the E2 sequence of our porcine strain (accession no.…”

Section: Case Reportmentioning

confidence: 97%

“…We prepared cDNA and subsequently performed PCR to amplify a 1342-bp segment of the E2 gene of CSFV. We sequenced the PCR product using Sanger's sequencing method (Jiang et al 2013). We constructed a phylogenetic tree which included the E2 sequence of our porcine strain (accession no.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

An outbreak of classical swine fever in pigs in Bangladesh, 2015

Sarkar

Hossain

Gurley

et al. 2017

Veterinary Medicine & Sci

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“…In mainland China, CSFV isolates circulating in the late 1980s and 1990s consisted of four subgenotypes (1.1, 2.1, 2.2, and 2.3) (5). More recently, the genetic diversity of subgenotype 2.1 isolates was analyzed, and 10 sub-subgenotypes (2.1a to 2.1j) have been identified (6, 7). A greater understanding of the mechanisms of emergence of subgenotype 2.1 isolates will require analysis of additional complete genomes.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Sub-Subgenotype 2.1i Isolate of Classical Swine Fever Virus from China

Zhang

Bao

et al. 2017

Genome Announc

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“…The bootstrap values were calculated with the modules SEQBOOT, DNADIST, NEIGHBOR and CON-SENSE of the PHYLIP Ver. 3.59 package [8]. The phylogenetic tree, constructed by using the neighbour-joining method, were computed with the DNADIST, NEIGH-BOUR modules with the same parameters.…”

Section: Virus Isolation and Rt-pcrmentioning

confidence: 99%

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

et al. 2015

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The phylogenetic analysis of 11 CSFV isolates from Karnataka, India obtained during the year 2012-13 was undertaken to obtain the most reliable genetic typing of the CSFV isolates based on E2, NS5B and 5 0 UTR genomic regions. The study indicated that all the 11 CSFV isolates belonged to subgroup 2.2. The most reliable classification was obtained with sequence data from the NS5B region which separated all the isolates based on the history of outbreak and geographic origin. Analysis of full length E2 amino acid sequences revealed different genetic makeup of Indian 2.2 isolates compared to 2.2 isolates from different countries. The group 2.2 viruses are gradually spreading as confirmed by frequent detection/ isolation of group 2.2 viruses in the recent years and replacing the subgroup 1.1 viruses, which were hitherto predominantly involved in CSF outbreaks in India.Keywords Classical swine fever virus Á Phylogenetic analysis Á E2 Á NS5B and 5 0 UTR

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Phylogenetic analysis using E2 gene of classical swine fever virus reveals a new subgenotype in China

Cited by 36 publications

References 21 publications

An outbreak of classical swine fever in pigs in Bangladesh, 2015

An outbreak of classical swine fever in pigs in Bangladesh, 2015

Complete Genome Sequence of a Sub-Subgenotype 2.1i Isolate of Classical Swine Fever Virus from China

Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1

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