Phylogenetic analysis of pestiviruses from domestic and wild ruminants.

Becher, Paul; Orlich, Michaela; Shannon, Anthony; Horner, G.W.; König, Matthias; Thiel, Heinz-Jürgen

doi:10.1099/0022-1317-78-6-1357

Cited by 279 publications

(187 citation statements)

References 43 publications

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“…2,26,32,33 Some reports suggest that the 59-UTR region might not serve as an appropriate target sequence for phylogenetic analysis because of its highly conserved nature. 3 However, several investigators demonstrated that the 59-UTR region is suitable for subtyping BVDV isolates. 25,26,32 The 59-UTR region is efficiently amplified by RT-PCR and is the most frequently analyzed portion of the genome.…”

Section: Resultsmentioning

confidence: 99%

Characterization and Phylogenetic Analysis of Bovine Viral Diarrhea Virus in Brain Tissues from Nonambulatory (Downer) Cattle in Korea

et al. 2010

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Abstract. Between August 2008 and May 2009, 386 brain and serum samples from adult cattle (2-7 years old) showing a variety of clinical signs of downer cow syndrome were received by the National Veterinary Research and Quarantine Service. All brain samples were tested for the presence of Bovine viral diarrhea virus (BVDV) by reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry (IHC), and antigen capture ELISA (Ag-ELISA). The BVDV nucleic acid was detected in 54 of 386 (15.5%) brain samples tested by RT-PCR. Positive results were detected in 14 (3.67%) and 13 (3.4%) of samples tested by IHC and Ag-ELISA, respectively. Both BVDV nucleic acid and antigen were detected in 11 cattle (2.9%) by all 3 diagnostic tests; however, antibodies against BVDV were not detected in these 11 cattle. A molecular classification of the identified viral strains (n 5 40) was also carried out. Neighbor-joining phylogenetic analysis revealed that most of the identified viruses belonged to BVDV genotype 1a (n 5 10), 1b (n 5 16), and 2a (n 5 8). The remaining strains were subtypes 1c (n 5 1), 1n (n 5 4), and 1m (n 5 1). Interestingly, most of the BVDV-1b strains (n 5 9) identified in brain samples were confirmed by all 3 diagnostic tests. Further studies should be performed to determine why the BVDV-1b strain was found in brain samples that were positive using all 3 diagnostic tests.

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Section: Resultsmentioning

confidence: 99%

Characterization and Phylogenetic Analysis of Bovine Viral Diarrhea Virus in Brain Tissues from Nonambulatory (Downer) Cattle in Korea

et al. 2010

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“…Later, hybridization and more extensive sequence analysis revealed that the 5′NCR was highly conserved in BVDV genomes. This led to the suggestion that the 5′NCR region might not serve as a good target sequence for phylogenetic studies because of its highly conserved nature [5]. However, numerous investigators demonstrated that the 5′ NCR region provides a useful tool in genotyping BVDV isolates [6,32,22,26].…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of bovine viral diarrhoea (BVD) viruses: confirmation of the presence of BVD genotype 2 in Africa

et al. 2012

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Bovine viral diarrhoea virus (BVDV) has emerged as one of the economically important pathogens in cattle populations with a worldwide distribution causing a complex of disease syndromes. Two genotypes BVDV 1 and 2 exist and are discriminated on the basis of the sequence of the 5′ non-coding region (5 NCR) using real-time PCR. The use of the real-time PCR is more sensitive, specific, and less time consuming than a conventional PCR, and has reduced the risk of cross-contamination of samples. Limited information exists on BVDV genetic subtypes in South Africa. The aim of this study was to determine the genotypes of

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“…Early antigenic analysis revealed that ovine pestiviruses can be divided into two groups which include BDV-like and BVDVlike strains [11]. Recently, it has been demonstrated that ovine pestiviruses are divided into BDV, BVDV1 and BVDV2, both at the antigenic and genetic level [3,15,23,26]. The difficulty of distinguishing between the pestivirus genotypes and their ability to cross host-species barriers emphasise the value of laboratory typing of pestivirus strains.…”

Section: Introductionmentioning

confidence: 99%

A RT-PCR assay for the rapid recognition of border disease virus

Vilček

Paton²

2000

Vet. Res.

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-A reverse transcription -polymerase chain reaction (RT-PCR) method was developed for the specific detection of border disease virus (BDV), using the primers PBD1 and PBD2 flanking a 225 bp DNA fragment, selected from the 5´noncoding region of the pestivirus genome. In tests on 70 pestiviruses it was shown to be BDV-specific. A closed, one-tube nested RT-PCR method employing general pestivirus outer primers (324 and 326), and the same BDV-specific inner primers (PBD1 and PBD2) in conjunction with a BDV-specific fluorogenic TaqMan probe also detected only BDV and was more sensitive. BDV-specific RT-PCR was used in combination with a PCR specific for bovine viral diarrhoea virus type 2 (BVDV2) to ascertain whether virus stocks contained mixtures of BDV and BVDV2. It was shown that the ovine pestivirus strains 175375 and 59386 were originally BDV, but after subculture had become contaminated with BVDV2. This explains a previously reported discrepancy in the genetic typing of 59386. Although the BDV-specific RT-PCR can also detect BDV in clinical samples, the assay is likely to be most useful for the rapid typing of laboratory pestivirus strains. border disease virus / BDV / RT-PCR / typing / pestivirus contaminationRésumé -Identification rapide du border disease virus par RT-PCR. Une méthode d'amplification en chaîne par polymérase après transcription inverse (RT-PCR) a été développée pour la détection spé-cifique du border disease virus (BDV), utilisant les amorces PBD1 et PBD2 flanquant un fragment d'ADN de 225 pb, choisi dans la région 5' non codante du génome des pestivirus. Des tests sur 70 souches de pestivirus ont démontré la spécificité de la méthode. Une méthode de RT-PCR nichée dans un seul tube fermé a également permis de détecter spécifiquement le BDV, tout en améliorant la sensibilité. Cette dernière méthode utilise des amorces externes générales pour les pestivirus (324 et 326), les amorces internes PBD1 et PBD2 ainsi qu'une sonde fluorogénique TaqMan reconnaissant spécifiquement le BDV. Les stocks de virus ont été testés à la fois par RT-PCR spécifique du BDV et RT-PCR spécifique du virus de la diarrhée bovine type 2 (BVDV2), afin de déterminer s'ils contenaient un mélange des deux virus. Il a ainsi été montré que les souches de pestivirus ovins 175375 Vet. Res. 31 (2000)

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Phylogenetic analysis of pestiviruses from domestic and wild ruminants.

Cited by 279 publications

References 43 publications

Characterization and Phylogenetic Analysis of Bovine Viral Diarrhea Virus in Brain Tissues from Nonambulatory (Downer) Cattle in Korea

Characterization and Phylogenetic Analysis of Bovine Viral Diarrhea Virus in Brain Tissues from Nonambulatory (Downer) Cattle in Korea

Genetic characterization of bovine viral diarrhoea (BVD) viruses: confirmation of the presence of BVD genotype 2 in Africa

A RT-PCR assay for the rapid recognition of border disease virus

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