Phthalocyanine–DNA origami complexes with enhanced stability and optical properties

Abstract

<p>Cationic phthalocyanines bind DNA origami nanostructures, which protects them against enzymatic degradation and enhances the optical properties of the phthalocyanines.</p>

Prospective Cancer Therapies Using Stimuli‐Responsive DNA Nanostructures

“…It was recently shown that DNA-based carrier complexed with photosensitizers DNs may also provide protection from endonuclease degradation, which could then provide a key to prolonged circulation times in the body. [123] AuNPs, in turn, have been at the forefront of creating multifunctional nanoplatforms that allow simultaneous cancer diagnosis and therapy. However, lack of tumor penetration, specificity, modification optimization, and size-irregularities of the AuNPs calls for the use of a nanocarrier that can impart uniform shape and size with precise spatial addressability.…”

Biomolecule‐Directed Carbon Nanotube Self‐Assembly

“…[66] The unique programmability and addressability of the DNA origami [67] have been exploited for a wide range of applications, including controlled drug delivery, targeting, and cargo release. [68][69][70][71][72] Higher-ordered structures [73] are achieved by hybridization with small molecules, [74] inorganic nanoparticles, [75] or DNA-functionalized SWCNTs. The latter were reported to self-assemble and align as cross-junctions on DNA origami tiles of 75 × 95 nm 2 ( Figure 3d).…”

DNA‐Origami‐Templated Growth of Multilamellar Lipid Assemblies

“…In general, the DNAorigami is more resilient to nuclease digestion than simple double-stranded DNAo fs imilar sizes, [50] however, bare DNAo rigami still has rather limited resistance against enzymatic degradation in biologically relevant media. [18,27] As previously reported, various strategies have proven to increase the structural stability against nucleases, [28][29][30][32][33][34][35][36][37][38] and therefore,w ea lso examined here whether the DNAorigami embedded in the lipid matrix could be protected against deoxyribonuclease I( DNase I) digestion. DNase Ii st he most important and active nuclease in blood, serum and mammalian cells,a nd therefore the resiliency to DNase Ii sw idely used as am easure to predict and also simulate the DNAorigami stability in vivo.…”

“…[25] However,f or many potential applications,inparticular in biomedicine,the structural stability of the DNAo rigami is still persuading asevere challenge,astheDNA origami structures are prone to degradation by nucleases,such as DNase I. [26,27] Therefore, to increase the stability of the DNAorigami structures under biologically relevant conditions,s everal coating strategies have been proposed, including viral envelope-mimicking lipid bilayers, [28] glutaraldehyde cross-linked PEGylated oligolysines, [29] covalent photo-crosslinking of thymidines by the formation of cyclobutane pyrimidine dimers, [30] and electrostatically driven coatings with cationic polymers, [31][32][33][34] peptoids, [35] serum albumin-dendron conjugates [36] and -derivatives, [37] phthalocyanines, [38] virus capsid proteins, [39] and chitosan. [34] In this work, we have electrostatically assembled three different DNAo rigami nanostructures with ac ationic lipid 1,2-dioleoly-3-trimethylammonium-propane (DOTAP) (Fig-ure 1).…”