Phylogenetic Analysis of Korean Native Aster Plants Based on Internal Transcribed Spacer (ITS) Sequences

Abstract

This study was carried out to decide ITS (internal transcribed spacer) sequence of some Korean native Aster species and to resolve their relationship among Korean native Aster, including Kalimeris, Gymnaster, Heteropappus genus separated from Aster in a previously study based on the pappus length. We registered 11 ITS sequences of Aster species including A. glehni to GenBank and those sequences were used for the cluster analysis with Kalimeris species. The size of ITS1 was varied from 248 to 256 bp, while ITS2 was varied from 220 to 222 bp. The G + C content of the ITS region ranged from 49.4 to 53.5%. Pairwise comparison results showed that the substitution rate of ITS1 and ITS2 region was 9% and 10%, respectively. Kalimeris sensu strict substitution rate was lower than that of Aster sensu strict species. The strict consensus parsimonious cluster analysis showed A. tripolium is the first branching from the clade and the next is A. scaber. The Kalimeris species except for the A. hispidus were grouped into the same clade with high bootstrap value (91%) within Aster. Gymnaster and Heteropappus that has been classified by morphological characters were also grouped into broad sense Aster clade. These results implied these three genera could be merged together into Aster based on the ITS sequences.

Molecular Identification of Zoysia japonica and Zoysia sinica (Zoysia Species) Based on ITS Sequence Analyses and CAPS

“…DNA 바코드는 다양한 생물종에 공통적으로 보존 및 변이되어 존재하는 특정한 유전자 및 염기서열 구간이며, 생물의 아주 작은 조각에서도 DNA 분석이 가능하고 간단한 PCR 증폭과 염기서열 분석 을 통하여 미동정 생물체 동정에 활용되고 있다 (Hebert et al, 2003;Kress et al, 2005;Koch, 2010;Kim et al, 2015). 이러한 DNA 바코드 분석법은 생물다양성협약(Convention on Biological Diversity, CBD)의 국제적 생물종 분류기준(Consortium for the Barcode of Life, CBOL, http://barcoding.si.edu)으로 채택되어 나라별 고유종 및 신종 등록과 수집한 생물의 표본관리, 의약품 등 신물질 재료 판별 검증 등에 응용이 가능하여 식약처 및 환경부, 농림부 등 다양한 기관에서 생물 분류시 연구 및 활 용되고 있다 (Baigalmaa et al, 2009;Ahn et al, 2010;Hong et al, 2012;Moon et al, 2013). 생물 분류를 위한 DNA 바코드 분 석은 핵 내 ribosomal DNA(nrDNA)의 internal transcribed spacer (ITS) 염기서열 구간 등을 이용하여 연구되어 왔다 (Kress et al, 2005;Baigalmaa et al, 2009;Ahn et al, 2010;China Plant BOL Group et al, 2011;Hong et al, 2012;Kress and Erickson, 2008;Moon et al, 2013).…”

“…Additional key words: cleaved amplified polymorphic sequence, internal transcribed spacer, restriction endonuclease, species identification, Zoysia japonica, Zoysia sinica 양친 유전성을 가지며, 생물 종에 따라 보존 지역과 변이 지역이 뚜렷하여 polymerase chain reaction(PCR)을 통한 분리 및 염 기서열 분석에 유리하다 (Kress et al, 2005;CBOL Plant Working Group, 2009;Ahn et al, 2010;Hong et al, 2012). 또한, ITS 는 종간 및 속간 유연관계, 진화양상, 시중에 유통되는 한약제 위품들의 판별 등을 분석하는데 중요한 자료로도 이용되고 있다 (Baigalmaa et al, 2009;Gao et al, 2010;Yao et al 2010).…”

“…또한 들잔디와 갯잔디 두 종간의 전체 ITS 구간 염기서열들을 비교 분석한 결과, 치환된 염기의 수는 18개이며, 오직 1bp 의 염기만이 INDEL에 의한 차이임을 확인하였다 (Fig. 2) Kress et al, 2005;CBOL Plant Working Group, 2009;Ahn et al, 2010;Koch et al, 2010;Hong et al, 2012). 333bp, 200bp, 108bp와 306bp, 200bp, 133bp의 세 개의 밴드를, 갯잔디(Z)는 480bp와 91bp 두 개의 밴드 양 상을 확인하였다 (Fig.…”

“…According to differences in their ITS sequences, control and collected samples of Z. japonica and Z. sinica were classified into two Z. japonica groups (Zj-I and Zj-II), and four Z. sinica groups (Zs-I-Zs-IV); The number of Zj-I, Zj-II and Zs-I-IV groups are shown in Table 4. et al, 2005;Han et al, 2006;Kress and Erickson, 2008;Baigalmaa et al, 2009;Ahn et al, 2010;Gao et al, 2010;Yao et al 2010;Hong et al, 2012;Moon et al, 2013 Table 4. Table 4.…”

Phylogenetic relationships among Egyptian species of Anthemideae (Asteraceae) using ITS markers

“…According to Täckholm (1974), Anthemideae is represented in Egypt by 11 genera and 31 species. Nowadays, the molecular markers have been shown to be a powerful tool for assessing genetic diversity and genetic variation with polymorphism at the DNA level (Hong et al 2012). Molecular polymorphism derived from variation in the nuclear ribosomal DNA of the internal transcribed spacer (ITS), chloroplast DNA (trnL-trnF, atpB-rbcL, rpoC1, trnH-psbA), inter generic spacer (IGS), external transcribed spacer (ETS), and simple sequence repeat (SSR) technology have been used in plant classification (Ahn et al 2010;Felsenstain 1985;Masuda et al 2009).…”