Phylogenetic analysis of lumpy skin disease virus isolates in Russia in 2019-2021

Krotova, Alena; Mazloum, Ali; Byadovskaya, Olga; Sprygin, Alexander

doi:10.1007/s00705-022-05487-7

Cited by 9 publications

(6 citation statements)

References 23 publications

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“…Moreover, the large size of Tabanidae fl ies points that their fl ight patterns can cover a bigger distance, further increasing the chances that they can travel and transmit LSDV from one herd of animals to another within 1-2 km [31]. Since the identity of insect-derived LSDV sequences aligned with those previously reported for the regions of study [3,32], it can be concluded that the positive insects were carrying, possibly spreading, the virus actively circulating in the region, taking into account that since 2017 no two same recombinant strains have been detected within one region [33]. On the other hand, segregation of animals effectively prevents the mechanical transmission of pathogens by tabanids [34].…”

Section: Discussionmentioning

confidence: 55%

Evidence of Lumpy Skin Virus DNA in Blood-Feeding Flies During Outbreaks in Russia in 2018-2019

et al. 2022

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In this study we report the testing of blood-feeding and synanthropic flies captured near animals affected by lumpy skin disease virus (LSDV) in Russia during the outbreaks in the Kurgan region in 2018 and Saratov region in 2019. The insects of interest were the stable fly Stomoxys calcitrans, Tabanidae horse flies, Culicoides midges and the house fly Musca domestica examined as individuals or pools. The obtained findings demonstrate that viral DNA was found in pools of S.calcitrans and M. domestica and in the head and abdomen of stable flies. This is the first report of LSDV DNA detection in Tabanidae flies from the field. The presented data are envisaged to help further guide the search for putative vectors of LSDV in different climatic regions and interpret laboratory-controlled experiments on vector-borne transmission of LSDV.

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Section: Discussionmentioning

confidence: 55%

Evidence of Lumpy Skin Virus DNA in Blood-Feeding Flies During Outbreaks in Russia in 2018-2019

et al. 2022

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“…Taken together, genetic characterization of the first LSD outbreak in Mongolia showed that the circulating strain belongs to the dominant lineage found in the region of South East Asia (Krotova, Byadovskaya, et al., 2022; Krotova, Mazloum, et al., 2022).…”

Section: Discussionmentioning

confidence: 93%

Genetic characterization and epidemiological analysis of the first lumpy skin disease virus outbreak in Mongolia, 2021

Sprygin

Sainnokhoi

Gombo-Ochir

et al. 2022

Transbounding Emerging Dis

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Novel lumpy skin disease virus (LSDV) strains of recombinant origin are on the rise in South East Asia following the first emergence in 2017, and published evidence demonstrates that such genetic lineages currently dominate the circulation. Mongolia reported first LSD outbreaks in 2021 in a north-eastern region sharing borders with Russia and China. For each of 59 reported LSDV outbreaks, the number of susceptible animals ranged from 8 to 8600 with a median of 572, while the number of infected animals ranged from one to 355 with a median of 14. Phylogenetic inferences revealed a close relationship of LSDV Mongolia/2021 with recombinant vaccine-like LSDV strains from Russia, China, Taiwan, Thailand and Vietnam. These findings support the published data that the circulating strain of LSDV belongs to the dominant recombinant lineage recently established in the region.

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“…However, it is clear that in order to provide a comprehensive insight into the LSDV proteome, that the same proteomic characterization deserves to be implemented on the other LSDV strains, with the newly emerged ones being of utmost interest. In particular, deciphering the proteomic profile of recombinant LSDV strains, recently evidenced for instance in Russia ( 99 , 100 ) could help in better understanding the mosaic nature of their genome, including regions from both vaccine and virulent field LSDV strains. Such larger proteomic characterization of CaPV strains may be especially enlightening, in particular for the comparison between virulent and attenuated viral strains, for which we could get possible clues upon the determinants of CaPV virulence.…”

Section: Discussionmentioning

confidence: 99%

Constitutive proteins of lumpy skin disease virion assessed by next-generation proteomics

Schlosser-Perrin,

Holzmuller,

Fernandez

et al. 2023

J Virol

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Lumpy skin disease (LSD) virus, a ruminant poxvirus of the Capripoxvirus genus, is the etiologic agent of an economically important cattle disease categorized as a notifiable disease by the World Organisation for Animal Health. The large and complex enveloped LSD virus (LSDV) particle encloses a double-stranded linear DNA genome of 151 kbp, comprising 156 predicted ORFs, together with a variety of proteins that are not yet identified. In parallel with a recent widespread expansion of LSD, many LSDV whole genomes have been sequenced, but knowledge on protein composition of the LSDV particle remains missing. In this study, LSD mature virions (MV) from strain KSGP-0240 were purified through a multistep ultracentrifugation process. The protein composition of LSD virions was then analyzed using label-free shotgun proteomics, based upon nano-liquid chromatography (LC) and tandem mass spectrometry. This procedure resulted in the identification of a total of 111 LSDV proteins. Considering that this first MV proteome extended beyond packaged proteins into the field of contaminants, an analytical methodology was developed and made it possible to select 66 viral proteins as candidates for packaging into MV. These viral proteins were analyzed comparatively with proteins previously demonstrated to be constitutive of the vaccinia virus MV particle. Offering for the first time a comprehensive proteomic analysis of an LSDV strain, this study contributes to our understanding of the structural features of infectious LSDV MV particles and paves the way for further systematic proteomic characterization of other LSDV strains. IMPORTANCE Lumpy skin disease virus (LSDV) is the causative agent of an economically important cattle disease which is notifiable to the World Organisation for Animal Health. Over the past decades, the disease has spread at an alarming rate throughout the African continent, the Middle East, Eastern Europe, the Russian Federation, and many Asian countries. While multiple LDSV whole genomes have made further genetic comparative analyses possible, knowledge on the protein composition of the LSDV particle remains lacking. This study provides for the first time a comprehensive proteomic analysis of an infectious LSDV particle, prompting new efforts toward further proteomic LSDV strain characterization. Furthermore, this first incursion within the capripoxvirus proteome represents one of very few proteomic studies beyond the sole Orthopoxvirus genus, for which most of the proteomics studies have been performed. Providing new information about other chordopoxviruses may contribute to shedding new light on protein composition within the Poxviridae family.

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Phylogenetic analysis of lumpy skin disease virus isolates in Russia in 2019-2021

Cited by 9 publications

References 23 publications

Evidence of Lumpy Skin Virus DNA in Blood-Feeding Flies During Outbreaks in Russia in 2018-2019

Evidence of Lumpy Skin Virus DNA in Blood-Feeding Flies During Outbreaks in Russia in 2018-2019

Genetic characterization and epidemiological analysis of the first lumpy skin disease virus outbreak in Mongolia, 2021

Constitutive proteins of lumpy skin disease virion assessed by next-generation proteomics

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