Phyllotreta striolata (Coleoptera: Chrysomelidae): Arginine kinase cloning and RNAi-based pest control

Zhao, Yi-Ying; Guang, Yang; Wang‐Pruski, Gefu; You, Minsheng

doi:10.14411/eje.2008.108

Cited by 71 publications

(39 citation statements)

References 36 publications

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“…Genes encoding a vacuolar V-ATPase and AK were chosen based on previous successful reports of RNAi used for insect control (Baum et al, 2007; Zhao et al, 2008; Kola et al, 2015). Since no sequences were available for T .…”

Section: Methodsmentioning

confidence: 99%

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resulting in larval phenotypes that range from loss of appetite to death. In this report, we show that the tomato leafminer (Tuta absoluta), a major threat to commercial tomato production, can be targeted by RNAi. We selected two target genes (Vacuolar ATPase-A and Arginine kinase) based on the RNAi response reported for these genes in other pest species. In view of the lack of an artificial diet for T. absoluta, we used two approaches to deliver dsRNA into tomato leaflets. The first approach was based on the uptake of dsRNA by leaflets and the second was based on “in planta-induced transient gene silencing” (PITGS), a well-established method for silencing plant genes, used here for the first time to deliver in planta-transcribed dsRNA to target insect genes. Tuta absoluta larvae that fed on leaves containing dsRNA of the target genes showed an ∼60% reduction in target gene transcript accumulation, an increase in larval mortality and less leaf damage. We then generated transgenic ‘Micro-Tom’ tomato plants that expressed hairpin sequences for both genes and observed a reduction in foliar damage by T. absoluta in these plants. Our results demonstrate the feasibility of RNAi as an alternative method for controlling this critical tomato pest.

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Section: Methodsmentioning

confidence: 99%

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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“…By comparison, siRNA is easy to synthesise and is becoming less costly. The smaller siRNA molecule could also be designed to be degradation resistant and to have enhanced stability . The application of siRNA is popular nowadays, and some therapy agents for hereditary diseases have even been developed .…”

Section: Introductionmentioning

confidence: 99%

Comparison of the RNA interference effects triggered by dsRNA and siRNA in Triboliumcastaneum

Wang

et al. 2013

Pest Management Science

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“…striolata (Coleoptera: Chrysomelidae), is one of the most serious pests of Cruciferae crops worldwide (Andersen et al ., 2006). It is the most significant vegetable crop pest in Southern China and is resistant to Bt (Zhao et al ., 2008).…”

Section: Introductionmentioning

confidence: 99%

PsOr1, a potential target for RNA interference‐based pest management

Zhao

Liu

Guang

et al. 2010

Insect Molecular Biology

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Insect pests cause billions of dollars in agricultural losses, and attempts to kill them have resulted in growing threats from insecticide resistance, dietary pesticide pollution and environmental destruction. New approaches to control refractory insect pests are therefore needed. The host-plant preferences of insect pests rely on olfaction and are mediated via a seven transmembrane-domain odorant receptor (Or) family. The present study reports the cloning and characterization of PsOr1, the first candidate member of the Or gene family from Phyllotreta striolata, a devastating beetle pest that causes damage worldwide. PsOr1 is remarkably well conserved with respect to other insect orthologues, including DmOr83b from Drosophila melanogaster. These insect orthologues form an essential non-conventional Or sub-family and may play an important and generalized role in insect olfaction. We designed double-stranded (ds) RNA directly against the PsOr1 gene and exploited RNA interference (RNAi) to control P. striolata. The chemotactic behavioural measurements showed that adult beetles were unable to sense the attractant or repellent odour stimulus after microinjection of dsRNA against PsOr1. Reverse Transcription (RT)-PCR analysis showed specific down-regulation of mRNA transcript levels for this gene. Furthermore, host-plant preference experiments confirmed that silencing PsOr1 by RNAi treatment impaired the host-plant preferences of P. striolata for cruciferous vegetables. These results demonstrate that this insect control approach of using RNAi to target PsOr1 and its orthologues might be effective in blocking host-plant-seeking behaviours in diverse insect pests. The results also support the theory that this unique receptor type plays an essential general role in insect olfaction.

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Phyllotreta striolata (Coleoptera: Chrysomelidae): Arginine kinase cloning and RNAi-based pest control

Cited by 71 publications

References 36 publications

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Comparison of the RNA interference effects triggered by dsRNA and siRNA in Triboliumcastaneum

PsOr1, a potential target for RNA interference‐based pest management

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