Phylogenetic analysis of porcine parvoviruses from swine samples in China

Hao, Xiao-Fang; Lu, Zengjun; Sun, Pu; Fu, Yuanfang; Cao, Yimei; Li, Pinghua; Bai, Xingwen; Bao, Huang; Xie, Baoxia; Chen, Yingli; Li, Dong; Liu, Zaixin

doi:10.1186/1743-422x-8-320

Cited by 21 publications

(19 citation statements)

References 17 publications

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“…The family Parvoviridae is very diverse and the subfamily Parvovirinae contains several genera known to infect pigs including the genus Bocavirus (Hao et al, 2011;Zhang et al, 2011a,b). In 2009, a novel porcine boca-like virus (Pbo-likeV) was identified in lymph nodes obtained from two Swedish pigs suffering from systemic PCVAD using random multiple displacement amplification and large-scale sequencing (Blomström et al, 2009).…”

Section: Porcine Parvovirusmentioning

confidence: 99%

Concurrent infections are important for expression of porcine circovirus associated disease

2012

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Porcine circovirus type 2 (PCV2) is the essential component of porcine circovirus disease (PCVD) as the disease syndrome is referred to in Europe and porcine circovirus associated disease (PCVAD) as it is referred to in North America. Singular PCV2 infection rarely results in clinical disease; however, PCVAD is often accelerated in onset, enhanced in severity and prolonged in duration by concurrent viral or bacterial infections. Due to its effect on the immune system, PCV2 has also been shown to enhance protozoal, metazoal, and fungal infections. Several retrospective or cross-sectional studies have investigated the presence and prevalence of various infectious agents associated with PCVAD under field conditions. Experimental models confirm that PCV2 replication and associated lesions can be enhanced by concurrent infection with other viruses or bacteria. The exact mechanisms by which concurrent pathogens upregulate PCV2 are unknown. Co-infections may promote PCV2 infection by increasing immune host cell replication and accumulation in tissues thereby enhancing targets for PCV2 replication. It has also been proposed that co-infections interfere with PCV2 clearance by alteration of cytokine production and profiles. The outcome of differences in timing of co-infections in PCV2-infected pigs is also likely very important and is an area where more research is needed. Given the current knowledge base, it is important that veterinarians do a thorough diagnostic investigation on herds where PCVAD is a recurrent problem in order to implement the most appropriate and cost effective intervention strategies.

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Section: Porcine Parvovirusmentioning

confidence: 99%

Concurrent infections are important for expression of porcine circovirus associated disease

2012

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“…VP2, the main nucleocapsid protein component of PPV, has hemagglutination activity and can induce neutralizing antibodies. Previous studies have shown that the major antigenic site regions of VP2 are amino acids 60-68, 81-88, 266-275, 351-357, and 398-404, where amino acids 378, 383, and 436 are critical sites to determine PPV virulence [15][16][17][18].…”

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Two novel protein chips for the detection of antibodies against porcine parvovirus

Hou³

et al. 2020

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Background : PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), and the modified direct complement-fixation (MDCF) test were utilized earlier, whereas the enzyme-linked immunosorbent assay (ELISA) is the most frequently applied assay to detect PPV-specific antibodies Results: We establish the visible protein chip and the cyanine dye 3 (Cy3)-labeled protein chip to detect the clinical serum from pigs. In this study, the recombinant protein VP2 of PPV was expressed in E.coli , purified with nickel magnetic beads, and then printed onto epoxy-coated glass slides for preparation of the protein chip. After a series of experiments, the conditions of antigen protein concentration, incubation time of primary antibody or secondary antibody, and optimal serum dilution fold were optimized, resulting in a successful visible protein chip and Cy3-labeled protein chip. The results showed that the positive serum, diluted up to 6000-fold, can be detected by the visible protein chip, and the positive serum, diluted up to 12,800-fold, can be detected by the Cy3-labeled protein chip, suggesting the high sensitivity of these protein chips. Moreover, the positive detection ratio, sensitivity, and specificity of these two kinds of protein chips were higher than those of commercial ELISA antibody detection kits. Conclusion: Overall, these two protein chips can be used to rapidly diagnose clinical samples with high throughput. Key words: Protein chip; Porcine parvovirus (PPV); Antibody detection; Clinical serum

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“…Detection of the virus in pigs is usually carried out by employing the polymerase chain reaction (PCR) and several PCR protocols for detection of the virus have been reported [11,12]. Phylogenetic analysis of PPV is commonly carried out on the basis of VP2 and NS1 gene sequences [3,9].…”

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confidence: 99%

“…Phylogenetic analysis of porcine parvoviruses based on nucleotide sequence of the VP2 and NS1 has been carried out by many researchers for molecular epidemiological studies [3,9]. Apart from data obtained from conventional sequencing, researchers are now resorting to metagenomics data to obtain sequences of novel parvoviruses in the pig population [8].…”

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First report of detection and molecular characterization of porcine parvovirus in domestic and wild pigs in Kerala, India

et al. 2016

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Porcine parvovirus (PPV) is a DNA virus of the genus Parvovirus of family Parvoviridae. It is the causative agent of many disease problems in pigs such as maternal reproductive failure, stillbirth, mummification, embryonic or fetal death, infertility, abortion and neonatal death. A study was conducted to assess the incidence of the virus in pigs in Kerala State in South India. A total of 38 samples were collected from domestic and wild pigs from different districts of the State. Polymerase chain reaction targeting a 265 bp fragment of the NS1 gene of the virus was carried out. Of the samples tested, 2 (5.26 %) were found to be positive for PPV virus genome, one of which was from a wild pig. One of the positive samples was sequenced and the nucleotide sequence obtained was compared with other sequences of PPV from India and abroad. The results revealed that the sequence had very close similarity to PPV sequences previously reported from India and to that of Chinese isolates. This is the first report of the existence of PPV in domestic and wild pigs in Kerala, India. The study highlights the need to test for the presence of PPV in addition to other infectious agents in diagnosis of cases of reproductive disorders in pigs.

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Phylogenetic analysis of porcine parvoviruses from swine samples in China

Cited by 21 publications

References 17 publications

Concurrent infections are important for expression of porcine circovirus associated disease

Concurrent infections are important for expression of porcine circovirus associated disease

Two novel protein chips for the detection of antibodies against porcine parvovirus

First report of detection and molecular characterization of porcine parvovirus in domestic and wild pigs in Kerala, India

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