Phylogenetic analysis of Suid Herpesvirus 1 isolates from Argentina

Serena, María Soledad; Metz, Germán Ernesto; Mórtola, Eduardo; Echeverria, M

doi:10.1016/j.vetmic.2011.06.028

Cited by 11 publications

(16 citation statements)

References 31 publications

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“…Among the numerous restriction endonucleases tested, BamH I has been most widely used for RFLP analysis, as shown in several reports mainly studying strains isolated from Europe, Japan, Argentina, and Brazil (Muller et al, 2010;Nishimori et al, 1987;Piatti et al, 2001;Serena et al, 2011). In a previous study, PRV isolates from European and American countries were divided into 4 major types and several subtypes by RFLP analysis, but Chinese strains were not included (Herrmann et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes

et al. 2015

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Recently pseudorabies outbreaks have occurred in many vaccinated farms in China. To identify genetic characteristics of pseudorabies virus (PRV) strains, we obtained the genomic sequences of PRV strains HeN1 and JS, which were compared to 4 PRV genomes and 729 partial gene sequences. PRV strains isolated in China showed marked sequence divergence compared to European and American strains. Phylogenetic analysis revealed that for the first time PRV can be divided into 2 distinct clusters, with Chinese strains being genotype II and PRVs isolated from other countries being genotype I. Restriction fragment length polymorphism analysis confirmed differences between HeN1 and Bartha strains, as did the presence of unique insertion/deletion polymorphisms and microsatellites. This divergence between the two genotypes may have been generated from long-term, independent evolution, which could also explain the low efficacy of the Bartha vaccine in protecting pigs infected with genotype II PRV.

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Section: Introductionmentioning

confidence: 99%

Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes

et al. 2015

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“…Type III and type IV PRV strains are restricted to Northern Europe and Asia [2]. Serena et al [28] Here it must be emphasized that there is a limited number of PPV nucleotide sequences available at GenBank. Xu et al [9] compared the nucleotide sequence of the VP2 gene of the PPV NE/09 strain and other PPV strains in China.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

et al. 2016

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The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identifi ed in this study showed high similarity and signifi cant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and signifi cant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identifi ed in swine in Republic of Montenegro. Besides that, these results confi rmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.

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“…We also found that CL-15 gE is more similar to the gE proteins from Western isolates than to Asian isolates of SHV-1. This suggests that gE proteins from Asian SHV-1 isolates are genetically distinct from gE proteins encoded by SHV-1 strains found in the Western world, which has not been noted in previous studies [27, 28]. SDS-PAGE analysis of AcgE-infected High Five cell lysates revealed the presence of a protein around 72 kDa, which was not present in lysates from control baculovirus-infected cells and was immunoreactive with monoclonal and polyclonal SHV-1 gE-specific antibodies.…”

Section: Discussionmentioning

confidence: 57%

Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs

Serena

Geisler

Metz

et al. 2013

Protein Expression and Purification

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Suid Herpesvirus 1 (SHV-1) is the etiological agent of Aujeszky’s disease (AD), which affects swine herds worldwide and causes substantial economic losses due to animal mortality and lost productivity. In order to eradicate SHV-1, vaccination programs using viruses lacking the gene encoding glycoprotein E (gE) are ongoing in several countries. These eradication programs have generated a currently unmet demand for affordable and sensitive tests that can detect SHV-1 infection, yet distinguish between infected and vaccinated pigs. To meet this demand, we used the baculovirus-insect cell system to produce immunologically authentic full-length recombinant gE protein for use in a serum ELISA assay. As previous efforts to clone the gE gene had failed due to its extremely high GC-content (75% average), we used betaine as a PCR enhancer to facilitate amplification of the entire gE gene from the Argentinian CL15 strain of SHV-1. The cloned gE gene was expressed at high levels in recombinant baculovirus-infected insect cells and reacted strongly with sera from SHV-1 infected pigs. We used the recombinant gE protein to develop a local indirect ELISA test with sensitivity and specificity comparable to currently available commercial tests. Thus, recombinant gE produced in baculovirus-infected insect cells is a viable source of antigen for the detection of SHV-1 in ELISA tests. We also provide evidence supporting a potential application of this recombinant form of gE as a SHV-1 subunit vaccine.

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Phylogenetic analysis of Suid Herpesvirus 1 isolates from Argentina

Cited by 11 publications

References 31 publications

Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes

Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs

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