Phylogenetic analysis and growth profiles of Fusarium incarnatum-equiseti species complex strains isolated from Tunisian cereals

Jedidi, Inès; Jurado, Miguel; Cruz, Alejandra; Trabelsi, M; Saïd, Salem; González-Jaén, M. Teresa

doi:10.1016/j.ijfoodmicro.2021.109297

Cited by 9 publications

(8 citation statements)

References 50 publications

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“…Therefore, molecular methods are essential to concretely identify Fusarium at the species level. An effective method for identifying Fusarium species has been designed using protein-coding ( β-tubulin , cam , tef-1 , and RNA polymerase largest sub-unit) and ribosomal DNA (the internal transcribed spacer and the large sub-unit regions) genes [ 35 , 38 , 42 , 63 , 64 , 65 , 66 ]. However, several previous studies have reported that species-level identification of Fusarium cannot be achieved using only the ribosomal DNA gene [ 67 , 68 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, several previous studies have reported that species-level identification of Fusarium cannot be achieved using only the ribosomal DNA gene [ 67 , 68 ]. Therefore, the accurate identification of Fusarium species is currently carried out using a combination of morphological characteristic and multi-gene molecular phylogenetic analyses [ 35 , 36 , 37 , 38 , 40 , 63 , 64 , 66 ]. In this study, two isolates of F. compactum (SDBR-CMU461 and SDBR-CMU462) and two isolates of F. paranaense (SDBR-CMU463 and SDBR-CMU464) were isolated from fruit rot lesions on watermelons from northern Thailand.…”

Section: Discussionmentioning

confidence: 99%

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Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Nuangmek

Kumla

Khuna

et al. 2023

Plants

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Fruit rot caused by phytopathogenic fungi is one of the major diseases affecting watermelons (Citrullus lanatus) around the world, which can result in unmarketable fruits and significant economic losses. Fruit rot was observed on watermelons throughout the postharvest storage periods in Phayao Province, northern Thailand in 2022. For the present study, a total of ten fungal isolates were isolated from the rot lesions of watermelons. All obtained fungal isolates were then characterized in terms of their pathogenicity. The results indicated that only four fungal isolates caused rot disease with similar symptoms during the postharvest storage period. Based on their morphological characteristics, these four fungal isolates were identified as belonging to the genus Fusarium. Using multi-gene phylogenetic analyses with a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and RNA polymerase second largest subunit (rpb2) genes, the fungal isolates were subsequently identified as Fusarium compactum and F. paranaense. Taken together, the results of this study indicate that F. compactum and F. paranaense cause fruit rot disease in watermelons. To the best of our knowledge, this is the first study to report F. compactum and F. paranaense as novel pathogens of watermelon fruit rot both in Thailand and elsewhere in the world.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Nuangmek

Kumla

Khuna

et al. 2023

Plants

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“…Therefore, it is essential to identify Fusarium species by applying a molecular approach. Ribosomal DNA [the internal transcribed spacer (ITS) and the large subunit (LSU) regions] and protein-coding [cam, tef-1, β-tubulin (tub2), RNA polymerase largest subunit (rpb1 and rpb2)] genes have provided researchers with a powerful tool in the identification of the Fusarium species [30,31,36,49,[63][64][65]. Nevertheless, using only the ribosomal DNA gene did not resolve the identification of Fusarium at the species level [66,67].…”

Section: Discussionmentioning

confidence: 99%

Morphology, Molecular Identification, and Pathogenicity of Two Novel Fusarium Species Associated with Postharvest Fruit Rot of Cucurbits in Northern Thailand

Khuna

Kumla

Thitla

et al. 2022

JoF

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Fruit rot of cucurbits caused by several pathogenic fungi has become an important postharvest disease worldwide. In 2022, fruit rot on watermelon (Citrullus lanatus) and muskmelon (Cucumis melo) was observed during the postharvest storage phase in the Chiang Mai and Phitsanulok Provinces of northern Thailand. These diseases can lead to significant economic losses. This present study was conducted to isolate the causal agent of fungi in lesions of fruit rot. A total of four fungal isolates were obtained, of which two isolates (SDBR-CMU422 and SDBR-CMU423) were obtained from rot lesions of watermelons, while the remaining isolates (SDBR-CMU424 and SDBR-CMU425) were obtained from rot lesions of muskmelons. All fungal isolates were identified using both morphological characteristics and molecular analyses. Morphologically, all isolated fungal isolates were classified into the genus Fusarium. Multi-gene phylogenetic analyses of a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and RNA polymerase second largest subunit (rpb2) genes reveled that four fungal isolates belonged to the Fusarium incarnatum–equiseti species complex and were distinct from all other known species. Thus, we have described them as two new species, namely F. citrullicola (SDBR-CMU422 and SDBR-CMU423) and F. melonis (SDBR-CMU424 and SDBR-CMU425). A full description, illustrations, and a phylogenetic tree indicating the position of both new species have been provided. Moreover, pathogenicity tests were subsequently performed and the results showed that F. citrullicola and F. melonis caused symptoms of fruit rot on inoculated watermelon and muskmelon fruits, respectively. Notably, this outcome was indicative of the symptoms that appeared during the postharvest storage phase. To our knowledge, two new pathogenic fungi, F. citrullicola and F. melonis, are new causal agents of watermelon and muskmelon fruit rot, respectively. Importantly, these findings provide valuable information for the development of effective strategies for the monitoring and prevention of these diseases.

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“…F. sulawense was isolated for the first time on bananas in Indonesia in 2014, and it has been indicated that this species is endemic to this crop [ 30 ]. This species belongs to the F. incarnatum-equiseti complex, which is a phylogenetically rich complex that contains more than 30 pathogenic species [ 39 ]. F. sulawense has been reported to infect numerous crops in tropical and equatorial areas.…”

Section: Discussionmentioning

confidence: 99%

Identification of New Fusarium sulawense Strains Causing Soybean Pod Blight in China and Their Control Using Carbendazim, Dipicolinic Acid and Kojic Acid

Sun

Zhang

Xie

et al. 2022

IJERPH

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Soybean plants are highly susceptible to Fusarium species, which significantly reduce soybean production and quality. Several Fusarium species have been reported to synthesize mycotoxins, such as trichothecene, which have been related to major human diseases. In November 2021, soybean pods in Nantong municipality, China, showed black necrotic lesions during the harvest stage. The disease incidence reached 69%. The pathogen was identified as Fusarium sulawense via morphological analysis and sequencing of ITS, EF1-α and RPB2 genes. A PCR assay with primers targeting the trichothecene biosynthesis genes suggested that the three isolates could synthesize trichothecenes. The effectiveness of fungicide carbendazim and natural metabolites dipicolinic acid and kojic acid was screened for the management of F. sulawense on postharvest soybean pods. The highest efficacy was obtained when combining 3.8 mg/mL carbendazim and 0.84 mg/mL dipicolinic acid (curative efficacy: 49.1% lesion length inhibition; preventive efficacy: 82.7% lesion length inhibition), or 1.9 mg/mL carbendazim and 0.71 mg/mL kojic acid (preventive efficacy: 84.9% lesion length inhibition). Collectively, this report will lead to a better understanding of the safety hazards found in soybean products in China and reveals the application of dipicolinic and kojic acids to reduce the use of carbendazim.

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Phylogenetic analysis and growth profiles of Fusarium incarnatum-equiseti species complex strains isolated from Tunisian cereals

Cited by 9 publications

References 50 publications

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Morphology, Molecular Identification, and Pathogenicity of Two Novel Fusarium Species Associated with Postharvest Fruit Rot of Cucurbits in Northern Thailand

Identification of New Fusarium sulawense Strains Causing Soybean Pod Blight in China and Their Control Using Carbendazim, Dipicolinic Acid and Kojic Acid

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