DNA‐Origami‐Templated Growth of Multilamellar Lipid Assemblies

Abstract

Lipids are important building blocks in cellular compartments, and therefore their self‐assembly into well‐defined hierarchical structures has gained increasing interest. Cationic lipids and unstructured DNA can co‐assemble into highly ordered structures (lipoplexes), but potential applications of lipoplexes are still limited. Using scaffolded DNA origami nanostructures could aid in resolving these drawbacks. Here, we have complexed DNA origami together with a cationic lipid 1,2‐dioleoly‐3‐trimethylammonium‐propane (DOTAP) and studied their self‐assembly driven by electrostatic and hydrophobic interactions. The results suggest that the DNA origami function as templates for the growth of multilamellar lipid structures and that the DNA origami are embedded in the formed lipid matrix. Furthermore, the lipid encapsulation was found to significantly shield the DNA origami against nuclease digestion. The presented complexation strategy is suitable for a wide range of DNA‐based templates and could therefore find uses in construction of cell‐membrane‐associated components.

Liposomal Delivery of MicroRNA-7 Targeting EGFR to Inhibit the Growth, Invasion, and Migration of Ovarian Cancer

“…In a wide pH range, DOTAP is positively charged. 17 Therefore, the above liposomes have a certain positive charge to attract negatively charged mRNA. Importantly, an excessive positive charge will produce greater cytotoxicity, and cationic DOTAP liposomes alone have poor stability, 4 so we added a certain proportion of DOPE and CHOL when preparing the liposomes.…”

A Janus-Type Phthalocyanine for the Assembly of Photoactive DNA Origami Coatings

“…The reversibility of this interaction was characterized by EMSA of 1 –24HB, titrated with negatively charged heparin ( Figure 4 c). This biopolymer presents a high density of negative charges (2.7 sulfate groups per disaccharide), 41 , 42 establishing a competitive binding with 1 . Thus, the employed 18 kDa heparin molecules contain, on average, 90 negative charges, which can bind up to 23 tetracationic ZnPc units.…”

Hybrid Nanoassemblies from Viruses and DNA Nanostructures

“…DNA nanostructures can also bind to charged lipid molecules via electrostatic interactions. Julin et al [47] complexed DNA origami together with cationic lipids into lipoplexes and characterized their self-assembly. Three DNA origami designs, 6-helix bundle In the same study, Zhou et al also created more intricate and programmed structures by changing the docking sites of the RNA strands on various DNA origami structures.…”

“…DNA nanostructures can also bind to charged lipid molecules via electrostatic interactions. Julin et al [47] complexed DNA origami together with cationic lipids into lipoplexes and characterized their self-assembly. Three DNA origami designs, 6-helix bundle (6HB), 60HB [48], and a nanoplate were assembled with cationic lipid molecules 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP).…”