Phylodynamics of HIV-1 Circulating Recombinant Forms 12_BF and 38_BF in Argentina and Uruguay

Bello, Gonzalo; Aulicino, Paula; Ruchansky, Dora; Guimarães, Monick Lindenmayer; López-Galı́ndez, Cecilio; Casado, Concepción; Chiparelli, Héctor; Rocco, Carlos; Mangano, Andrea; Sen, Luisa; Morgado, Mariza G.

doi:10.1186/1742-4690-7-22

Cited by 48 publications

(41 citation statements)

References 48 publications

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“…Our findings are in line with the fact that subtype B has a lower vitality when exposed to Nelfinavir than AE variants [34]. Moreover, BF CRF forms with F protease show a high rate of growth rate in Argentina [35] and Uruguay [36] which would be explained to some extent by a higher F proteases vitality.…”

Section: Overall Differences Between Subtype B and Subtype F Proteasessupporting

confidence: 87%

Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Iamarino¹

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Section: Overall Differences Between Subtype B and Subtype F Proteasessupporting

confidence: 87%

Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Iamarino¹

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“…The full-length sequencing allowed the characterization of the recombinant CRF12_B/F1 together with other diverse URF_B/F1 7,8 , showing that almost all F1 strains, according to the env gene, were in fact recombinant forms with breaking points with subtype B in the pol gene. Other studies using only strains from Buenos Aires detected recombinants URF_B/F1, CRF16_A2D, B/D, B/C, B/K, B/A, CRF06_cpx, and a triple recombinant B/C/F 9,10,11,12,13 . The epidemic in Buenos Aires has been exhaustively studied, but little is known about the HIV-1 epidemic in the rest of Argentina.…”

Section: Introductionmentioning

confidence: 95%

Caracterización de los subtipos de VIH-1 presentes en Córdoba (Argentina) desde casi el inicio de la infección, utilizando los genes POL y ENV.

Ittig

Lucca

Caeiro

et al. 2017

Rev Fac Cien Med Univ Nac Cordoba

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Abstract:Objective: In the present study we characterize, using a retrospective approach, the HIV-1 infection in Córdoba, Argentina, by determining the viral subtypes circulating in the period 1986-2001. Methods: The pol and env genes were analyzed using two different approaches: 1) comparing the sequences from Córdoba with those of pure subtypes and circulating recombinant forms of the HIV-1 M group; 2) subdividing the sequences into pure-subtype data sets (avoiding the effects of recombination) to determine if the subtypes from Córdoba have a single or multiple geographic origins. Results: The first approach revealed that eighteen strains were subtype B, seven B/F1, one C and one F1. Only two of the B/F1 corresponded to the CRF12_B/F1, previously described for Argentina; the remaining B/F1 sequences could be considered different forms of URF_B/F1. The Phylogenetic results of the second approach suggest that in Córdoba subtype B would have multiple geographic origins, while subtypes F1 and C would have been introduced directly from Brazil or indirectly from Buenos Aires. Conclusions: The great subtype variability indicates that the HIV-1 infection in Córdoba was complex since its very beginning. Our results also reveal the close contact of people of this city, through tourism and commercial activities, with those from Buenos Aires and Brazil. Keywords:HIV-1 infection; Córdoba, Argentina; HIV-1 subtypes; phylogenetic analyses; inter subtype recombinant strains Resumen:Objetivo: En el presente estudio, utilizando un enfoque retrospectivo, caracterizamos la infección de VIH-1 en Córdoba (Argentina) a través de la determinación de los subtipos virales circulantes en el período 1986-2001. Métodos: Se analizaron los genes pol y env utilizando dos enfoques diferentes: 1) comparando las secuencias de Córdoba con aquellas de subtipos puros y formas recombinantes circulantes del grupo M del VIH-1; 2) subdividiendo las secuencias en matrices de subtipos puros (evitando los efectos de la recombinación) a fin de determinar si los subtipos de Córdoba tienen uno o varios orígenes geográficos. Resultados: El primer enfoque reveló que dieciocho cepas correspondían al subtipo B, siete a B/F1, una a C y una a F1. Solo dos de los B/F1 correspondían a la forma CRF12_B/F1, previamente descripta para Argentina; las secuencias B/F1 restantes serían diferentes formas de URF_B/F1. Los análisis filogenéticos del segundo enfoque sugieren que el subtipo B de Córdoba tendría múltiples orígenes geográficos, mientras que los subtipos F1 y C habrían sido introducidos directamente desde Brasil o indirectamente desde Buenos Aires. Conclusiones: La gran variabilidad de subtipos indica que la infección de VIH-1 en Córdoba fue compleja desde sus inicios. Nuestros resultados también revelan el estrecho contacto entre la gente de esta ciudad, a través del turismo y actividades comerciales, con las de Buenos Aires y Brasil.

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“…First, a high rate of population growth was observed for both CRF12_BF and CRF38_BF in Argentina and Uruguay. 24,25 Second, it was demonstrated that the BF recombinant LTR/Tat complex has higher transcriptional activity compared to the subtype B complex 26 and that BF Vpu-harboring variants have increased fitness compared to subtype B in vitro. 27 Nevertheless, it remains to be investigated to what extent these results could be generalized to other BF recombinants spreading in South America.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Primary Isolates of HIV Type 1 CRF28_BF, CRF29_BF, and Unique BF Recombinants Circulating in São Paulo, Brazil

Melo

Jamal²,

Zanotto

2012

AIDS Research and Human Retroviruses

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We report for the first time the genetic and biological characterization of 10 HIV-1 primary isolates representing CRF28_BF and CRF29_BF together with additional unique BF recombinant forms (URFs) obtained by PBMC cocultivation. Recombination is an important factor promoting the increase in the genetic diversity of HIV-1. Notably, more than 20% of HIV-1 sequences worldwide were recombinants. Several recombinant viruses were reported in Brazil, and six circulating recombinant forms (CRFs) have been identified (CRF28_BF, CRF29_BF, CRF31_BC, CRF39_BF, CRF40_BF, and CRF46_BF). CRF28_BF and CRF29_BF were found to infect almost 30% of the patients in Sã o Paulo State. The near full-length genomes of these 10 primary isolates were amplified by nested PCR in three overlapping segments, purified, and sequenced. Three samples were related to CRF28_BF, three to CRF29_BF, and four were unique recombinant forms (URFs), as determined by their breakpoint profile determined with the jpHMM program. Additionally, the coreceptor usage of these isolates was investigated in vitro using GHOST assays, which revealed three dual-tropic (X4/R5) viruses, four lymphotropic (X4) viruses, and three macrophage-tropic (R5) viruses with different V3-loop motifs, which challenges the notion that GWGR-carrying viruses are macrophage-tropic only. In sum, we report a much-anticipated well-characterized panel of viruses representing CRF28_BF, CRF29_BF, and URFs from Sã o Paulo State, Brazil.

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Phylodynamics of HIV-1 Circulating Recombinant Forms 12_BF and 38_BF in Argentina and Uruguay

Cited by 48 publications

References 48 publications

Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Caracterización de los subtipos de VIH-1 presentes en Córdoba (Argentina) desde casi el inicio de la infección, utilizando los genes POL y ENV.

Characterization of Primary Isolates of HIV Type 1 CRF28_BF, CRF29_BF, and Unique BF Recombinants Circulating in São Paulo, Brazil

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