Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

Parkar, Unaiza; Traub, Rebecca J.; Kumar, Suresh; Mungthin, Mathirut; Vitali, Simone; Leelayoova, Saowanee; Morris, Keith; Thompson, R.C.A.

doi:10.1017/s0031182006001582

Cited by 164 publications

(169 citation statements)

References 44 publications

(61 reference statements)

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“…The results from this study, with PCR being the most effective form of diagnosis, are consistent with those of studies that reported that molecular analysis is the most efficient method for detection of Blastocystis . 12,23 This finding is in contrast to those of another study, 20 which suggested that in vitro culture was superior to direct PCR for stool samples.…”

Section: Discussioncontrasting

confidence: 76%

See 1 more Smart Citation

Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples

Roberts¹,

Barratt²,

Harkness³

et al. 2011

The American Society of Tropical Medicine and Hygiene

112

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Abstract. We tested 513 stool samples from patients in Sydney, Australia for Blastocystis by using five diagnostic techniques: microscopy of a permanently stained smear using a modified iron-hematoxylin stain, two xenic culture systems (a modified Boeck and Drbohlav's medium and tryptone, yeast extract, glucose, methionine-9 medium), and two published conventional polymerase chain reaction methods specific for the small subunit ribosomal DNA. Ninety-eight (19%) samples were positive for Blastocystis in one or more of the diagnostic techniques. The PCR 2 method was the most sensitive at detecting Blastocystis with a sensitivity of 94%, and the least sensitive was microscopy of the permanent stain (48%). Subtype 3 was the most predominant subtype (present in 43% of samples assigned to this group). This study highlights the low sensitivity of microscopy when used as the sole diagnostic modality for detection of Blastocystis sp.

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Section: Discussioncontrasting

confidence: 76%

“…[10][11][12]23 In our study, two media, MBD and TYGM-9, were used for comparison of growth of Blastocystis in culture. These culture media were used instead of Jones medium because it was known that Blastocystis grows successfully in Jones medium.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples

Roberts¹,

Barratt²,

Harkness³

et al. 2011

The American Society of Tropical Medicine and Hygiene

112

View full text Add to dashboard Cite

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“…The main rout of the transmission in humans is fecaloral, but molecular studies revealed that a zoonotic transmission can occur (11). Based on studies conducted on a genetic diversity using the small subunit rDNA (SSU-rDNA), at least 17 subtypes (STs) of Blastocystis were identified in humans and animals (2).…”

Section: Introductionmentioning

confidence: 99%

Comparative Prevalence of Blastocystis in Patients with the Irritable Bowel Syndrome and Healthy Individuals: A Case Control Study

Beiromvand

Hashemi

Arjmand

et al. 2017

Jundishapur J Microbiol

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Background: Blastocystis is one of the most common anaerobic protozoa found in the intestinal tract of humans and various animals, with a worldwide distribution. The parasite has been linked to the pathogenesis of the irritable bowel syndrome (IBS), previously. Objectives: The aim of this study was to evaluate the prevalence of Blastocystis in IBS patients compared to healthy individuals. Methods: The collected feces from 152 patients with Gastrointestinal (GI) symptoms, and 130 healthy volunteers from Ahvaz, southwest Iran, were examined using the direct saline smear, Lugol's iodine staining, and inoculated in a Jones' medium for Blastocystis detection. The DNA was extracted from all culture-positive samples, and then the polymerase chain reaction (PCR) was performed by the SSU-rDNA gene. Results: Blastocystis was identified in 18 (6.4%) samples, including two (1.3%) of the IBS patients and 16 (12.3%) of the control group by microscopy. Stool culture was positive in 15 with IBS, one without IBS, and 40 control samples. From these, the expected 600 bp fragments of the SSU-rDNA gene were identified in 15 (27.3%) cases and 40 (72.7%) controls. Subtypes (STs) 1, 2, and 3 were identified from the 54 successfully sequenced samples. Subtype 3 was the most common ST with the frequency of 46.3%, followed by ST2, 37% and ST1, 16.7% in the case and control groups. The highest frequency of Blastocystis STs (27.8%) was identified in the age group of 31-40 years and the lowest was found in the age groups of under 10 years and over 81 years. Conclusions:The findings of the current study showed that Blastocystis was more common in the control group compared to the IBS patients. Therefore, our findings highlight the contrast between Blastocystis infection and GI disorders. Furthermore, these results support the hypothesis that Blastocystis could be a GI health marker.

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“…So far 17 subtypes have been identified within the Blastocystis clades existing in mammals, birds and reptiles. Due to recent studies it has been shown that no group exclusive to humans exists and that human isolates predominantly lie within subtype 1-4 [3][4][5]. Until recently, all human isolates of Blastocystis were commonly referred to as Blastocystis hominis and all other animal isolates were called Blastocystis sp.…”

Section: Introductionmentioning

confidence: 99%

Update on the Molecular Epidemiology and Diagnostic Tools for Blastocystis sp

Roberts¹,

Stark²,

Harkness³

et al. 2014

J Med Microb Diagn

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Blastocystis is the most common enteric protist found in humans. Due to the recent advancements in molecular technologies, up to 17 subtypes have been identified from humans and animals. Molecular epidemiological studies have shown the large range of subtype (ST) distribution within geographical locations with ST1-9 being identified in humans. ST3 has been identified as the predominant subtype in most epidemiological studies with a considerable absence of ST4 noted in Africa and the Middle East compared to Europe and Australia where this subtype is fairly common. This review summarises the molecular tools used for diagnosis and speciation of Blastocystis and comments on advancements in the area over the last 15 years and what future trends may be. This review also describes the geographical distribution of Blastocystis and comments on possible intra-subtype evolutionary relationships.

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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

Cited by 164 publications

References 44 publications

Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples

Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples

Comparative Prevalence of Blastocystis in Patients with the Irritable Bowel Syndrome and Healthy Individuals: A Case Control Study

Update on the Molecular Epidemiology and Diagnostic Tools for Blastocystis sp

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