Phylogenetic analysis of the E2 gene of classical swine fever virus from the Guangxi Province of southern China

Luo, Ting; Liao, Su-Huan; Wu, Xianshi; Feng, Liang; Yuan, Zhaoxia; Li, Hui; Liang, Jingjing; Meng, Xiangzhi; Zhang, Hongyun

doi:10.1007/s11262-011-0578-8

Cited by 23 publications

(18 citation statements)

References 24 publications

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“…From Nepal, strains of sub-genotype 2.2 were reported [80]. The situation in China is characterized by high variability of strains that belong mainly to sub-genotypes 1.1, 2.1, 2.2, and 2.3 [81,82,83,84]. Taiwan is also experiencing a change in sub-genotypes.…”

Section: Virus Propertiesmentioning

confidence: 99%

Classical Swine Fever—An Updated Review

Blome

Staubach

Henke

et al. 2017

Viruses

205

217

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Classical swine fever (CSF) remains one of the most important transboundary viral diseases of swine worldwide. The causative agent is CSF virus, a small, enveloped RNA virus of the genus Pestivirus. Based on partial sequences, three genotypes can be distinguished that do not, however, directly correlate with virulence. Depending on both virus and host factors, a wide range of clinical syndromes can be observed and thus, laboratory confirmation is mandatory. To this means, both direct and indirect methods are utilized with an increasing degree of commercialization. Both infections in domestic pigs and wild boar are of great relevance; and wild boars are a reservoir host transmitting the virus sporadically also to pig farms. Control strategies for epidemic outbreaks in free countries are mainly based on classical intervention measures; i.e., quarantine and strict culling of affected herds. In these countries, vaccination is only an emergency option. However, live vaccines are used for controlling the disease in endemically infected regions in Asia, Eastern Europe, the Americas, and some African countries. Here, we will provide a concise, updated review on virus properties, clinical signs and pathology, epidemiology, pathogenesis and immune responses, diagnosis and vaccination possibilities.

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Section: Virus Propertiesmentioning

confidence: 99%

Classical Swine Fever—An Updated Review

Blome

Staubach

Henke

et al. 2017

Viruses

205

217

View full text Add to dashboard Cite

show abstract

“…CSFV positive rate in clinical healthy pigs in GuanXi province of Southern China has been reported before, it's about 3% (Luo et al, 2011). The results indicated that C-strain vaccine can exist in pigs for a long period of time after vaccination, and persistently infected clinically healthy pigs exist in different breeding swine farms.…”

Section: Discussionmentioning

confidence: 61%

Development and Application of a RT-NestPCR Assay for Differential Detection of C-Strain and Wild-Type Viruses of Classical Swine Fever Virus

Wang¹,

Yi-bao²,

Cong³

et al. 2013

SAR

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Due to the urgent need of differentiation of infected from vaccinated animals in control and eradication of classical swine fever (CSF) and the shortcomings of current differential diagnostic tools, this study is aiming to establish a RT-nestPCR assay for differential detection of wild-type viruses and lapinized Chinese vaccine strain (C-strain) of classical swine fever virus (CSFV) of high sensitivity. Two pairs of CSFV-specific primers were designed in the conservative regions of NS5B (a non-structural protein encoded by the CSFV genome, which performs the RNA dependent RNA polymerase activity) and 3′ un-translated regions (3′-UTR) to encompass the T-rich insertion uniquely existing in the 3′-UTR of C-strain genome. Thus the amplification fragment of C-strain is longer than that of the wild-type viruses for it contains the T-rich insertion region. Two pairs of primers were used in combination and the wild-type viruses and C-strain of CSFV could be detected and accurately distinguished with a high sensitivity through super fine resolution (SFR) argarose gel electrophoresis that displays the different lengths of the amplicons. The detection limit of the C-strain and Shimen strain were respectively 4.5×10 -2 pg and 3.2×10 -2 pg of viral RNA. The results of the specificity test showed that this method can detect different strains of CSFV without amplifying other non-CSFV pathogens. The results of the detection of 400 clinical samples indicated that 16 samples were CSFV positive in total; in which 4 samples were C-Strain positive and 12 were wild-type CSFV positive. The total CSFV positive rate was 4%. The detection results of the 14 batches of C-Strain vaccines showed that all samples displayed bands of C-Strain amplicons in the SFR argarose gel electrophoresis and all vaccines were free of wild-type virus contamination. In conclusion, the RT-nestPCR assay established in the present study could supply a sensitive and specific test method for distinguishing wild-type CSFV infected animals from those vaccinated with C-strain vaccines in the field.

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“…This discrepancy may be due to the shortage of isolate data included in their analysis. Luo et al. (2011) reported that 11 of 31 CSFV isolates belonged to subgroup 1.1.…”

Section: Resultsmentioning

confidence: 99%

“…Groups 1 and 2 are composed of three subgroups (1.1–1.3 and 2.1–2.3) and group 3 of four subgroups (3.1–3.4). Molecular epidemiology studies of Asian CSFV isolates by Japanese (Sakoda et al., 1999), Thai (Parchariyanon et al., 2000), Vietnamese (Kamakawa et al., 2006), Korean (Cha et al., 2007), Chinese (Tu et al., 2001; Deng et al.,2005; Luo et al., 2011; Shen et al., 2011) and Indian (Patil et al., 2010, 2012) researchers have indicated all three genogroups are present in Asia, and within these genogroups, there is considerable diversity.…”

Section: Introductionmentioning

confidence: 99%

Genetic Typing of Classical Swine Fever Virus Isolates from China

Sun¹,

Yin²,

Guo

et al. 2012

Transboundary and Emerging Diseases

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The E2 genes of 73 classical swine fever virus (CSFV) originated from CSF suspected cases in different regions of China were genetically characterized and compared with reference CSF viruses. All Chinese viruses that characterized were segregated into two major groups and subdivided into four subgroups. Most of isolates (61.6%) belonged to group 2 and were further divided into three subgroups: subgroup 2.1, 2.2 and 2.3. Subgroup 2.1 was the largest subgroup which contained 46.6% of isolates, while subgroup 2.3 was the smallest subgroup which contained only one isolate (1.4%). The remaining 38.4% of isolates were classified into subgroup 1.1 within group 1. However, no group 3 and subgroups 1.2 and 1.3 viruses were found in this study. This study has provided epidemiological information useful for assessing the virus origin and establishing a national prevention and control strategy against the disease.

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Phylogenetic analysis of the E2 gene of classical swine fever virus from the Guangxi Province of southern China

Cited by 23 publications

References 24 publications

Classical Swine Fever—An Updated Review

Classical Swine Fever—An Updated Review

Development and Application of a RT-NestPCR Assay for Differential Detection of C-Strain and Wild-Type Viruses of Classical Swine Fever Virus

Genetic Typing of Classical Swine Fever Virus Isolates from China

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