Phylogenetic analysis of small-ruminant lentivirus subtype B1 in mixed flocks: Evidence for natural transmission from goats to sheep

Pisoni, G.; Quasso, Antonio; Moroni, P.

doi:10.1016/j.virol.2005.06.013

Cited by 87 publications

(57 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Whilst VMV and CAEV were originally considered to be specific to sheep and goats, respectively, most subtypes have been isolated from both host species (Gjerset et al, 2007;Leroux et al, 1997;Reina et al, 2006;Rolland et al, 2002;Shah et al, 2004a;Zanoni, 1998), suggesting that cross-species infection may occur under field conditions. This presumption has been confirmed by phylogenetic analyses of SRLV sequences isolated among sheep and goats from mixed flocks, providing direct evidence for natural interspecies transmission of subtype A4 in both directions (Shah et al, 2004b), as well as subtype B1 and group C from goats to sheep (Gjerset et al, 2007;Pisoni et al, 2005).…”

Section: Introductionmentioning

confidence: 87%

Field evaluation of a gag/env heteroduplex mobility assay for genetic subtyping of small-ruminant lentiviruses

Germain

Croisé

Valas

2008

Journal of General Virology

View full text Add to dashboard Cite

Small-ruminant lentiviruses (SRLVs) display a high genetic diversity and are currently classified into five genotypes and an increasing number of subtypes. The co-circulation of subtypes in restricted geographical regions, combined with the occurrence of cross-species infection, suggests the need for development of a large-scale screening methodology for rapid monitoring of the prevalence of the various genetic subtypes and their genetic evolution. Here, a heteroduplex mobility assay (HMA) was developed for the rapid identification of group B subtypes. The assay was validated for both the p14 nucleocapsid-coding region of the gag gene and the V1-V2 region of the env gene using a panel of reference standards and was applied to the genetic subtyping of SRLV field isolates from five mixed flocks in France. Subtyping of 75 blood samples using the env HMA revealed a preferential distribution of subtypes B1 and B2 in sheep and goats, despite direct evidence for interspecies transmission of both subtypes. Adding the gag HMA to the env HMA provided evidence for dual infection and putative recombination between subtypes B1 and B2 in five goats, and between groups A and B in one sheep. Phylogenetic analysis revealed that 100 % (23/23) and 96.7 % (30/31) of samples were correctly classified using the gag and env HMAs, respectively. These results indicate that dual infection and recombination may be a significant source of new variation in SRLV and provide a useful tool for the rapid genetic subtyping of SRLV isolates, which could be relevant for the development of more accurate diagnosis of prevalent SRLV strains in different countries.

show abstract

Section: Introductionmentioning

confidence: 87%

Field evaluation of a gag/env heteroduplex mobility assay for genetic subtyping of small-ruminant lentiviruses

Germain

Croisé

Valas

2008

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The CAE virus (CAEV) and Maedi-Visna virus (MVV) compose the group of small ruminant lentiviruses, which are antigenically related but genetically distinct (PISONI; QUASSO; MORONI, 2005;PISONI et al, 2007;ICTV, 2013). Sheep and goats reared together can be infected with both CAEV and MVV circulating in the herd.…”

Section: Introductionmentioning

confidence: 99%

“…This knowledge changed the approach of the epidemiological aspects of the disease as well as its control and eradication. As a result, the etiologic agent of CAE has been referred to as small ruminant lentivirus (SRLV) (BERTONI, 2007;QUASSO;MORONI, 2005;PISONI et al, 2007;GJERSET et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Use of serological diagnostic techniques in the control and eradication of caprine arthritis encephalitis: an update

Mussi

Gouveia

Cortéz

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

Caprine arthritis encephalitis (CAE) is a chronic disease caused by a small ruminant lentivirus (SRLV), which causes significant losses in goat breeding. The actual state of animal infection with SRLV is difficult to determine due to a complex pathogenesis of the virus, including factors such as delayed or intermittent seroconversion in serological tests. Several serological techniques are available for disease diagnosis, such as screening or confirmation tests, which are different in sensitivity and specificity. Regarding the choice of the test to be applied, availability of commercial immunoreagents, team training, antigen used, and cost of techniques must be considered. This review presents the serological methods available for use in different stages of CAE control and eradication programs, and management measures to be adopted in conjunction with serological diagnosis of the disease.Keywords: Caprine arthritis encephalitis virus. CAEV. Small ruminant lentivirus. SRLV. Diagnosis. Control. ResumoA artrite encefalite caprina (CAE) é uma enfermidade crônica causada por um lentivírus de pequenos ruminantes (LVPR), que ocasiona perdas significativas na caprinocultura. O estado real da infecção animal pelo LVPR é de difícil determinação em virtude da complexa patogenia do vírus, incluindo fatores como soroconversão tardia ou intermitente em testes sorológicos. Para o diagnóstico da enfermidade, diversas técnicas sorológicas estão disponíveis, como testes de triagem ou confirmatórios, com variações na sensibilidade e especificidade. Para escolha do teste a ser usado, a disponibilidade de imunorreagentes comerciais, o treinamento da equipe, o antígeno utilizado, e o custo das técnicas devem ser considerados. Esta revisão apresenta os métodos sorológicos disponíveis para uso em diferentes fases dos programas de controle e erradicação da CAE e as medidas de manejo que devem ser adotadas em conjunto com o diagnóstico sorológico da enfermidade. Palavras

show abstract

“…For many years following their isolation in 1960 for MVV and 1980 for CAEV they had been recognized as distinct pathogens, infecting two different ruminant species -sheep and goats, respectively (Sigurdsson et al 1960, Crawford et al 1980. Last years have brought the new view of their nature, revealing that they are capable of crossing the interspecies barrier not only in experimental but also natural conditions (Leroux et al 1997, Castro et al 1999, Shah et al 2004a, Pisoni et al 2005. Nowadays both these viruses are classed together as small ruminant lentiviruses (SRLV), which are further split into five groups from A to E (Shah et al 2004b, Grego et al 2007.…”

Section: Introductionmentioning

confidence: 99%

Influence of chronic caprine arthritis-encephalitis virus infection on the population of peripheral blood leukocytes

Kaba¹,

Winnicka²,

Zaleska³

et al. 2011

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The influence of caprine arthritis-encephalitis (CAE) virus infection on the population of peripheral blood leukocytes in goats was evaluated. For this purpose two groups of adult dairy female goats were formed. The experimental group consisted of 17 goats, which had been naturally infected for many years. The control group comprised 29 non-infected goats, which originated from CAE-free herd. All goats were clinically healthy. Whole blood was collected and tested in hematological analyzer and light microscope to assess the total number of leukocytes and the percentage of four leukocyte populations -neutrophils, eosinophils, monocytes and lymphocytes. Then, flow cytometry with monoclonal antibodies against several surface antigens (namely CD14, CD2, B-B2, CD4, CD8h, TCR-N6, WC1-N2 and WC1-N3) was performed to assess the proportion of lymphocyte subpopulations. Statistically significant differences (α ≤ 0.01) were observed only in the subpopulations of T lymphocytes -percentage of all subpopulations were significantly higher in the group of seropositive goats. No statistically significant differences were revealed with respect to the total number of blood leukocytes, the average percentage of blood leukocyte populations and proportions of both T and B lymphocytes.

show abstract

Phylogenetic analysis of small-ruminant lentivirus subtype B1 in mixed flocks: Evidence for natural transmission from goats to sheep

Cited by 87 publications

References 26 publications

Field evaluation of a gag/env heteroduplex mobility assay for genetic subtyping of small-ruminant lentiviruses

Field evaluation of a gag/env heteroduplex mobility assay for genetic subtyping of small-ruminant lentiviruses

Use of serological diagnostic techniques in the control and eradication of caprine arthritis encephalitis: an update

Influence of chronic caprine arthritis-encephalitis virus infection on the population of peripheral blood leukocytes

Contact Info

Product

Resources

About