Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences

Noël, Christophe; Peyronnet, Corinne; Gerbod, Delphine; Edgcomb, Virginia P.; Delgado‐Viscogliosi, Pilar; Sogin, Mitchell L.; Capron, Moníque; Viscogliosi, Éric; Zenner, Lionel

doi:10.1016/s0166-6851(02)00246-3

Cited by 85 publications

(50 citation statements)

References 26 publications

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“…The rooted UPGMA tree identified 7 clades, which corresponded to the 7 known groups/ subtypes identified in previous molecular studies (Arisue et al 2003 ;Abe et al 2003 a, b;Noel et al 2003Noel et al , 2005. There was strong bootstrap support for the clustering of subtypes 2 and 4 to the exclusion of subtypes 1, 3, 5, 6 and 7, but only moderate support for the delineation of subtypes 1, 5 and 6 from subtypes 3 and 7, as well as subtype 2 from 4 (see Fig.…”

Section: Phylogenetic Analysismentioning

confidence: 66%

“…Previous molecular characterization studies of Blastocystis have only analysed Blastocystis isolates amplified in vitro (Ho et al 2000 ;Arisue et al 2003 ;Noel et al 2003Noel et al , 2005Yoshikawa et al 2004 c). The present study was the first to compare isolates obtained from in vitro cultures and those amplified directly from the faeces by the PCR.…”

Section: Preferential Growth Of Blastocystis In Vitromentioning

confidence: 99%

“…Recent molecular studies have shown that Blastocystis displays considerable genetic heterogeneity in conserved genes, such as the small subunit of nuclear ribosomal RNA gene (SSU) and the elongation factor-1a gene (Ho et al 2000 ;Arisue et al 2003 ;Yoshikawa et al 2003Abe et al 2003 a, b;Noel et al 2003Noel et al , 2005Abe, 2004). Studies based on the SSU rRNA gene have revealed the presence of 7 groups or subtypes of Blastocystis displaying low host specificity.…”

Section: Introductionmentioning

confidence: 99%

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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

et al. 2006

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In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.

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Section: Phylogenetic Analysismentioning

confidence: 66%

Section: Preferential Growth Of Blastocystis In Vitromentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

et al. 2006

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“…[1][2][3][4][5][6] Individuals infected with Blastocystis were mostly asymptomatic, whereas some patients presented with acute or chronic gastrointestinal illness. [7][8][9] Blastocystis infection has been reported from both developed and developing countries.…”

Section: Introductionmentioning

confidence: 99%

Identification of Blastocystis Subtype 1 Variants in the Home for Girls, Bangkok, Thailand

Thathaisong¹,

Siripattanapipong²,

Mungthin³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract.A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocystis was performed by polymerase chain reaction and sequence analysis of the partial small subunit ribosomal RNA (SSU rRNA) gene. Subtype 1 was the most predominant (94.8%), followed by subtype 6 (3.5%) and subtype 2 (1.7%). Sequence analyses revealed nucleotide polymorphisms for Blastocystis subtype 1, which were described as subtype 1/variant 1, subtype 1/variant 2. Blastocystis subtype 1/variant 1 was the most predominant infection occurring in almost every house. The results showed that subtype analysis of Blastocystis was useful for molecular epidemiological study.

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“…Currently, human, mammalian, avian, and reptilian isolates can be assigned to one of 10 subtypes. [12][13][14][15][16] However, it is still not clear whether any of these subtypes are specific to human disease. Most human Blastocystis infections were shown to be attributed to subtype 3, having been reported in 40-92% of fecal samples.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples

Roberts¹,

Barratt²,

Harkness³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

110

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Abstract. We tested 513 stool samples from patients in Sydney, Australia for Blastocystis by using five diagnostic techniques: microscopy of a permanently stained smear using a modified iron-hematoxylin stain, two xenic culture systems (a modified Boeck and Drbohlav's medium and tryptone, yeast extract, glucose, methionine-9 medium), and two published conventional polymerase chain reaction methods specific for the small subunit ribosomal DNA. Ninety-eight (19%) samples were positive for Blastocystis in one or more of the diagnostic techniques. The PCR 2 method was the most sensitive at detecting Blastocystis with a sensitivity of 94%, and the least sensitive was microscopy of the permanent stain (48%). Subtype 3 was the most predominant subtype (present in 43% of samples assigned to this group). This study highlights the low sensitivity of microscopy when used as the sole diagnostic modality for detection of Blastocystis sp.

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Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences

Cited by 85 publications

References 26 publications

Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

Identification of Blastocystis Subtype 1 Variants in the Home for Girls, Bangkok, Thailand

Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples

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